Skip to Content
MilliporeSigma
All Photos(1)

Key Documents

MABN2552

Sigma-Aldrich

Anti-alpha B-crystallin Antibody, clone aB1788G7G6

Sign Into View Organizational & Contract Pricing


About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Pricing and availability is not currently available.

antibody form

purified antibody

clone

aB1788G7G6
monoclonal

purified by

using protein G

species reactivity

human, mouse, mouse

concentration

(Please refer to lot specific datasheet.)

technique(s)

ELISA: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
inhibition assay: suitable
western blot: suitable

isotype

IgG1κ

UniProt accession no.

General description

KLH-conjugated linear peptide corresponding to 21 amino acids from the N-terminal half of human Alpha(B)-crystallin.

Specificity

Clone aB1788G7G6 is a mouse monoclonal antibody that detects Alpha(B)-crystallin. It targets an epitope within 21 amino acids within the N-terminal half.

Application

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected alpha B-crystallin in mouse eye and human retina tissue lysate.

Immunohistochemistry Analysis: A representative lot detected alpha B-crystallin in Immunohistochemistry applications (Nahomi, R.B., et. al. (2019). J. Immunol. Methods. 467; 37-47).

ELISA Analysis: A representative lot detected alpha B-crystallin in ELISA applications (Nahomi, R.B., et. al. (2019). J. Immunol. Methods. 467; 37-47).

Inhibition Analysis: A representative lot inhibited the chaperone and anti-apoptotic activities of alpha B-crystallin. (Nahomi, R.B., et. al. (2019). J. Immunol. Methods. 467; 37-47).

Immunofluorescence Analysis: A representative lot detected alpha B-crystallin in Immunofluorescence applications (Nahomi, R.B., et. al. (2019). J. Immunol. Methods. 467; 37-47).

Western Blotting Analysis: A representative lot detected alpha B-crystallin in Western Blotting applications (Nahomi, R.B., et. al. (2019). J. Immunol. Methods. 467; 37-47).

Quality

Evaluated by Western Blotting in mouse retina tissue lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected alpha B-crystallin in mouse retina tissue lysate.

Physical properties

~20 kDa observed; 20.16 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Physical form

Purified mouse monoclonal antibody IgG1 in PBS without preservatives.

Storage and Stability

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Other Notes

Human, Mouse. Predicted to react with Bovine, Monkey, Rat based on 100% sequence homology.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service