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HTS016M ChemiSCREEN™ C3aR Membrane Preparation

Human C3aR GPCR membrane preparation for Radioligand binding Assays.

ChemiSCREEN™ C3aR Membrane Preparation MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Host Cell: Chem-2 Uni Prot Number: Q16581 Entrez Gene Number: NM_004054.2
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HTS016M
1 mL  
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      Overview

      Replacement Information

      Key Specifications Table

      SpeciesHost CellsProtein Target
      Human Chem-2C3aR
      Description
      Catalogue NumberHTS016M
      Brand Family Chemicon®
      Trade Name
      • ChemiScreen
      • Chemicon
      DescriptionChemiSCREEN™ C3aR Membrane Preparation
      OverviewFull-length human C3aR cDNA
      Background InformationC3a, along with C4a and C5a, is a 77 amino acid anaphylotoxin generated by proteolytic cleavage during activation of the complement pathway (Ember, 1997). The anaphylotoxins strongly promote inflammation by recruiting leukocytes, particularly basophils, eosinophils, neutrophils and monocyte (Martin, 1997). The proinflammatory properties of C3a are mediated by interaction between the peptide and a 7-TM G-protein coupled receptor, C3aR (Crass, 1996). Genetic and pharmacological inhibition of C3a/C3aR interaction indicates an important role for C3aR in allergic asthsma Humbles, 2000; Drouin, 2002). C3a/C3aR also enhances the effect of SDF-1 in promoting retention of haematopoietic stem/progenitor cells within the bone marrow (Rataiczak, 2004). Chemicon's C3aR membrane preps are an ideal tool for screening for compounds that disrupt binding of C3a to C3aR. The membranes have been shown to bind to 125I-labeled C3a with a Kd of 0.06 nM.
      References
      Product Information
      FormatMembranes
      PresentationMembranes were adjusted to 0.5mg protein/mL in 50 mM Tris-HCL, pH 7.4, 10% glycerol and 1% BSA, rapidly frozen, and stored at -80°C. One vial contains 500 units, where a unit is the amount of membrane that will yield greater than 1000 cpm specific binding of 125I-labeled C3a (0.04 nM input concentration).
      Quality LevelMQ100
      Applications
      ApplicationHuman C3aR GPCR membrane preparation for Radioligand binding Assays.
      Key Applications
      • Radioligand binding assay
      Application NotesRadioligand binding assay
      Biological Information
      Concentration0.5mg/ml
      Entrez Gene Number
      Gene Symbol
      • C3AR1
      • C3a-R
      • HNFAG09
      • C3AR
      • C3R1
      • AZ3B
      GPCR ClassA
      Host CellsChem-2
      Protein TargetC3aR
      Target Sub-FamilyAnaphylotoxin
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q16581 # Receptor for the chemotactic and inflammatory peptide anaphylatoxin C3a. This receptor stimulates chemotaxis, granule enzyme release and superoxide anion production.
      SIZE: 482 amino acids; 53864 Da
      SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
      TISSUE SPECIFICITY: Widely expressed in several differentiated hematopoietic cell lines, in the lung, spleen, ovary, placenta, small intestine, throughout the brain, heart, and endothelial cells. Mostly expressed in lymphoid tissues.
      PTM: Among the sulfation sites Tyr-174 is essential for binding of C3a anaphylatoxin.
      SIMILARITY: SwissProt: Q16581 ## Belongs to the G-protein coupled receptor 1 family.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceBmax for [125I]C3a binding: 1.9 pmol/mg protein
      Kd for [125I] C3a binding: ~ 0.06 nM
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain frozen at -70°C for up to 2 years. Do not freeze and thaw.
      Packaging Information
      Material Size1 mL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      HTS016M 04053252409509

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