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17-10098 ChIPAb+ TATA Binding Protein (TBP) - ChIP Validated Antibody and Primer Set

The ChIPAb+ TATA Binding Protein (TBP) set includes the TATA Binding Protein (TBP) antibody, a negative control mouse ascites & qPCR primers which amplify a 166 bp region of human GAPDH promoter.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Western Blotting, Chromatin Immunoprecipitation (ChIP) Research Category: Epigenetics & Nuclear Function Research Sub-Category: Transcription Factors
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17-10098
25 assays  25 assays per set. Recommended use: ~2 μL of antibody per chromatin immunoprecipitation (dependent upon biological context).
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      Key Specifications Table

      Species ReactivityKey Applications
      Ch, H, M, Xn, FWB, ChIP
      Description
      Catalogue Number17-10098
      Brand Family Upstate
      Trade Name
      • ChIPAb+
      • Upstate
      DescriptionChIPAb+ TATA Binding Protein (TBP) - ChIP Validated Antibody and Primer Set
      OverviewAll ChIPAb+ antibodies are individually validated for chromatin precipitation, every lot, every time. Each ChIPAb+ antibody set includes control primers (tested every lot by qPCR) to biologically validate your IP results in a locus-specific context. The qPCR protocol and primer sequences are provided, allowing researchers to validate ChIP protocols when using our antibody in their chromatin context. Each set also includes a negative control antibody to ensure specificity of the ChIP reaction.
      The ChIPAb+ TATA Binding Protein (TBP) set includes the TATA Binding Protein (TBP) antibody, a negative control mouse ascites, and qPCR primers which amplify a 166 bp region of human GAPDH promoter. The TATA Binding Protein (TBP) and negative controls are supplied in a scalable "per ChIP" reaction size and can be used to functionally validate the precipitation of TATA Binding Protein (TBP)-associated chromatin.
      Alternate Names
      • TATA BINDING PROTEIN
      • TATA-box-binding protein
      • TATA-box factor
      • TATA-binding factor
      • TATA sequence-binding protein
      • Transcription initiation factor TFIID TBP subunit
      Background InformationTATA-box Binding Protein (TBP) is a general transcription factor that functions at the core of the DNA-binding multiprotein factor TFIID. Binding of TFIID to the TATA box is the initial transcriptional step of the pre-initiation complex (PIC). TBP plays a role in the activation of eukaryotic genes transcribed by RNA polymerase II and is a component of the transcription factor SL1/TIF-IB complex, which is involved in the assembly of the PIC (preinitiation complex) during RNA polymerase I-dependent transcription (Unitprot).
      References
      Product Information
      FormatAscites
      Control
      • Includes negative control mouse ascites and primers specific for human GAPDH promoter.
      PresentationAnti-TATA Binding Protein (TBP) (mouse monoclonal). One vial containing 50 μL ascites containing 0.05% sodium azide. Store at -20°C.

      Negative Ascites (mouse). One vial containing 50 µL of mouse ascites with 0.05% sodium azide. Store at -20°C.

      ChIP Primers, GAPDH promoter. One vial containing 75 μL of 5 μM each primer specific for human GAPDH. Store at -20°C.
      FOR: TAC TAG CGG TTT TAC GGG CG
      REV: TCG AAC AGG AGG AGC AGA GAG CGA
      Quality LevelMQ100
      Applications
      ApplicationThe ChIPAb+ TATA Binding Protein (TBP) set includes the TATA Binding Protein (TBP) antibody, a negative control mouse ascites & qPCR primers which amplify a 166 bp region of human GAPDH promoter.
      Key Applications
      • Western Blotting
      • Chromatin Immunoprecipitation (ChIP)
      Application NotesChromatin Immunoprecipitation:
      Sonicated chromatin prepared from HeLa cells (1 X 106 cell equivalents per IP) were subjected to chromatin immunoprecipitation using 2 µL of either Negative Ascites or 2 µL Anti-TATA Binding Protein (TBP) and the Magna ChIP™ G Kit (Cat. # 17-611).
      Successful immunoprecipitation of TBP associated DNA fragments was verified by qPCR using ChIP Primers, human GAPDH promoter as a positive locus, and GAPDH coding primers as a negative locus (Please see figures).
      Data is presented as percent input of each IP sample relative to input chromatin for each amplicon and ChIP sample as indicated.
      Please refer to the EZ-Magna ChIP™ G (Cat. # 17-409) or EZ-ChIP™ (Cat. # 17-371) protocol for experimental details.

      Western Blot Analysis:
      Representative lot data.
      HeLa lysates were resolved by electrophoresis, transferred to PVDF membrane and probed with Anti-TATA Binding Protein (TBP) (1:1000 dilution).
      Proteins were visualized using a secondary antibody conjugated to HRP and a chemiluminescence detection system (Please see figures).

      Immunohistochemistry:
      A 1:1,000-1:5,000 dilution of a previous lot was used in immunohistochemistry.

      Immunocytochemistry:
      A 1:1,000-1:5,000 dilution of a previous lot was used in immunocytochemistry.
      Biological Information
      ImmunogenFull length recombinant human TBP.
      HostMouse
      SpecificityRecognizes TATA Binding Protein (TBP).
      IsotypeIgG1
      Species Reactivity
      • Chicken
      • Human
      • Mouse
      • Xenopus
      • Fish
      Species Reactivity NoteHuman, mouse, chicken, xenopus and zebrafish. Does not react with Drososphila.
      Reactivity with other species has not been confirmed.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryInitiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes TBP, the TATA-binding protein. A distinctive feature of TBP is a long string of glutamines in the N-terminal. This region of the protein modulates the DNA binding activity of the C terminus, and modulation of DNA binding affects the rate of transcription complex formation and initiation of transcription. Mutations that expand the number of CAG repeats encoding this polyglutamine tract, and thus increase the length of the polyglutamine string, are associated with spinocerebellar ataxia 17, a neurodegenerative disorder classified as a polyglutamine disease.
      Gene Symbol
      • TBP
      • MGC126055
      • SCA17
      • GTF2D1
      • TFIID
      • TF2D
      • MGC117320
      • GTF2D
      • MGC126054
      Non-Reactive Species
      • Drosophila
      Purification MethodUnpurified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P20226 # General transcription factor that functions at the core of the DNA-binding multiprotein factor TFIID. Binding of TFIID to the TATA box is the initial transcriptional step of the pre- initiation complex (PIC), playing a role in the activation of eukaryotic genes transcribed by RNA polymerase II.
      SIZE: 339 amino acids; 37698 Da
      SUBUNIT: Belongs to the TFIID complex together with the TBP- associated factors (TAFs). Component of the transcription factor SL1/TIFIB complex, composed of TBP and at least TAF1A, TAF1B TAF1C, and TAF3. Binds DNA as monomer. Interacts with TAFs, TFIIB, NCOA6, DRAP1, DR1 and ELF3. Interacts with SPIB, SNAPC1, SNAPC2 and SNAPC4. Interacts with HIV-1 Tat. Interacts with UTF1 which acts as a coactivator of ATF2 transcriptional activity.
      SUBCELLULAR LOCATION: Nucleus.
      DISEASE: SwissProt: P20226 # Defects in TBP are the cause of spinocerebellar ataxia 17 (SCA17) [MIM:607136]. Spinocerebellar ataxia is a clinically and genetically heterogeneous group of autosomal dominant cerebellar ataxias (ADCA). Patients show progressive incoordination of gait and often poor coordination of hands, speech and eye movements. Spinocerebellar ataxia is caused by degeneration of the cerebellum with variable involvement of the brainstem and spinal cord. The molecular defect in SCA17 is the expansion of a CAG repeat in the coding region of TBP. Longer expansions result in earlier onset and more severe clinical manifestations of the disease.
      SIMILARITY: SwissProt: P20226 ## Belongs to the TBP family.
      Molecular Weight~42 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceChromatin Immunoprecipitation:
      Sonicated chromatin prepared from HeLa cells (1 X 106 cell equivalents per IP) were subjected to chromatin immunoprecipitation using 2 µL of either Negative Ascites or 2 µL Anti-TATA Binding Protein (TBP) and the Magna ChIP™ G Kit (Cat. # 17-611).
      Successful immunoprecipitation of TATA Binding Protein (TBP)-associated DNA fragments was verified by qPCR using ChIP Primers, human GAPDH promoter (Please see figures).

      Please refer to the EZ-Magna ChIP™ G (Cat. # 17-409) or EZ-ChIP™ (Cat. # 17-371) protocol for experimental details.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size25 assays
      Material Package25 assays per set. Recommended use: ~2 μL of antibody per chromatin immunoprecipitation (dependent upon biological context).
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      17-10098 04053252552854

      Documentation

      ChIPAb+ TATA Binding Protein (TBP) - ChIP Validated Antibody and Primer Set SDS

      Title

      Safety Data Sheet (SDS) 

      ChIPAb+ TATA Binding Protein (TBP) - ChIP Validated Antibody and Primer Set Certificates of Analysis

      TitleLot Number
      ChIPAb+ TATA Binding Protein (TBP) - 2064318 2064318
      ChIPAb+ TATA Binding Protein (TBP) - 3464623 3464623
      ChIPAb+ TATA Binding Protein (TBP) - NG1915320 NG1915320
      ChIPAb+ TATA Binding Protein (TBP) - NRG1755672 NRG1755672
      ChIPAb+ TATA Binding Protein (TBP) -2632092 2632092
      ChIPAb+ TATA Binding Protein (TBP) -2682400 2682400
      ChIPAb+ TATA Binding Protein (TBP) Monoclonal Antibody/Primer Set 3011403
      ChIPAb+ TATA Binding Protein (TBP) Monoclonal Antibody/Primer Set 3144138

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      Categories

      Life Science Research > Antibodies and Assays > Immunoassays > Immunoprecipitation (IP) > Chromatin Immunoprecipitation (ChIP) > ChIP Validated Antibodies
      Life Science Research > Antibodies and Assays > Primary Antibodies
      Life Science Research > Protein Detection and Quantification > Immunoassays > Immunoprecipitation (IP) > Chromatin Immunoprecipitation (ChIP) > ChIP Validated Antibodies