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SCC112 FLAG-SNAP-hTERT expressing HeLa Cell Line

FLAG-SNAP-hTERT expressing HeLa Cell Line is a useful tool for studying telomerase biology and enables purification and visualization of endogenous telomerase reverse transcriptase (TERT).

FLAG-SNAP-hTERT expressing HeLa Cell Line MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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SCC112
≥1X10⁶ cells/vial  
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      Overview

      Replacement Information
      Description
      Catalogue NumberSCC112
      DescriptionFLAG-SNAP-hTERT expressing HeLa Cell Line
      OverviewTo facilitate reliable detection of the endogenous TERT protein, a FLAG-SNAP tag was fused to the N-terminus of the TERT locus in HeLa cells. The FLAG-SNAP tag does not impair the function of the endogenous hTERT protein. Efficient immunopurification (IP) of the active telomerase ribonucleoprotein complexes may be accomplished by using the well-characterized FLAG antibody. The SNAP tag may be used to visualize subcellular localization of the low abundant endogenous TERT protein.

      Reference:
      Xi L, Schmidt JC, Zaug AJ, Ascarrunz DR, and Cech TR (2016) A novel two-step genome editing strategy with CRISPR-Cas9 provides new insights into telomerase action and TERT gene expression. Genome Biology 16: 231-248.
      Background InformationTelomerase play a critical role in regulating proliferation and senescence in normal somatic cells as well as most cancer cells. In humans, the telomerase reverse transcriptase (hTERT) is a lowly expressed protein with only several hundred molecules per cell. The low expression has made it difficult to detect, purify and study TERT activity from the endogenous loci.
      References
      Product Information
      Quality LevelMQ100
      Applications
      ApplicationFLAG-SNAP-hTERT expressing HeLa Cell Line is a useful tool for studying telomerase biology and enables purification and visualization of endogenous telomerase reverse transcriptase (TERT).
      Application NotesThis product is intended for sale and sold solely to academic institutions for internal academic research use per the terms of the “Academic Use Agreement” as detailed in the product documentation. For information regarding any other use, please contact licensing@emdmillipore.com.
      Biological Information
      Cell Line Type
      • Cancer Cells
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assurance• Each vial contains ≥1X10⁶ viable cells.
      • Cells are tested negative for HPV-16, Hepatitis A, B, C, and HIV-1 & 2 viruses by PCR.
      • Cells are negative for mycoplasma contamination.
      • Cells are positive for HPV-18 by PCR.
      • Each lot of cells is genotyped by STR analysis to verify the unique identity of the cell line.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      • This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges MilliporeSigma to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
      Storage and Shipping Information
      Packaging Information
      Material Size≥1X10⁶ cells/vial
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      SCC112 04054839226960