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SCT151 Firefly Luciferase Assay (Lyophilized)

Lyophilized flash-type luciferase assay designed for simple and efficient quantitation of firefly luciferase reporter enzyme activity from cultured cells with high sensitivity and linearity.

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SCT151
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      Overview

      Replacement Information
      Description
      Catalogue NumberSCT151
      DescriptionFirefly Luciferase Assay (Lyophilized)
      OverviewFirefly luciferase is widely used as a reporter for studying gene regulation and function, and for pharmaceutical screening. It is a very sensitive genetic reporter due to the absence of endogenous luciferase activity in mammalian cells or tissues. Firefly luciferase is a 62,000 Dalton protein, which is active as a monomer and does not require subsequent processing for its activity. The enzyme catalyzes ATP-dependent D-luciferin oxidation to oxyluciferin, producing light emission centered at 560 nm. Light emitted from the reaction is directly proportional to the number of luciferase enzyme molecules.

      The Firefly Luciferase Assay (Lyophilized) is designed for simple and efficient quantitation of firefly luciferase reporter enzyme activity from cultured cells with high sensitivity and linearity. The Firefly Assay buffer is packaged as a convenient lyophilized powder, which can be shipped at ambient temperature and stored at –20 °C instead of –80 °C. This is a flash-type luminescence assay with signal half-life of about 12 minutes.
      Alternate Names
      • Firefly Luciferase Assay
      • Bioluminescent Assay
      References
      Product Information
      Components
      • 1) 5X Firefly Luciferase Lysis Buffer (CS224525): 2 X 15 mL
      • 2) Firefly Luciferase Assay Buffer (Lyophilized) (CS224526): 1000 assays
      • 3) D-Luciferin (CS224519): 2 X 10 mg
      Detection methodChemiluminescence
      HS Code3822 19 90
      PresentationLiquid
      Applications
      ApplicationLyophilized flash-type luciferase assay designed for simple and efficient quantitation of firefly luciferase reporter enzyme activity from cultured cells with high sensitivity and linearity.
      Key Applications
      • Cell Based Assays
      Application NotesFirefly Luciferase Assay
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assurance.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore Firefly Luciferase Assay (Lyophilized) at -20°C. The kit is stable at -20°C for at least six months from date of receipt. After reconstitution, aliquot assay buffer if necessary to avoid repeated freeze-thaw cycles; reconstituted assay buffer is stable at -20°C for at least 3 months or -80°C for at least 6 months. Firefly luciferase working solution (assay buffer + D-luciferin) should be prepared fresh on the day of assay.
      Packaging Information
      Material Size1 kit
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      SCT151 04054839566035