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SCM303 Hindgut Induction Medium

Ready-to-use serum-free media used for the efficient differentiation of iPSC derived definitive endoderm (DE) cells to CDX-2+ hindgut endoderm cells.

SCM303
50 mL  
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      Overview

      Replacement Information
      Description
      Catalogue NumberSCM303
      DescriptionHindgut Induction Medium
      Alternate Names
      • Hindgut Endoderm Differentiation Medium
      Background InformationThe hindgut is the region of the gut tube lying at the distal third of the transverse colon and gives rise to splenic flexure, descending colon, sigmoid colon and rectum. Additionally, the endodermal layer of the hindgut region forms the epithelial lining of the urinary bladder and urethra. The main function of the hindgut is to absorb most of the water and nutrients remaining from partially digested food.

      The Hindgut Induction Medium is a defined ready-to-use serum-free media used for the efficient differentiation of definitive endoderm cells to CDX-2 positive hindgut endoderm cells. The 4-day differentiation protocol is simple and has been validated on two human iPS cell lines. Differentiated cells express high levels of the hindgut endoderm marker, CDX-2. CDX-2 hindgut endoderm cells can be further differentiated into specialized lineages such as intestinal colon organoids after embedding in Matrigel and cultured in 3dGRO™ Human Colon Organoid Expansion Medium (SCM304).
      References
      Product Information
      Applications
      ApplicationReady-to-use serum-free media used for the efficient differentiation of iPSC derived definitive endoderm (DE) cells to CDX-2+ hindgut endoderm cells.
      Key Applications
      • Stem Cell Culture
      Biological Information
      Media FormLiquid
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assurance• Appearance: Clear liquid/no particulates
      • Sterility Tested: No growth
      • Endotoxin: <2 EU/mL
      • pH: 7.0-7.4
      • Mycoplasma: Negative
      • Functional Assay: >60% CDX-2+ expression after 4 days of differentiation from iPSC derived DE cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsUpon receipt, store at -20ºC. When ready to use, thaw overnight at 2-8°C. Once thawed, mix thoroughly and aliquot into smaller volumes (i.e. 10 mL), use immediately and store at 2-8°C for up to 1 week. Do not re-freeze. Unused aliquots may be stored at -20°C until the expiry date.
      Packaging Information
      Material Size50 mL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      SCM303 04061841036302

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