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109217 Gram's safranine solution

for the Gram staining method

Gram's safranine solution MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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109217
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Overview

Replacement Information

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      		 Plastic bottle 500 ml
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      1092172500
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          		 Plastic bottle 2.5 l
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          Description
          Catalogue Number109217
          DescriptionGram's safranine solution
          OverviewThis ready-to-use Gram's safranine solution-for the Gram staining method, is used in bacteriology. The Gram staining allows a fast differentiation of bacteria in Gram-positive and Gram-negative. The mureine structure of the bacteria walls is the basis of the color affinity. Bacteria will be stained with Gram's crystal violet solution (Cat. no.109218) - an aniline dye - in the first step. After the treatment with iodinesolution (Lugol’s solution stabilized with PVP, Cat no.100567), a dye-iodine complex will form. During the decolorizing step (Gram’s decolorizing solution, Cat. no. 110218), this complex stays in the multilayer mureine structures of the Gram-positive bacteria and they will appear blue-violet. Gram-negative bacteria have a monolayer mureine structure only, the dye-iod complex does not stay bound to the cellwall, they will be decolorized . Gram-negative bacteria will be counterstained by Gram's safranin solution and appear pink to red.
          A bottle with 500 ml Gram's safranin solution is sufficient for staining up to 250 slides, it is also available as part of the Gram-color stain set, (Cat. no.1.11885). It is an IVD product and CE registered. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.
          References
          Product Information
          HS Code3212 90 90
          Quality LevelMQ400
          Applications
          Applicationfor the Gram staining method
          Biological Information
          Physicochemical Information
          Density0.98 g/cm3 (20 °C)
          Flash point49 °C
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Hazard Pictogram(s) Hazard Pictogram(s)
          Hazard Statement(s)H226: Flammable liquid and vapour.
          Precautionary Statement(s)P210: Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
          P233: Keep container tightly closed.
          P240: Ground and bond container and receiving equipment.
          P241: Use explosion-proof electrical/ ventilating/ lighting equipment.
          P242: Use non-sparking tools.
          P243: Take action to prevent static discharges.
          Signal WordWarning
          Storage class3 Flammable liquids
          WGKWGK 1 slightly hazardous to water
          Disposal28
          Aqueous solutions: Container D.
          Safety Information
          Categories of dangerflammable
          Product Usage Statements
          Storage and Shipping Information
          StorageStore at +15°C to +25°C.
          Packaging Information
          Transport Information
          Declaration (railroad and road) ADR, RIDKein Gefahrgut
          Declaration (transport by air) IATA-DGRNo Dangerous Good
          Declaration (transport by sea) IMDG-CodeNo Dangerous Good
          Supplemental Information
          Specifications
          Suitability for microscopypasses test
          gram-positve microorganismsblue-violet
          gram-negative microorganismspink to red
          Global Trade Item Number
          Catalog Number GTIN
          1092170500 04022536112046
          1092172500 04022536557397

          Related Products & Applications

          Accessories (Primary)

          Catalog Number Description  
          100567 Lugol's solution Show Pricing & Availability
          109261 Lugol's solution (diluted iodine-potassium iodide solution) Show Pricing & Availability
          110218 Gram's decolorizing solution Show Pricing & Availability

          Accessories (Secondary)

          Catalog Number Description  
          100567 Lugol's solution Show Pricing & Availability
          109261 Lugol's solution (diluted iodine-potassium iodide solution) Show Pricing & Availability

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          Categories

          IVD/OEM Materials & Reagents > Microscopy > Bacteriology > Gram-Bacteria Staining