Millipore Sigma Vibrant Logo

HBNMAG-51K MILLIPLEX MAP Human Bone Magnetic Bead Panel - Bone Metabolism Multiplex Assay

The analytes available for this multiplex kit are: ACTH, DKK-1, FGF-23, IL-1β, IL-6, Insulin, Leptin, Osteocalcin, OPN - Osteopontin, Osteoprotegerin, PTH, SOST, TNF-α.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Multiplexing Detection Methods: Luminex xMAP Research Category: Metabolism
View Products on Sigmaaldrich.com
HBNMAG-51K
Design And Price Your Kit

Overview

Replacement Information

Key Specifications Table

Analytes AvailableSpecies ReactivityKey ApplicationsDetection Methods
ACTH DKK-1 FGF-23 IL-1β IL-6 Insulin Leptin Osteocalcin OPN - Osteopontin Osteoprotegerin PTH SOST TNF-α HMplexLuminex xMAP
Description
Catalogue NumberHBNMAG-51K
ReplacesHBN1B-51K
Trade Name
  • MILLIPLEX
DescriptionMILLIPLEX MAP Human Bone Magnetic Bead Panel - Bone Metabolism Multiplex Assay
Background InformationThe Milliplex® MAP Human Bone Magnetic Bead Panel contains all the components necessary to measure 13 biomarkers in any combination: ACTH (Adrenocorticotropic hormone), DKK-1 (Dickkopf-1), IL-6, Insulin, Leptin, TNFα, OPG (Osteopotegrin), OC (Osteocalcin), OPN (Osteopontin), SOST (Sclerostin), IL-1β, PTH (parathyroid) and FGF-23 in any combination. The kit uses a 96-well format, contains a lyophilized standard cocktail, 2 quality controls and can measure up to 38 serum or plasma samples in duplicate.
References
Product Information
Detection methodLuminex xMAP
ConfigurationDesign your multiplex kit by choosing available analytes within this panel.
Precision, %
  • Inter-assay: < 15
  • Intra-Assay: < 10
Panel TypeMAGNETIC Bone
Quality LevelMQ200
Applications
ApplicationThe analytes available for this multiplex kit are: ACTH, DKK-1, FGF-23, IL-1β, IL-6, Insulin, Leptin, Osteocalcin, OPN - Osteopontin, Osteoprotegerin, PTH, SOST, TNF-α.
Key Applications
  • Multiplexing
Application NotesThis assay is a same day assay (2 hour incubation) or overnight assay (16 hour incubation). Serum and plasma samples require (recommend) a 1:2 dilution with Assay Buffer. Each well requires 25 μL of sample, standard or control. Tissue culture supernatants can also be used and if needed, make appropriate dilutions with control medium.
Biological Information
Species Reactivity
  • Human
Analytes Available
  • ACTH
  • DKK-1
  • FGF-23
  • IL-1β
  • IL-6
  • Insulin
  • Leptin
  • Osteocalcin
  • OPN - Osteopontin
  • Osteoprotegerin
  • PTH
  • SOST
  • TNF-α
Assay PanelEverything you need in a single kit.
Cross ReactivityNo cross reactivity between the antibodies for an analyte in this panel were observed.
Accuracy
  • 82% - 97%
Physicochemical Information
Sensitivity
  • See kit protocol for individual analytes sensitivities.
Linearity of DilutionContact technical support for this information
Standard Curve Range
  • ACTH & IL-6: 1-6,000 pg/mL
  • DKK-1: 5-20,000 pg/mL
  • Insulin: 61-250,000 pg/mL
  • Leptin: 49-200,000 pg/mL
  • TNFα: 0.24-1,000 pg/mL
  • OPG: 7-30,000 pg/mL
  • OC: 146-600,000 pg/mL
  • OPN: 98-400,000 pg/mL
  • SOST: 24-100,000 pg/mL
  • IL-1β: 1-3,000 pg/mL
  • PTH: 5-20,000 pg/mL
  • FGF-23: 37-150,000 pg/mL
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage for kit components is 2 - 8°C.
Packaging Information
Material Size96-well plate
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
HBNMAG-51K 04053252988417