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MABT1332-25UG Anti-Tspan-8 Antibody, clone 40/15-1B11-63-76-8

Anti-Tspan-8, clone 40/15-1B11-63-76-8 (Anti-Tspan8-A), Cat. No. MABT1332, is a rat monoclonal antibody that detects Tetraspanin-8 and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

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MABT1332-25UG
25 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABT1332-25UG
      DescriptionAnti-Tspan-8 Antibody, clone 40/15-1B11-63-76-8
      Alternate Names
      • Tetraspanin-8
      • Tspan-8
      Background InformationTetraspanin-8 (UniProt: Q8R3G9; also known as Tspan8, Tspan-8) is encoded by the Tspan8 gene (Gene ID: 216350) in murine species. Tspan-8 is a multi-pass membrane protein of the tetraspanin family that is involved in the regulation of cell activation, growth, and motility. Members of this family have emerged as important regulators of expression levels, trafficking or post-translational modification of laminin-binding integrins. Tetraspanins are shown to associate with one another and cluster dynamically with a large variety of transmembrane and signal-transducing partners, forming specialized membrane microdomains called tetraspanin web. Tspan-8 is reported to be overexpressed during the progression of colorectal, liver, pancreatic and gastric cancers and its increased expression is shown to promote liver and lung metastasis. Overexpression of Tspan-8 has also been correlated with poor prognosis in human colorectal cancer. In metastatic pancreatic and colorectal carcinoma cell lines, PKC activation enhances the colocalization of Tspan-8 and Tspan CD151 with integrin alpha6beta 4 and subsequent internalization of this integrin-tetraspanin complex, resulting in decreased cell-matrix adhesion and increased cell migration. Tspan-8 knockdown in HT29 colon cancer cells is reported to significantly reduce cell migratory ability as a result of an upregulation of both integrin-dependent cell-matrix adhesion and calcium-dependent cell-cell adhesion. (Ref.: Kharbili, ME., et al. (2017). Oncotarget. 8(10); 17140-17155).
      References
      Product Information
      FormatPurified
      PresentationPurified rat monoclonal antibod yIgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-Tspan-8, clone 40/15-1B11-63-76-8 (Anti-Tspan8-A), Cat. No. MABT1332, is a rat monoclonal antibody that detects Tetraspanin-8 and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.
      Key Applications
      • Immunohistochemistry (Paraffin)
      • Western Blotting
      Application NotesImmunohistochemistry Analysis: A representative lot detected Tspan-8 in Immunohistochemistry applications (Fu, N.Y., et. al. (2017). Nat Cell Biol. 19(3):164-176).

      Western Blotting Analysis: 4 µg/mL from a representative lot detected Tspan-8 in LIM1215 positive for Tspan8 and HEK293T cell lysates.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to 10 amino acids from the extracellular domain of murine Tetraspanin-8.
      Epitopeextracellular domain
      Clone40/15-1B11-63-76-8
      ConcentrationPlease refer to lot specific datasheet.
      HostRat
      SpecificityClone 40/15-1B11-63-76-8 is a rat monoclonal antibody that detects Tetraspanin-8. It targets an epitope with in the extracellular domain.
      IsotypeIgG2aκ
      Species Reactivity
      • Mouse
      Species Reactivity NoteMouse. Predicted to react with Human, Monkey based on 100% sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Tspan8
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight25.58 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunohistochemistry (Paraffin) in mouse colon tissue sections.

      Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution of this antibody detected Tspan-8 in mouse colon tissue sections.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      MABT1332-25UG 04054839755262

      Documentation

      Anti-Tspan-8 Antibody, clone 40/15-1B11-63-76-8 Certificates of Analysis

      TitleLot Number
      Anti-Tspan-8, clone 40/15-1B11-63-76-8 - 3208234 3208234

      References

      Reference overviewPub Med ID
      Identification of quiescent and spatially restricted mammary stem cells that are hormone responsive.
      Fu, NY; Rios, AC; Pal, B; Law, CW; Jamieson, P; Liu, R; Vaillant, F; Jackling, F; Liu, KH; Smyth, GK; Lindeman, GJ; Ritchie, ME; Visvader, JE
      Nat Cell Biol  19  164-176  2017

      Show Abstract       		28192422 28192422