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SCR103 Collagenase type I

The collagenase type I (from Clostridium histolyticum) is a crude collagenase preparation that can be used for the isolation of primary cells or for tissue dissociation by enzymatic means.

Collagenase type I MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Stem Cell Culture
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SCR103
250 mg  1 vial containing 250mg of lyophilized powder
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      Overview

      Replacement Information

      Key Specifications Table

      Key Applications
      Cult
      Description
      Catalogue NumberSCR103
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionCollagenase type I
      OverviewCollagenase type I (from Clostridium histolyticum) is a crude collagenase preparation that can be used for the isolation of primary cells or for tissue dissociation by enzymatic means. The preparation also may contain caseinase, clostripain, and tryptic activities. This preparation can be used to dissociate cells in culture, and has been successfully used to passage human embryonic stem cells cultured with Millipore’s HEScGROTM Animal Component-Free Medium.
      Materials Required but Not Delivered• Ultrapure water (e.g., MilliQ)
      • 0.22 μM sterile filtration device (e.g. Millipore’s Stericup-GP, Catalog # SCGPU01RE)
      References
      Product Information
      HS Code3507 90 19
      Formulation
      Quality LevelMQ100
      Applications
      ApplicationThe collagenase type I (from Clostridium histolyticum) is a crude collagenase preparation that can be used for the isolation of primary cells or for tissue dissociation by enzymatic means.
      Key Applications
      • Stem Cell Culture
      Biological Information
      Media FormLiquid
      Stem Cell Type
      • Human Embryonic Stem Cells
      • Induced Pluripotent Stem Cells
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEach lot of collagenase type I is assayed via a modification of Mandl (1964), with the extent of collagen breakdown determined using the colorimetric ninhydrin method of Moore and Stein (1948). Each lot is then tested for successful passage of human embryonic stem cells cultured in Millipore's HEScGRO Medium for hES cells. Cells are characterized for correct marker expression and morphology after five passages.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsCollagenase type I should be stored in its powdered form at 2-8oC until ready to use. Solutions of collagenase type I can be aliquotted and stored at -20oC, but should not be repeatedly thawed and re-frozen.
      Packaging Information
      Material Size250 mg
      Material Package1 vial containing 250mg of lyophilized powder
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      SCR103 04053252397868

      Documentation

      Collagenase type I SDS

      Title

      Safety Data Sheet (SDS) 

      Collagenase type I Certificates of Analysis

      TitleLot Number
      Collagenase type I 3079679
      Collagenase type I 2978576
      Collagenase type I 3034146
      Collagenase type I 3130613
      Collagenase type I 3022083
      Collagenase type I 3049470
      Collagenase type I 2909011
      Collagenase type I 3003794
      Collagenase type I 3099012
      Collagenase type I -
      Show more

      Technical Info

      Title
      Collagenase Type I for Enzymatic Passaging of Human Embryonic Stem Cells in HEScGRO Medium

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      Categories

      Life Science Research > Cell Culture and Systems > Cell Culture Media and Supplements > Stem Cell Culture Media