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MAB131890-25UG Anti-Venus (YFP/GFP) Antibody, clone 10

Anti-Venus (YFP/GFP), clone 10, Cat. No. MAB131890, is a mouse monoclonal antibody that detects proteins labeled with mVenus and GFP and is tested for use in Immunocytochemistry, Immunoprecipitation, and Western Blotting.

MAB131890-25UG
25 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMAB131890-25UG
      DescriptionAnti-Venus (YFP/GFP) Antibody, clone 10
      Alternate Names
      • YFP/GFP
      Background InformationGreen fluorescent protein (UniProt: P42212; also known as GFP) is encoded by the GFP gene in Aequorea victoria (Jelly Fish). GFP is a 27 kDa protein from jellyfish that serves as an energy-transfer acceptor. It transduces the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer and it fluoresces in vivo upon receiving energy from the Ca2+-activated photoprotein aequorin. GFP has an absorption max at 395 nm and the emission peaks at 507 to 510 nm. However, the exact value of emission max depends on the environment of the chromophore. GFP has evolved into an important tool in cell biology research that displays stability under fixation conditions and is suitable for a variety of applications. mVenus is a genetic mutant with F46L substitution in green fluorescent protein (GFP), derived from Aequorea victoria. Its excitation/emission peaks are 515/528 nm respectively and is often used as a donor-acceptor pair by researchers in FRET based co-localization studies. Compared to other fluorescent tags, Venus provides higher expression levels and better yields in preparative purifications. A fusion protein consisting of Venus and a defective mutant of p27 protein (p27K ) has been used to identify and isolate a population of quiescent cells and visualize the G0 to G1 transition. Clone 10 detects both Venus and GFP-tagged proteins. (Ref.: Nagai, T., et al. (2002). Nat. Biotechnol. 20(1); 87-90; Oki, T., et al. (2014). Sci. Rep. 4; Article 4012; Rana, MS., et al. (2018). Biochemistry 57(49); 6741-6751).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-Venus (YFP/GFP), clone 10, Cat. No. MAB131890, is a mouse monoclonal antibody that detects proteins labeled with mVenus and GFP and is tested for use in Immunocytochemistry, Immunoprecipitation, and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      • Immunoprecipitation
      Application NotesWestern Blotting Analysis: A 1:500 dilution from a representative lot detected Venus (YFP/GFP) lebeled proteins in lysate from HEK293T cells transfected with mVenus that was expressed using pCAGGS-Myc eukaryotic expression plasmid (Courtesy of Li Ten and Kensaku Murano, Ph.D., Keio University School of Medicine, Tokyo Japan).

      Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected Venus (YFP/GFP) labeled proteins in NIH3T3 (L20V-9-11) cells (Courtesy of Li Ten and Kensaku Murano, Ph.D., Keio University School of Medicine, Tokyo Japan).

      Immunoprecipitation Analysis: A 1:1,000 dilution from a representative lot immunoprecipitated proteins labeled with Venus (YFP/GFP) in pCAGGS-myc (negative) and pCAGGS-myc-mVenus (positive) HEK293T cell lysates (Courtesy of Li Ten and Kensaku Murano, Ph.D., Keio University School of Medicine, Tokyo Japan).
      Biological Information
      ImmunogenGST‐tagged full-length mVenus, a genetic mutant of GFP derived from Aequorea Victoria (Jelly fish).
      EpitopeUnknown
      Clone10
      Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone 10 is a mouse monoclonal antibody that detects proteins labeled with Venus and GFP..
      IsotypeIgG2aκ
      Species Reactivity
      • All
      Species Reactivity NoteAequorea victoria. Predicted to react with all species.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • GFP
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~35 kDa observed; 26.88 kDa calculated for GFP. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceIsotype testing: Identity Confirmation by Isotyping Test.

      Isotyping Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be mouse IgG2a.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at +2°C to +8°C from date of receipt.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      MAB131890-25UG 04065265237734