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S7802 CpG WIZ® p15 -Methylation specific PCR assay

The CpG WIZ p15 Amplification Kit is used for determining the methylation status of the p15 promoter by methylation-specific PCR (MSP).

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S7802
25 assays  
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      Overview

      Replacement Information
      Description
      Catalogue NumberS7802
      Brand Family Chemicon®
      Trade Name
      • CpG Wiz
      • Chemicon
      DescriptionCpG WIZ® p15 -Methylation specific PCR assay
      OverviewAberrant methylation of the p15 promoter region has been shown to cause inactivation in several gliomas and leukemias. The CpG WIZ® p15 Amplification Kit is used for determining the methylation status of the p15 promoter by methylation-specific PCR (MSP). The kit contains primers targeted to regions of the p15 promoter where the sequences are most divergent after bisulfite treatment. PCR parameters have been identified so that all primer sets in the kit amplify template under the same conditions. Control genomic DNA samples (methylated and unmethylated) for p15* are also included.



      Methylation-specific PCR (MSP) is a new technology for sensitive detection of abnormal gene methylation utilizing small amounts of DNA (Herman, 1996 ). This process employs an initial bisulfite reaction to modify the DNA, followed by PCR amplification with specific primers designed to distinguish methylated from unmethylated DNA.



      *p15 is covered under U.S. patents 5,994,095, US 6,140,473, 6,180,776, 6,210,949 and 6,218,146.
      References
      Product Information
      Quality LevelMQ100
      Applications
      ApplicationThe CpG WIZ p15 Amplification Kit is used for determining the methylation status of the p15 promoter by methylation-specific PCR (MSP).
      Key Applications
      • PCR
      Application NotesPrinciples of the Technique

      Use of either the CpGenome™ DNA Modification Kit (Cat. No. S7820) or the CpGENOME™ Fast DNA Modification Kit (Cat. No. S7824) facilitates the initial bisulfite reactions, while the The CpG WIZ® p15 Amplification Kit contains the reagents required for the gene-specific PCR amplification reactions.
      Biological Information
      Species Reactivity
      • Human
      Entrez Gene Number
      Entrez Gene SummaryThis gene lies adjacent to the tumor suppressor gene CDKN2A in a region that is frequently mutated and deleted in a wide variety of tumors. This gene encodes a cyclin-dependent kinase inhibitor, which forms a complex with CDK4 or CDK6, and prevents the activation of the CDK kinases, thus the encoded protein functions as a cell growth regulator that controls cell cycle G1 progression. The expression of this gene was found to be dramatically induced by TGF beta, which suggested its role in the TGF beta induced growth inhibition. Two alternatively spliced transcript variants of this gene, which encode distinct proteins, have been reported.
      Gene Symbol
      • CDKN2B
      • TP15
      • INK4B
      • p15INK4b
      • p14-INK4b
      • P15
      • MTS2
      • p15-INK4b
      • MTS-2
      • p14_INK4B
      • p15INK4B
      • CDK4I
      • p15_INK4B
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P42772 # Interacts strongly with CDK4 and CDK6. Potent inhibitor. Potential effector of TGF-beta induced cell cycle arrest.
      SIZE: 138 amino acids; 14722 Da
      SUBUNIT: Heterodimer of CDKN2B with CDK4 or CDK6.
      DISEASE:SwissProt: P42772 # Defects in CDKN2B are involved in tumor formation.
      SIMILARITY: SwissProt: P42772 ## Belongs to the CDKN2 cyclin-dependent kinase inhibitor family. & Contains 4 ANK repeats.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Packaging Information
      Material Size25 assays
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      S7802 04053252726255

      Documentation