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SCM017 ENStem-A™ Neuronal Differentiation Medium

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SCM017
100 mL  
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      Overview

      Replacement Information

      Key Specifications Table

      Key Applications
      Cult
      Description
      Catalogue NumberSCM017
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionENStem-A™ Neuronal Differentiation Medium
      OverviewENStem-A™ Neuronal Differentiation Medium is a specially formulated medium optimized for the preferential differentiation of ENStem-A™ human neural progenitor cells to a neuronal lineage. The medium has been extensively validated on ENStem-A™ Human Neural Progenitor Cells. Using ENStem-A™ Neuronal Differentiation Medium, relatively pure populations of neuronal cells (>90% bIII-tubulin positive) are obtained. Very low levels of astrocytes (<0.5% GFAP positive) are detected. Sufficient medium is supplied to provide for 100 separate reactions at 1 mL volume or 10 reactions at 10 mL volume.
      Materials Required but Not Delivered• L-Glutamine (Cat. No. TMS-002-C)
      • ENStem-A™ Human Neural Progenitor Cells Kit (Cat. No. SCR055)
      • ENStem-A™ Neural Expansion Medium (Cat. No. SCM004)
      • ENStem-A™ Neural Freezing Medium (Cat. No. SCM011)
      • Human Neural Stem Cell Characterization Kit (Cat. No. SCR060)
      • Poly-L-ornithine (Sigma Part No. P3655)
      • Laminin (Cat. No. CC095)
      • Penicillin/Streptomycin Solution (Cat. No. TMS-AB2-C)
      • Accutase™ (Cat. No. SCR005)
      • Chamber slides
      • Tissue culture-ware
      • Phosphate-Buffered Saline (1X PBS) (Cat. No. BSS-1005-B)
      • Hemocytometer
      • Microscope
      References
      Product Information
      Quality LevelMQ100
      Applications
      ApplicationThe ENStem-A Neuronal Differentiation Medium is a specially formulated medium optimized for the preferential differentiation of ENStem-A human neural progenitor cells to a neuronal lineage.
      Key Applications
      • Stem Cell Culture
      Application NotesCell culture protocols for use with ENStem-A™ Human Neural Progenitor Cells are available in the datasheets that accompany the ENStem-A™ Human Neural Progenitor Expansion Kit (Part No. SCR055).
      Biological Information
      Media FormLiquid
      Stem Cell Type
      • Neural Stem Cells
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEach lot of media is tested for the optimal differentiation of ENStem-A™ Human Neural Progenitor Cells to a neuronal lineage.
      Sterility Testing: Negative
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsENStem-A™ Neuronal Differentiation Medium should be stored at -20°C until ready to use. Upon thawing, fresh L-Glutamine (not provided) should be added for a final concentration of 2 mM to the medium. Thawed medium should be stored at 2-8°C and given a 1-month expiration dating. Dispense into aliquots to avoid repeated heating prior to each use.
      Packaging Information
      Material Size100 mL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      SCM017 04053252724527