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PF033 Fas Ligand, Human, Recombinant

Fas Ligand, Human, Recombinant, consists of amino acids 103-281 fused at the N-terminus to a tag-linker peptide. Binds to human, rat, and mouse Fas.

Fas Ligand, Human, Recombinant MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: CD95L, CD178

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PF033
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Overview

Replacement Information

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PF033-10UG
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      		 Plastic ampoule 10 μg
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      Description
      OverviewRecombinant, human, Fas ligand consisting of amino acids 103-281 fused at the N-terminus to a tag-linker peptide. Binds to human, rat, and mouse Fas. The recombinant human soluble Fas ligand recognizes the Fas receptor in human, rat and mouse. Extracellular domain of human FasL, 103 - 281 fused at the N-terminus to a tag linker peptide.
      Catalogue NumberPF033
      Brand Family Calbiochem®
      SynonymsCD95L, CD178
      References
      ReferencesNoguchi, K. et al. 1996. Oncogene 13, 39.
      Golstein, P. et al. 1995. Cell 81, 185.
      Katsikis, P. et al. 1995. J. Exp. Med. 181, 2029.
      Nagata, S. et al. 1995. Science 267, 1449.
      Thompson, C., 1995. Science 267, 1456.
      Watanabe-Fukunaga, R. et al. 1992. Nature 356, 314.
      Product Information
      ActivityED50:50 ng/ml (A20 cells)
      FormLyophilized
      FormulationLyophilized from PBS.
      PreservativeNone
      Quality LevelMQ100
      Applications
      Application CommentsThe soluble Fas ligand kills APO-1/Fas-sensitive cells at a concentration of >50 ng/ml. For example, cell death results when 5 x 105 murine A20 B lymphoma cells are incubated with 50-500 ng/ml of the recombinant human soluble APO1/FasL (aa 103-281) for 16 h at 37°C.
      Biological Information
      Purity≥95% by SDS-PAGE
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      ContaminantsEndotoxin: <0.1 EU/µg purified protein
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      PF033-10UG 04055977207989

      Documentation

      Fas Ligand, Human, Recombinant SDS

      Title

      Safety Data Sheet (SDS) 

      Fas Ligand, Human, Recombinant Certificates of Analysis

      TitleLot Number
      PF033

      References

      Reference overview
      Noguchi, K. et al. 1996. Oncogene 13, 39.
      Golstein, P. et al. 1995. Cell 81, 185.
      Katsikis, P. et al. 1995. J. Exp. Med. 181, 2029.
      Nagata, S. et al. 1995. Science 267, 1449.
      Thompson, C., 1995. Science 267, 1456.
      Watanabe-Fukunaga, R. et al. 1992. Nature 356, 314.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision16-September-2008 RFH
      SynonymsCD95L, CD178
      DescriptionRecombinant, human, Fas ligand consisting of amino acids 103-281 fused at the N-terminus to a tag-linker peptide. Binds to human, mouse, and rat Fas. The recombinant protein is produced in HEK293 cells. Useful for cytotoxicity assays.

      Fas/APO1/CD95 (37-42 kDa), a member of the Tumor Necrosis Factor/Nerve Growth Factor (TNF/NGF) receptor family, is an apoptosis-signaling surface receptor known to trigger apoptotic cell death. Homology exists between the Fas and TNF receptors including a region called the "death domain" which is required to propagate the apoptotic signal. The signal activates a family of cysteine proteases, or caspases, which systematically lead to cell destruction. Genetic defects in the Fas system have been shown to lead to autoimmune disorders while increased activity or deregulation contributes to the pathology of diseases such as AIDS.
      BackgroundFas/APO1/CD95 (36 kDa), a member of the Tumor Necrosis Factor/Nerve Growth Factor (TNF/NGF) receptor family, is an apoptosis-signaling surface receptor known to trigger apoptotic cell death. Homology exists between the Fas and TNF receptors including a region called the "death domain" which is required to propagate the apoptotic signal. The signal activates a family of cysteine proteases, or caspases, which systematically lead to cell destruction. Genetic defects in the Fas system have been shown to lead to autoimmune disorders while increased activity or deregulation contributes to the pathology of diseases such as AIDS.
      Specieshuman, mouse, rat
      FormLyophilized
      FormulationLyophilized from PBS.
      Concentration Label Please refer to vial label for lot-specific concentration
      Recommended reaction conditionsSoluble Fas ligand kills APO-1/Fas-sensitive cells at a concentration of >50 ng/ml. For example, cell death results when 5 x 105 murine A20 B lymphoma cells are incubated with 50-500 ng/ml recombinant human soluble APO1/FasL for 16 h at 37°C.
      Purity≥95% by SDS-PAGE
      ContaminantsEndotoxin: <0.1 EU/µg purified protein
      ActivityED50:50 ng/ml (A20 cells)
      SolubilityReconstitute in 50 μl H₂O to yield a final stock concentration of 100 µg/ml. Further dilute in culture medium containing 5% FCS immediately prior to use.
      PreservativeNone
      CommentsThe soluble Fas ligand kills APO-1/Fas-sensitive cells at a concentration of >50 ng/ml. For example, cell death results when 5 x 105 murine A20 B lymphoma cells are incubated with 50-500 ng/ml of the recombinant human soluble APO1/FasL (aa 103-281) for 16 h at 37°C.
      Storage -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
      Toxicity Standard Handling
      ReferencesNoguchi, K. et al. 1996. Oncogene 13, 39.
      Golstein, P. et al. 1995. Cell 81, 185.
      Katsikis, P. et al. 1995. J. Exp. Med. 181, 2029.
      Nagata, S. et al. 1995. Science 267, 1449.
      Thompson, C., 1995. Science 267, 1456.
      Watanabe-Fukunaga, R. et al. 1992. Nature 356, 314.