Millipore Sigma Vibrant Logo

109204 Giemsa's azur eosin methylene blue solution

View Products on Sigmaaldrich.com
109204
View Pricing & Availability

Overview

Replacement Information

Pricing & Availability

Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
1092040100
Retrieving availability...
Limited Availability
Limited Availability
Stocked 
Discontinued
Limited Quantities Available
Available
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service

      Glass bottle 100 ml
      Retrieving price...
      Price could not be retrieved
      Minimum Quantity is a multiple of
      Maximum Quantity is
      Upon Order Completion More Information
      You Saved ()
       
      Request Pricing
      1092040500
      Retrieving availability...
      Limited Availability
Limited Availability
      Stocked 
      Discontinued
      Limited Quantities Available
      Available
        Remaining : Will advise
          Remaining : Will advise
          Will advise
          Contact Customer Service
          Contact Customer Service

          Plastic bottle 500 ml
          Retrieving price...
          Price could not be retrieved
          Minimum Quantity is a multiple of
          Maximum Quantity is
          Upon Order Completion More Information
          You Saved ()
           
          Request Pricing
          1092041000
          Retrieving availability...
          Limited Availability
Limited Availability
          Stocked 
          Discontinued
          Limited Quantities Available
          Available
            Remaining : Will advise
              Remaining : Will advise
              Will advise
              Contact Customer Service
              Contact Customer Service

              Plastic bottle 1 l
              Retrieving price...
              Price could not be retrieved
              Minimum Quantity is a multiple of
              Maximum Quantity is
              Upon Order Completion More Information
              You Saved ()
               
              Request Pricing
              1092042500
              Retrieving availability...
              Limited Availability
Limited Availability
              Stocked 
              Discontinued
              Limited Quantities Available
              Available
                Remaining : Will advise
                  Remaining : Will advise
                  Will advise
                  Contact Customer Service
                  Contact Customer Service

                  Plastic bottle 2.5 l
                  Retrieving price...
                  Price could not be retrieved
                  Minimum Quantity is a multiple of
                  Maximum Quantity is
                  Upon Order Completion More Information
                  You Saved ()
                   
                  Request Pricing
                  1092049025
                  Retrieving availability...
                  Limited Availability
Limited Availability
                  Stocked 
                  Discontinued
                  Limited Quantities Available
                  Available
                    Remaining : Will advise
                      Remaining : Will advise
                      Will advise
                      Contact Customer Service
                      Contact Customer Service

                      Barrel PE/met 25 l
                      Retrieving price...
                      Price could not be retrieved
                      Minimum Quantity is a multiple of
                      Maximum Quantity is
                      Upon Order Completion More Information
                      You Saved ()
                       
                      Request Pricing
                      Description
                      Catalogue Number109204
                      DescriptionGiemsa's azur-eosin-methylene blue solution
                      OverviewGiemsa’s Azure Eosin Methylene Blue solution - for microscopy, can be used for the staining of blood and bone marrow smears, paraffin sections and clinical-cytological specimens. Giemsa staining is one of the standard procedures in histology, demonstrating various cells with their morphological features better than with H&E stain. When used in hematological applications, Giemsa’s stain is frequently used in combination with other staining solutions, e. g. with May-Grünwald’s solution for Pappenheim (MGG) overview staining. This ready-to-use staining solution generally stains the nuclei red, based on the molecular interaction between the eosin Y dye and an Azure B-DNA complex. Both dyes assemble to an Eosin Y - Azure B-DNA complex and the intensity of the resulting stain depends on the content of Azure B and the ratio of Azure B : Eosin Y. Furthermore, the resulting stain can vary depending on the influence of fixation, staining times, pH-value of the solutions or buffer substances. In histology and clinico-cytological applications, Giemsa’s staining without additional stainings is used as an extended overview staining method. In this method, the color of the various cell components is influenced by pretreatment of the specimen material. Here, chromatin-containing structures (e. g. cell nuclei) appear in various blue shades, while the acidophilic components show up in a variety of red shades. The 500 ml bottle is sufficient for 3500-5000 stainings. This product is registered as IVD and CE marked. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.
                      References
                      Product Information
                      HS Code3212 90 90
                      Quality LevelMQ400
                      Applications
                      Applicationfor microscopy
                      Biological Information
                      Physicochemical Information
                      Boiling point>65 °C (1013 hPa)
                      Density0.99 g/cm3 (20 °C)
                      Explosion limit5.5 - 44 %(V) Methanol
                      Flash point18 °C
                      Ignition temperature455 °C
                      pH value6.0 - 8.2 (H₂O, 20 °C) (undiluted)
                      Dimensions
                      Materials Information
                      Toxicological Information
                      Safety Information according to GHS
                      Hazard Pictogram(s) Hazard Pictogram(s)
                      Hazard Pictogram(s)
                      Hazard Pictogram(s)
                      Hazard Statement(s)H225: Highly flammable liquid and vapour.
                      H301 + H311 + H331: Toxic if swallowed, in contact with skin or if inhaled.
                      H317: May cause an allergic skin reaction.
                      H370: Causes damage to organs.
                      Precautionary Statement(s)P210: Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
                      P233: Keep container tightly closed.
                      P280: Wear protective gloves/ protective clothing/ eye protection/ face protection/ hearing protection.
                      P301 + P310: IF SWALLOWED: Immediately call a POISON CENTER/doctor.
                      P303 + P361 + P353: IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water.
                      P304 + P340 + P311: IF INHALED: Remove person to fresh air and keep comfortable for breathing. Call a POISON CENTER/doctor.
                      P403 + P233: Store in a well-ventilated place. Keep container tightly closed.
                      Signal WordDanger
                      Storage class3 Flammable liquids
                      WGKWGK 2 obviously hazardous to water
                      Disposal9
                      Carcinogenic compounds and flammable compounds labelled "Highly toxic" or "Toxic": container F. Alkyl sulfates are carcinogenic; Take particular care to avoid inhalation and skin contact. To neutralize alkyl sulfates, add dropwise (from a dropping funnel) to concentrated ice-cool Ammonia solution (Cat.No. 105426) with vigorous stirring. Before placing in container D, check the pH with pH Universal indicator strips (Cat.No. 109535).
                      Safety Information
                      Categories of dangerhighly flammable, toxic
                      Product Usage Statements
                      Storage and Shipping Information
                      StorageStore at +15°C to +25°C.
                      Packaging Information
                      Transport Information
                      Declaration (railroad and road) ADR, RIDUN 1230 , 3 (6.1), II
                      Declaration (transport by air) IATA-DGRUN 1230 , 3 (6.1), II
                      Declaration (transport by sea) IMDG-CodeUN 1230 , 3 (6.1), II
                      Supplemental Information
                      Specifications
                      Suitability for microscopypasses test
                      Erythrocytesreddish-brownish
                      Nuclei / Chromatinred to violet
                      Eosinophilic granulesreddish to red-brown
                      Neutrophilic granuleslight violet
                      Lymphocyte cytoplasmnblue
                      Monocyte cytoplasmgrey-blue
                      Thrombocytesviolet
                      Basophilic granulesdark violet
                      Nuclei (Tissue section)blue to dark blue
                      Cellsblue to dark blue
                      Collagenpale blue
                      Osteoidpale blue
                      Eosinophilic granula (Tissue section)red
                      Acidic mucopolysaccharidesreddish-violet
                      Mast cell granulesreddish-violet
                      Cartilage matrixreddish-violet
                      Acidophilic materialsorange-red
                      Helicobacter pylori
                      blue to dark blue
                      blue to dark blue
                      Global Trade Item Number
                      Catalog Number GTIN
                      1092040100 04022536111841
                      1092040500 04022536111889
                      1092041000 04022536720081
                      1092042500 04022536111926
                      1092049025 04022536111940

                      Documentation

                      Giemsa's azur eosin methylene blue solution SDS

                      Title

                      Safety Data Sheet (SDS) 

                      Giemsa's azur eosin methylene blue solution Certificates of Analysis

                      Product Number PackagingSpecificationLot Number
                      1092040100 Glass bottlePDF Specification Document
                      1092040500 Plastic bottlePDF Specification Document
                      1092041000 Plastic bottlePDF Specification Document
                      1092042500 Plastic bottlePDF Specification Document
                      1092049025 Barrel PE/metPDF Specification Document

                      Related Products & Applications

                      Related Products

                      Catalog Number Description  
                      109203 Giemsa's azur-eosin-methylene blue Show Pricing & Availability

                      Accessories (Primary)

                      Catalog Number Description  
                      101424 May-Grünwald's eosine-methylene blue solution modified Show Pricing & Availability
                      101728 OSTEOSOFT® Show Pricing & Availability
                      101736 OSTEOMOLL® Show Pricing & Availability
                      106009 Methanol Show Pricing & Availability
                      109016 Neo-Mount™ Show Pricing & Availability
                      109468 Buffer tablets pH 7.2 Show Pricing & Availability
                      109634 2-Propanol Show Pricing & Availability
                      109843 Neo-Clear™ Show Pricing & Availability
                      111374 Buffer tablets pH 6.8 Show Pricing & Availability

                      Accessories (Secondary)

                      Catalog Number Description  
                      101424 May-Grünwald's eosine-methylene blue solution modified Show Pricing & Availability

                      Related Products By: Application Facete

                      Categories

                      IVD/OEM Materials & Reagents > Microscopy > Hematology > Rapid Stainings, Hemacolor®
                      IVD/OEM Materials & Reagents > Microscopy > Histology > Histological Staining Solutions
                      IVD/OEM Materials & Reagents > Microscopy > Cytology > Cytological Staining Reagents
                      IVD/OEM Materials & Reagents > Microscopy > Hematology > Hematological Staining Solutions
                      IVD/OEM Materials & Reagents > Microscopy > Bacteriology > General Bacteriological Staining Solutions