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MABS14 Anti-mTOR Antibody, clone F11

This Anti-mTOR Antibody, clone F11 is validated for use in WB, IP for the detection of mTOR.

Anti-mTOR Antibody, clone F11 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: PI3K, Akt, & mTOR Signaling Gene Symbol: FRAP, FRAP1, FRAP2, RAFT1, RAPT1, mTOR UniProt Number: P42346
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MABS14
100 µg  
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      Overview

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      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, RWB, IPMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABS14
      DescriptionAnti-mTOR Antibody, clone F11
      Alternate Names
      • FK506 binding protein 12-rapamycin associated protein 1
      • FK506 binding protein 12-rapamycin associated protein 2
      • FK506-binding protein 12-rapamycin complex-associated protein 1
      • FKBP-rapamycin associated protein
      • FKBP12-rapamycin complex-associated protein 1
      • Mammalian target of rapamycin
      • Rapamycin target protein
      • mechanistic target of rapamycin (serine/threonine kinase)
      • rapamycin and FKBP12 target 1
      • rapamycin associated protein FRAP2
      Background InformationmTOR (Mammalian Target of Rapamycin, aka FRAP, RAPT or RAFT) is a large 289 kDa Ser/Thr protein kinase that regulates cell cycle progression, cell growth, protein synthesis, ribosome biogenesis, and autophagy. mTOR is an evolutionarily conserved member of the Phosphoinositol Kinase-related Kinase (PIKK) family whose activity is regulated by phosphorylation on Ser2448 by Akt in response to insulin or muscle activity. mTORC1 (mTOR Complex 1) consists of at least mTOR, Raptor, and G (mLST8). mTORC1 is known to play a central role in insulin signaling, which is crucial in maintaining metabolic homeostasis. mTORC2 affects cell proliferation and survival primarily by phosphorylating the hydrophobic motif of Akt on Ser473, a well-known effecter of the PI3 Kinase pathway. In addition to phosphorylating Akt, the mTORC2 complex is also known to effect cytoskeletal organization and migration by exerting its effects through Rac, Rho, and PKC. Defects in both mTOR complexes are associated with a variety of diseases, including cancer and diabetes.
      References
      Product Information
      FormatPurified
      Control
      • HeLa cell lysate
      PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-mTOR Antibody, clone F11 is validated for use in WB, IP for the detection of mTOR.
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      Application NotesImmunoprecipitation Analysis: A previous lot was used by an independent laboratory in IP. (Nemazanyy, I, et al. (2008). Hybridoma, 27:395-398.)
      Biological Information
      ImmunogenGST-tagged recombinant protein corresponding to rat mTOR.
      CloneF11
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      IsotypeIgG1κ
      Species Reactivity
      • Human
      • Rat
      Species Reactivity NoteDemonstrated to react with human. Predicted to react with rat based on 100% sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryBinds the complex formed by the immunosuppressive drug rapamycin and its receptor FKBP12; may play a role in the cell cycle G1 to S transition [RGD].
      Gene Symbol
      • FRAP
      • FRAP1
      • FRAP2
      • RAFT1
      • RAPT1
      • mTOR
      Purification MethodProtein G
      UniProt Number
      UniProt SummaryFUNCTION: Kinase subunit of both mTORC1 and mTORC2, which regulate cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino-acids. Amino-acid-signaling to mTORC1 is mediated by Rag GTPases, which cause amino-acid-induced relocalization of mTOR within the endomembrane system. Growth factor-stimulated mTORC1 activation involves AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-421', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. mTORC2 is also activated by growth factors, but seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-421'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657' (By similarity).

      CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein.

      ENZYME REGULATION: Kinase activity is positively regulated by RHEB and negatively regulated by DEPDC6 (By similarity).

      SUBUNIT STRUCUTRE: Part of the mammalian target of rapamycin complex 1 (mTORC1) which contains MTOR/FRAP1, MLST8, RPTOR and AKT1S1 By similarity. mTORC1 binds to and is inhibited by FKBP12-rapamycin. Part of the mammalian target of rapamycin complex 2 (mTORC2) which contains FRAP1, MLST8, PRR5, RICTOR and MAPKAP1 (By similarity). Contrary to mTORC1, mTORC2 does not bind to and is not sensitive to FKBP12-rapamycin. Binds directly to PRR5 and RICTOR within the mTORC2 complex (By similarity). Interacts with UBQLN1 (By similarity). Interacts with DEPDC6 (By similarity). Interacts with PPAPDC3 By similarity. Interacts with TTI1 and TELO2 (By similarity).

      SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side (By similarity). Golgi apparatus membrane; Peripheral membrane protein; Cytoplasmic side (By similarity). Mitochondrion outer membrane; Peripheral membrane protein; Cytoplasmic side (By similarity).

      PTM: Autophosphorylated; when part of mTORC1 or mTORC2 (By similarity).

      SEQUENCE SIMILARITIES: Belongs to the PI3/PI4-kinase family.

      Contains 1 FAT domain.

      Contains 1 FATC domain.

      Contains 7 HEAT repeats.

      Contains 1 PI3K/PI4K domain.
      Molecular Weight~ 289 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in HeLa cell lysate.

      Western Blot Analysis: 0.5 µg/mL of this antibody detected mTOR on 10 µg of HeLa cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      MABS14 04053252290954

      Documentation

      Anti-mTOR Antibody, clone F11 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-mTOR Antibody, clone F11 Certificates of Analysis

      TitleLot Number
      Anti-mTOR, clone F11 - 2394072 2394072
      Anti-mTOR, clone F11 - 2433589 2433589
      Anti-mTOR, clone F11 - 2109557 2109557
      Anti-mTOR, clone F11 - 2279253 2279253
      Anti-mTOR, clone F11 - 3191458 3191458
      Anti-mTOR, clone F11 - 3490900 3490900
      Anti-mTOR, clone F11 - 3819968 3819968
      Anti-mTOR, clone F11 - NG1936908 NG1936908
      Anti-mTOR, clone F11 - NRG1817784 NRG1817784
      Anti-mTOR, clone F11 -2654346 2654346
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