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OP23 Anti-c-H-Ras (Ab-1) Mouse mAb (F235-1.7.1)

This Anti-c-H-Ras (Ab-1) Mouse mAb (F235-1.7.1) is validated for use in Frozen Sections, Immunoblotting, IF, IP, Paraffin Sections for the detection of c-H-Ras (Ab-1).

Anti-c-H-Ras (Ab-1) Mouse mAb (F235-1.7.1) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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OP23
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Overview

Replacement Information

Key Specifications Table

Species ReactivityHostAntibody Type
H, M, RMMonoclonal Antibody

Pricing & Availability

Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
OP23-100UG
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      		 Plastic ampoule 100 μg
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      OP23-200UG
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      Limited Availability Limited Availability
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      Limited Quantities Available
      Available
        Remaining : Will advise
          Remaining : Will advise
          Will advise
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          		 Plastic ampoule 200 μg
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          Description
          OverviewRecognizes the ~21 kDa c-H-Ras protein in normal skin tissue and Rat1 cells. Does not react with c-K-Ras or c-N-Ras p21. A doublet may be seen due to farnesylation.
          Catalogue NumberOP23
          Brand Family Calbiochem®
          References
          ReferencesRodenhuis, S. et al. 1992. Cancer Res. (Suppl) 52, 2665s.
          Sidransky, D., et al. 1992. Science 256, 102.
          Bos, J.L. 1989. Cancer Res. 49, 4682.
          Furth, M. E., et al. 1987. Oncogene 1, 47.
          Kraus, M.H., et al. 1984. Proc. Natl. Acad. Sci. USA 81, 5384.
          Shimizu, K., et al. 1983. Proc. Natl. Acad. Sci. USA 80, 2112.
          Taparowsky, E., et al. 1983. Cell 34, 581.
          Ellis, R.W., et al. 1981. Nature 292, 506.
          Shih, T.Y., et al. 1980. Nature 287, 686.
          Product Information
          FormLiquid
          FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.4.
          Positive controlNormal skin tissue or ras 1 cells
          Preservative≤0.1% sodium azide
          Quality LevelMQ100
          Applications
          Key Applications Frozen Sections
          Immunoblotting (Western Blotting)
          Immunofluorescence
          Immunoprecipitation
          Paraffin Sections
          Application NotesFrozen Sections (5 µg/ml)
          Immunoblotting (10 µg/ml)
          Immunofluorescence (2.5 µg/ml)
          Immunoprecipitation (5 µg/ml)
          Paraffin Sections (5 µg/ml, saponin and pepsin pre-treatment required)
          Application CommentsFor immunoprecipitation, use 5 µg of Cat. No. OP23 per sample with 45 µl protein G plus agarose. The level of expression of p21Ras is variable in different tissues. For this reason, we recommend a concentration step prior to immunoblotting analysis to obtain optimal results. A doublet may be seen due to farnysylation. Recognizes c-H-Ras and, weakly, v-H-Ras; does not recognize either c-K-Ras or c-N-Ras p21s under the conditions tested. Antibody should be titrated for optimal results in individual systems.
          Biological Information
          Immunogenrecombinant p21 Ras
          Epitopewithin amino acids 54-188
          CloneF235-1.7.1
          HostMouse
          IsotypeIgG₁
          Species Reactivity
          • Human
          • Mouse
          • Rat
          Antibody TypeMonoclonal Antibody
          Concentration Label Please refer to vial label for lot-specific concentration
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Standard Handling
          Storage +2°C to +8°C
          Do not freeze Yes
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications
          Global Trade Item Number
          Catalog Number GTIN
          OP23-100UG 04055977208962
          OP23-200UG 04055977208986

          Documentation

          Anti-c-H-Ras (Ab-1) Mouse mAb (F235-1.7.1) SDS

          Title

          Safety Data Sheet (SDS) 

          Anti-c-H-Ras (Ab-1) Mouse mAb (F235-1.7.1) Certificates of Analysis

          TitleLot Number
          OP23

          References

          Reference overview
          Rodenhuis, S. et al. 1992. Cancer Res. (Suppl) 52, 2665s.
          Sidransky, D., et al. 1992. Science 256, 102.
          Bos, J.L. 1989. Cancer Res. 49, 4682.
          Furth, M. E., et al. 1987. Oncogene 1, 47.
          Kraus, M.H., et al. 1984. Proc. Natl. Acad. Sci. USA 81, 5384.
          Shimizu, K., et al. 1983. Proc. Natl. Acad. Sci. USA 80, 2112.
          Taparowsky, E., et al. 1983. Cell 34, 581.
          Ellis, R.W., et al. 1981. Nature 292, 506.
          Shih, T.Y., et al. 1980. Nature 287, 686.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision02-October-2007 RFH
          ApplicationFrozen Sections (5 µg/ml)
          Immunoblotting (10 µg/ml)
          Immunofluorescence (2.5 µg/ml)
          Immunoprecipitation (5 µg/ml)
          Paraffin Sections (5 µg/ml, saponin and pepsin pre-treatment required)
          DescriptionPurified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with P3X63 Ag8.653 mouse myeloma cells. Recognizes the ~21 kDa c-H-Ras protein.
          BackgroundThe human ras gene family consists of three identified members, H, K and N-ras, encoding proteins of 188-189 amino acids and 21,000 (p21) molecular weight. Human H- and K-Ras are the homologues of v-H- and v-K-Ras sequences originally isolated from Harvey and Kirsten strains of rat sarcoma viruses. Normal human cellular ras genes can be activated to oncogenes by mutations occurring in codons 12, 13 and 61; such mutated, activated and transforming ras genes have been identified and isolated from human tumors and cultured tumor cells. Although the expression patterns of Ras proteins in normal human tissues are known, similar information for activated ras oncogene encoded p21's and their relevance to human disease diagnosis and prognosis is still emerging.
          HostMouse
          Immunogenrecombinant p21 Ras
          Epitopewithin amino acids 54-188
          CloneF235-1.7.1
          IsotypeIgG₁
          Specieshuman, mouse, rat
          Positive controlNormal skin tissue or ras 1 cells
          FormLiquid
          FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.4.
          Concentration Label Please refer to vial label for lot-specific concentration
          Preservative≤0.1% sodium azide
          CommentsFor immunoprecipitation, use 5 µg of Cat. No. OP23 per sample with 45 µl protein G plus agarose. The level of expression of p21Ras is variable in different tissues. For this reason, we recommend a concentration step prior to immunoblotting analysis to obtain optimal results. A doublet may be seen due to farnysylation. Recognizes c-H-Ras and, weakly, v-H-Ras; does not recognize either c-K-Ras or c-N-Ras p21s under the conditions tested. Antibody should be titrated for optimal results in individual systems.
          Storage +2°C to +8°C
          Do Not Freeze Yes
          Toxicity Standard Handling
          ReferencesRodenhuis, S. et al. 1992. Cancer Res. (Suppl) 52, 2665s.
          Sidransky, D., et al. 1992. Science 256, 102.
          Bos, J.L. 1989. Cancer Res. 49, 4682.
          Furth, M. E., et al. 1987. Oncogene 1, 47.
          Kraus, M.H., et al. 1984. Proc. Natl. Acad. Sci. USA 81, 5384.
          Shimizu, K., et al. 1983. Proc. Natl. Acad. Sci. USA 80, 2112.
          Taparowsky, E., et al. 1983. Cell 34, 581.
          Ellis, R.W., et al. 1981. Nature 292, 506.
          Shih, T.Y., et al. 1980. Nature 287, 686.