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CCYTOMAG-90K MILLIPLEX Canine Cytokine/Chemokine Magnetic Bead Panel - Immunology Multiplex Assay

Simultaneously analyze multiple cytokine and chemokine biomarkers with Bead-Based Multiplex Assays using the Luminex technology, in canine serum, plasma and cell culture samples.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Multiplexing Detection Methods: Luminex xMAP Research Category: Inflammation & Immunology
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CCYTOMAG-90K

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Overview

Replacement Information
Replacement InformationThis product replaces Canine Cytokine / Chemokine Panel, CCYTO-90K in December of 2011 or while supplies last.

Special Offers

This panel is available for custom premix. Talk to your Protein Specialist today!

Key Specifications Table

Analytes AvailableSpecies ReactivityKey ApplicationsDetection Methods
GM-CSF IFN-γ IL-2 IL-6 IL-7 IL-8 IL-10 IL-15 IL-18 IP-10 KC-like MCP-1 TNF-α CaMplexLuminex xMAP
Description
Catalogue NumberCCYTOMAG-90K
ReplacesCCYTO-90K
Trade Name
  • MILLIPLEX
DescriptionMILLIPLEX Canine Cytokine/Chemokine Magnetic Bead Panel - Immunology Multiplex Assay
OverviewIn addition to studying inflammation for canine nutrition and disease research, the dog is an important animal model for human biomedical research and drug development. Millipore recognizes that the availability of canine-specific immunoassay products is limited. Therefore, the MILLIPLEX Canine Cytokine / Chemokine Panel enables you to focus on the potential role of cytokines in disease, as well as the modulation of cytokine expression. Coupled with the Luminex® xMAP® platform in a magnetic bead format, you receive the advantage of ideal speed and sensitivity, allowing quantitative multiplex detection of dozens of analytes simultaneously, dramatically improving productivity.
Background InformationThe immune system is an organized complex network of biological structures and processes that protect against disease. For example, cytokine and chemokines mediate interactions between cells directly, regulating target immune cell responses. These responses result in inflammation where the immune system attempts to eradicate foreign antigens and begin the healing process. Consequently, the inflammatory process plays a key protective role in immunity. In addition cytokine and chemokine research is essential in understanding the immune system and its multi-faceted response to most antigens, as well as disease states such as autoimmune disease, allergic reactions, sepsis and cancer.
References
Product Information
Detection methodLuminex xMAP
ConfigurationDesign your multiplex kit by choosing available analytes within this panel.
Precision, %
  • Intra-assay: 2 - 4
  • Inter-assay: 8 - 17
Panel TypeMAGNETIC Cytokines/Chemokines
Quality LevelMQ200
Applications
ApplicationSimultaneously analyze multiple cytokine and chemokine biomarkers with Bead-Based Multiplex Assays using the Luminex technology, in canine serum, plasma and cell culture samples.
Key Applications
  • Multiplexing
Application Notes• This is an overnight incubation assay.
• This assay requires 25 µL of a 1:3 dilution of plasma or serum, or 25 μL cell culture supernatant per well.
Biological Information
SpecificityThere was no or negligible cross-reactivity among different analytes within the panel.
Species Reactivity
  • Canine
Analytes Available
  • GM-CSF
  • IFN-γ
  • IL-2
  • IL-6
  • IL-7
  • IL-8
  • IL-10
  • IL-15
  • IL-18
  • IP-10
  • KC-like
  • MCP-1
  • TNF-α
Assay PanelEverything you need in a single kit.
Cross ReactivityCross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
Accuracy
  • 91% - 109%
Physicochemical Information
Sensitivity
  • GM-CSF: 9.2 pg/mL
  • IFN-γ: 13.6 pg/mL
  • IP-10: 3.2 pg/mL
  • IL-2: 3.5 pg/mL
  • IL-6: 3.7 pg/mL
  • IL-7: 7.5 pg/mL
  • IL-8: 21.7 pg/mL
  • IL-10: 8.5 pg/mL
  • IL-15: 9.0 pg/mL
  • IL-18: 5.8 pg/mL
  • KC-like: 5.3 pg/mL
  • MCP-1: 21.0 pg/mL
  • TNFα: 6.1 pg/mL
Linearity of DilutionContact Technical Support for this information.
Standard Curve Range
  • All analytes 12.2 - 50,000 pg/mL
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage for kit components is 2 - 8°C
Packaging Information
Material Size96-well plate
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
CCYTOMAG-90K 04053252988325

Documentation

MILLIPLEX Canine Cytokine/Chemokine Magnetic Bead Panel - Immunology Multiplex Assay SDS

Title

Safety Data Sheet (SDS) 

Technical Info

Title
96 well Template 2 Up
96 well Template Single
CCYTOMAG-90K xPonent Protocol Template Version 3.1
CCYTOMAG-90K xPonent Protocol Template Version Magpix
Quality Control Range Sheet- CYTO-117 and CYTO-217
Quality Control Ranges: Milliplex Canine Cytokine Magnetic Bead Panel

Newsletters / Publications

Title
Article: Evaluation of immunological parameters in pit bull terrier-type dogs with juvenile onset generalized demodicosis and age-matched healthy pit bull terrier-type dogs