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IF005 Interferon-γ Protein, Recombinant mouse

The Interferon-gamma protein (or IFN-gamma protein) is a regulatory protein produced by activated NK cells & CD4+TCRalpha/beta+, CD8+TCRalpha/beta+ & TCRgamma/delta+ T cells.

Interferon-γ Protein, Recombinant mouse MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Cell Culture UniProt Number: P01579, P01580
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IF005
100 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Key ApplicationsSpeciesSourcePurity
      CULTMouse Mouse, expressed in E.coliGreater than 98% by SDS-PAGE and HPLC analysis. Endotoxin level is less than 0.1 ng per μg (1EU/μg) of IFN-gamma.
      Description
      Catalogue NumberIF005
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionInterferon-γ Protein, Recombinant mouse
      OverviewInterferon-gamma (IFN-gamma) is a regulatory protein produced by activated NK cells and CD4+TCRalpha/beta+, CD8+TCRalpha/beta+, and TCRgamma/delta+ T cells. The biological activities of IFN-gamma include the specific binding to a single class of high affinity receptors. In addition, IFN-gamma can upgrade the number of lymphoid cell functions including the antimicrobial and antitumor responses of macrophages, NK cells, and neutrophils. Recombinant Murine IFN-γ is a 134 amino-acid length protein with a predicted molecular weight of 15.6 kDa. C-terminal truncation due to proteolytic cleavage occurs during E. coli fermentation and results in a 15.1 kDa protein confirmed by mass spectroscopy. SDS-PAGE analysis of Recombinant Murine IFN-γ shows a band that migrates at approximately 11-12 kDa, under nonreducing and reducing conditions.
      References
      Product Information
      HS Code3502 90 90
      PresentationSterile filtered, lyophilized from 10mM Sodium Phosphate, pH 7.0 + 25mM NaCl.
      Quality LevelMQ100
      Applications
      ApplicationThe Interferon-gamma protein (or IFN-gamma protein) is a regulatory protein produced by activated NK cells & CD4+TCRalpha/beta+, CD8+TCRalpha/beta+ & TCRgamma/delta+ T cells.
      Key Applications
      • Cell Culture
      Application NotesFor most in vitro applications, IFN-gamma exerts its biological activity in the concentration range of 0.1 to 10.0 ng/mL.
      Biological Information
      PurityGreater than 98% by SDS-PAGE and HPLC analysis. Endotoxin level is less than 0.1 ng per μg (1EU/μg) of IFN-gamma.
      SourceMouse, expressed in E.coli
      Specific ActivityThe ED50 as determined by a cytopathic effect inhibition assay with mouse NIH 3T3 cells challenged VSV is <= 0.1 ng/mL, corresponding to a specific activity of >= 1.0 x 10E7 units/mg.
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P01579 # Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.
      SIZE: 166 amino acids; 19348 Da
      SUBUNIT: Homodimer.
      SUBCELLULAR LOCATION: Secreted.
      TISSUE SPECIFICITY: Released primarily from activated T lymphocytes.
      PTM: Proteolytic processing produces C-terminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161.
      DISEASE: SwissProt: P01579 # In Caucasians, genetic variation in IFNG is associated with the risk of aplastic anemia (AA) [MIM:609135]. AA is a rare disease in which the reduction of the circulating blood cells results from damage to the stem cell pool in bone marrow. In most patients, the stem cell lesion is caused by an autoimmune attack. T-lymphocytes, activated by an endogenous or exogenous, and most often unknown antigenic stimulus, secrete cytokines, including IFN-gamma, which would in turn be able to suppress hematopoiesis.
      SIMILARITY: SwissProt: P01579 ## Belongs to the type II (or gamma) interferon family.
      Stem Cell Type
      • Mesenchymal Stem Cells
      • Hematopoietic Stem Cells
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsThe lyophilized protein is stable for a few weeks at room temperature, but is best stored at -20°C. After a quick spin, reconstitute with Sodium Phosphate, pH 8.0 to a concentration of 1 mg/mL. Further dilutions should be made into RPMI + 10% FCS or 5% HAS and stored at 2-8ºC for one week or -20ºC for future use. Reconstituted IFN-gamma should be stored in undiluted aliquots at -20°C for up to six months from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      IF005 04053252335907

      Documentation

      Interferon-γ Protein, Recombinant mouse SDS

      Title

      Safety Data Sheet (SDS) 

      Interferon-γ Protein, Recombinant mouse Certificates of Analysis

      TitleLot Number
      RECOMBINANT MOUSE INTERFERON- - 2090104 2090104
      RECOMBINANT MOUSE INTERFERON- - 2195999 2195999
      RECOMBINANT MOUSE INTERFERON- - 2283317 2283317
      RECOMBINANT MOUSE INTERFERON-#947; - 2003645 2003645
      RECOMBINANT MOUSE INTERFERON-#947; - 2043875 2043875
      RECOMBINANT MOUSE INTERFERON-gamma - LV1573184 LV1573184
      RECOMBINANT MOUSE INTERFERON-γ 2943118
      RECOMBINANT MOUSE INTERFERON-γ 2913905
      RECOMBINANT MOUSE INTERFERON-γ 3030550
      RECOMBINANT MOUSE INTERFERON-γ 3768145
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      Data Sheet

      Title
      RECOMBINANT MOUSE INTERFERON-γ

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