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17-10101 ChIPAb+ Acetyl-Histone H4 (Lys16) - ChIP Validated Antibody and Primer Set

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17-10101
25 assays  25 assays per set. Recommended use: ~2 μL of antibody per chromatin immunoprecipitation (dependent upon biological context).
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey Applications
      H, VrtIP, WB, ChIP
      Description
      Catalogue Number17-10101
      Brand Family Upstate
      Trade Name
      • ChIPAb+
      • Upstate
      DescriptionChIPAb+ Acetyl-Histone H4 (Lys16) - ChIP Validated Antibody and Primer Set
      OverviewAll ChIPAb+ antibodies are individually validated for chromatin precipitation, every lot, every time. Each ChIPAb+ antibody set includes control primers (tested every lot by qPCR) to biologically validate your IP results in a locus-specific context. The qPCR protocol and primer sequences are provided, allowing researchers to validate ChIP protocols when using our antibody in their chromatin context. Each set also includes a negative control antibody to ensure specificity of the ChIP reaction.
      The ChIPAb+ Acetyl-Histone H4 (Lys16) set includes the Acetyl-Histone H4 (Lys16) antibody, a negative control normal rabbit IgG, and qPCR primers which amplify a 178 bp region of human RPL10 promoter. The Acetyl-Histone H4 (Lys16) and negative controls are supplied in a scalable "per ChIP" reaction size and can be used to functionally validate the precipitation of Acetyl-Histone H4 (Lys16)-associated chromatin.
      Alternate Names
      • H4K16Ac
      • Histone H4 (acetyl K16)
      • H4 histone family, member A
      • histone 1, H4a
      • histone cluster 1, H4a
      Background InformationHistone H4 is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H4 is involved with the structure of the nucleosomes of the 'beads on a string' structure. Acetylation of histone H4 occurs at several different lysine positions in the histone tail and is performed by a family of enzymes known as Histone Acetyl Transferases (HATs).
      References
      Product Information
      FormatPurified
      Control
      • Includes negative control normal rabbit IgG and primers specific for human RPL10 promoter.
      PresentationAnti-Acetyl-Histone H4 (Lys16) (rabbit polyclonal). One vial containing 50 µL of affinity purified rabbit polyclonal serum in buffer containing 0.1 M Tris-Glycine (pH 7.4) 150 mM NaCl with 0.05% sodium azide. Store at -20°C.

      Normal Rabbit IgG. One vial containing 125 µg of rabbit IgG in 125 µL of storage buffer containing 0.05% sodium azide. Store at -20°C.

      ChIP Primers, RPL10 promoter. One vial containing 75 μL of 5 μM of each primer specific for the promoter region of human RPL10. Store at -20°C.
      FOR: ACC CGT CTT CGA CAG GAC T
      REV: GGA ACG GAA GAC GAG AAC AG
      Quality LevelMQ100
      Applications
      ApplicationThis ChIPAb+ Acetyl-Histone H4 (Lys16) -ChIP Validated Antibody & Primer Set conveniently includes the antibody & the specific control PCR primers.
      Key Applications
      • Immunoprecipitation
      • Western Blotting
      • Chromatin Immunoprecipitation (ChIP)
      Application NotesChromatin Immunoprecipitation:
      Representative lot data.
      Sonicated chromatin prepared from HeLa cells (1 X 10E6 cell equivalents per IP) were subjected to chromatin immunoprecipitation using either 2 µg of Normal rabbit IgG or 2 µL of Anti-acetyl-Histone H4 (Lys16) and the Magna ChIP™ A Kit (Cat. # 17-610).
      Successful immunoprecipitation of acetyl-Histone H4 (Lys16) associated DNA fragments was verified by qPCR using ChIP Primers, RPL10 Promoter as a positive locus, and β-Globin primers as a negative locus. (Please see figures). Data is presented as percent input of each IP sample relative to input chromatin for each amplicon and ChIP sample as indicated.
      Please refer to the EZ-Magna ChIP™ A (Cat. # 17-408) or EZ-ChIP™ (Cat. # 17-371) protocol for experimental details.

      Western Blot Analysis:
      Representative lot data.
      Recombinant Histone H4 (Cat # 14-697) (lane1) and HeLa acid extract (lane 2) was resolved by electrophoresis, transferred to PVDF and probed with anti-Acetyl Histone H4 (Lys16) (0.1 μg/mL).
      Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.
      Arrow indicates Acetyl Histone H4 (Lys16) (~10 kDa) (Please see figures).

      Multiplexing (Luminex®):
      Representative lot data.
      This antibody specifically recognizes histone H4 acetylated on Lys16 by Luminex assay (Please see figures).

      Peptide Inhibition Assay:
      Representative lot data.
      This antibody peptide blocked on HeLa cell extracts (Please see figures).
      Biological Information
      ImmunogenKLH-conjugated Histone H4 corresponding to the N-terminus region at and around acetylated Lys16.
      EpitopeAcetylated H4 Lys16
      HostRabbit
      SpecificityThis antibody detects histone H4 acetylated at Lys16.
      Species Reactivity
      • Human
      • Vertebrates
      Species Reactivity NoteHuman. Broad species cross-reactivity is expected based on sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryHistones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6.
      Gene Symbol
      • HIST1H4A
      • H4/J
      • HIST1H4F
      • HIST1H4L
      • H4FN
      • H4FA
      • H4FH
      • HIST1H4H
      • H4F2
      • H4FM
      • H4/A
      • H4FK
      • H4FG
      • HIST2H4
      • H4FB
      • H4/K
      • HIST1H4I
      • H4/N
      • HIST1H4B
      • H4FE
      • H4/M
      • H4/a
      • HIST1H4E
      • HIST4H4
      • HIST2H4A
      • H4FC
      • H4FI
      • H4/H
      • HIST1H4C
      • H4/G
      • HIST1H4K
      • H4FJ
      • H4FD
      • H4/I
      • H4/B
      • H4/D
      • H4/C
      • HIST1H4J
      • HIST1H4D
      • H4/E
      Modifications
      • Acetylation
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P62805 # Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
      SIZE: 103 amino acids; 11367 Da
      SUBUNIT: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.
      SUBCELLULAR LOCATION: Nucleus.
      PTM: Symmetric dimethylation on Arg-4 by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage (By similarity). & Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins.
      SIMILARITY: SwissProt: P62805 ## Belongs to the histone H4 family.
      Molecular Weight~10 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceChromatin Immunoprecipitation:
      Sonicated chromatin prepared from HeLa cells (1 X 10E6 cell equivalents per IP) were subjected to chromatin immunoprecipitation using either 2 µg of Normal Rabbit IgG or 2 µL of Anti-acetyl-Histone H4 (Lys16) and the Magna ChIP™ A Kit (Cat. # 17-610).
      Successful immunoprecipitation of acetyl-Histone H4 (Lys16) associated DNA fragments was verified by qPCR using ChIP Primers, RPL10 Promoter (Please see figures).
      Please refer to the EZ-Magna ChIP™ A (Cat. # 17-408) or EZ-ChIP™ (Cat. # 17-371) protocol for experimental details.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size25 assays
      Material Package25 assays per set. Recommended use: ~2 μL of antibody per chromatin immunoprecipitation (dependent upon biological context).
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      17-10101 04053252480645

      Documentation

      ChIPAb+ Acetyl-Histone H4 (Lys16) - ChIP Validated Antibody and Primer Set Certificates of Analysis

      TitleLot Number
      ChIPAb+ Acetyl-Histone H4 (Lys16) - 2343603 2343603
      ChIPAb+ Acetyl-Histone H4 (Lys16) - 1771805B 1771805B
      ChIPAb+ Acetyl-Histone H4 (Lys16) - 1992663 1992663
      ChIPAb+ Acetyl-Histone H4 (Lys16) - 2137036 2137036
      ChIPAb+ Acetyl-Histone H4 (Lys16) - 2287792 2287792
      ChIPAb+ Acetyl-Histone H4 (Lys16) - 3780296 3780296
      ChIPAb+ Acetyl-Histone H4 (Lys16) - NRG1771805 NRG1771805
      ChIPAb+ Acetyl-Histone H4 (Lys16) - NRG1771805A NRG1771805A
      ChIPAb+ Acetyl-Histone H4 (Lys16) -2736572 2736572
      ChIPAb+ Acetyl-Histone H4 (Lys16) -3030898 3030898

      References

      Reference overviewPub Med ID
      Histone deacetylation of NIS promoter underlies BRAF V600E-promoted NIS silencing in thyroid cancer.
      Zhang, Z; Liu, D; Murugan, AK; Liu, Z; Xing, M
      Endocrine-related cancer  21  161-73  2014

      Show Abstract
      24243688 24243688
      Epigenetic suppression of mouse Per2 expression in the suprachiasmatic nucleus by the inhalational anesthetic, sevoflurane.
      Mori, K; Iijima, N; Higo, S; Aikawa, S; Matsuo, I; Takumi, K; Sakamoto, A; Ozawa, H
      PLoS One  9  e87319  2014

      Show Abstract
      24498074 24498074
      The histone acetyltransferase MOF activates hypothalamic polysialylation to prevent diet-induced obesity in mice.
      Brenachot, X; Rigault, C; Nédélec, E; Laderrière, A; Khanam, T; Gouazé, A; Chaudy, S; Lemoine, A; Datiche, F; Gascuel, J; Pénicaud, L; Benani, A
      Molecular metabolism  3  619-29  2014

      Show Abstract
      25161885 25161885
      Functional complementation of sir2Δ yeast mutation by the human orthologous gene SIRT1.
      Gaglio, D; D'Alfonso, A; Camilloni, G
      PloS one  8  e83114  2013

      Show Abstract
      24349441 24349441
      Plant 45S rDNA clusters are fragile sites and their instability is associated with epigenetic alterations.
      Huang, M; Li, H; Zhang, L; Gao, F; Wang, P; Hu, Y; Yan, S; Zhao, L; Zhang, Q; Tan, J; Liu, X; He, S; Li, L
      PloS one  7  e35139  2012

      Show Abstract
      22509394 22509394

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      Categories

      Life Science Research > Antibodies and Assays > Immunoassays > Immunoprecipitation (IP) > Chromatin Immunoprecipitation (ChIP) > ChIP Validated Antibodies
      Life Science Research > Antibodies and Assays > Primary Antibodies
      Life Science Research > Protein Detection and Quantification > Immunoassays > Immunoprecipitation (IP) > Chromatin Immunoprecipitation (ChIP) > ChIP Validated Antibodies