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EZGRT-89BK Human Ghrelin (total) ELISA- Bulk

This Human Ghrelin (total) ELISA is used to measure & quantify Ghrelin (total) levels in Metabolism & Endocrine & Neuroscience research.

Human Ghrelin (total) ELISA- Bulk MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: ELISA Detection Methods: Fluorescent, Colorimetric Research Category: Metabolism Research Sub-Category: Obesity, Metabolic Disorders
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EZGRT-89BK
Ten 96-well strip plates  This product consists of ten Human Ghrelin (total) ELISA kits.
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      Overview

      Replacement Information

      Key Specifications Table

      Analytes AvailableSpecies ReactivityKey ApplicationsDetection Methods
      Ghrelin (Total) HELISAFluorescent Colorimetric
      Description
      Catalogue NumberEZGRT-89BK
      DescriptionHuman Ghrelin (total) ELISA- Bulk
      Background InformationCirculating ghrelin is a multifunctional hormone secreted primarily by the stomach.
      References
      Product Information
      Detection methodFluorescent Colorimetric
      Precision, %
      • Inter-assay: Serum: 6.2–7.8% Plasma: 5.2–7.7%
      • Intra-assay: Serum: 0.9–1.3% Plasma: 1.1–1.9%
      Quality LevelMQ200
      Applications
      ApplicationThis Human Ghrelin (total) ELISA is used to measure & quantify Ghrelin (total) levels in Metabolism & Endocrine & Neuroscience research.
      Key Applications
      • ELISA
      Application NotesRoom temperature, 3 hour assay, 20 μL sample size (serum or plasma)
      Biological Information
      SpecificityHuman Ghrelin (active) 80% Des-Octanoyl Human Ghrelin 100% Canine Ghrelin (active) 70% Porcine Ghrelin (active) not detectable Motilin Related Peptide (Human, Rat/Mouse) not detectable PYY 3-36 (Human, Mouse, Porcine) not detectable NPY (Human/Rat)
      Species Reactivity
      • Human
      Analytes Available
      • Ghrelin (Total)
      Entrez Gene Number
      Entrez Gene SummaryGhrelin is an endogenous ligand for the growth hormone secretagogue receptor (GHSR; MIM 601898) and is involved in regulating growth hormone (GH; MIM 139250) release. Ghrelin is derived from a preprohormone called preproghrelin, which also generates a second peptide called obestatin. Obestatin is an endogenous ligand for the orphan G protein-coupled receptor GPR39 (MIM 602886) and is involved in satiety and decreased food intake.[supplied by OMIM]
      Gene Symbol
      • GHRL
      • ghrelin
      • obestatin
      • MTLRP
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q9UBU3 # Obestatin may be the ligand for GPR39. May have an appetite-reducing effect resulting in decreased food intake. May reduce gastric emptying activity and jejunal motility (By similarity).
      SIZE: 117 amino acids; 12911 Da
      SUBCELLULAR LOCATION: Secreted.
      TISSUE SPECIFICITY: Highest level in stomach. All forms are found in serum as well. Other tissues compensate for the loss of ghrelin synthesis in the stomach following gastrectomy.
      PTM: O-octanoylation or O-decanoylation is essential for ghrelin activity. The O-decanoylated forms Ghrelin-27-C10 and Ghrelin-28- C10 differ in the length of the carbon backbone of the carboxylic acid bound to Ser-26. A small fraction of ghrelin, ghrelin-28- C10:1, may be modified with a singly unsaturated carboxylic acid. & Amidation of Leu-98 is essential for obestatin activity (By similarity).
      SIMILARITY: SwissProt: Q9UBU3 ## Belongs to the motilin family.
      Accuracy
      • Serum: (Active Ghrelin) 88–91%
      • (Des-octanoyl Ghrelin) 101–107%
      • Plasma: (Active Ghrelin) 84–87%
      • (Des-octanoyl Ghrelin) 112–119%
      Physicochemical Information
      Sensitivity
      • 156 pg/mL (20 µL sample size)
      Linearity of DilutionSerum: 95.7–104.7% Plasma: 95–117%
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Availability by Geography
      • This product is not available for sale in Japan.
      Usage Statement
      • For research use only. Not for use in diagnostic procedures.
      Storage and Shipping Information
      Packaging Information
      Material SizeTen 96-well strip plates
      Material PackageThis product consists of ten Human Ghrelin (total) ELISA kits.
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      EZGRT-89BK 04053252436901

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      Categories

      Life Science Research > Protein Detection and Quantification > Immunoassays > Enzyme-linked Immunosorbent Assay (ELISA)