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HPLX1-115SP MILLIPLEX® PLEXpedition Magnetic Bead Screening Panel

•GIP and ENA-78 have low cross-reactivity with IL-23 (≤15%) and GRO (≤5%) recombinant proteins, respectively.
•IL-12 (p40) has moderate, but expected, cross-reactivity with the recombinant proteins for IL-12 (p70) and IL-23 which both contain the p40 subunit.
•MMP-3 may exhibit significant cross-reactivity with MMP-10 recombinant proteins due to high sequence homology, however, analysis demonstrated independent and differentiated measurement of MMP-3 and MMP-10 in biological samples.

HPLX1-115SP
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Overview

Replacement Information
Description
Catalogue NumberHPLX1-115SP
Trade Name
  • MILLIPLEX
DescriptionMILLIPLEX® PLEXpedition Magnetic Bead Screening Panel
OverviewThe MILLIPLEX® PLEXpedition Screening Panel is a configurable multiplex immunoassay kit developed to simultaneously screen up to 115 targets including cytokines, chemokines, growth factors, matrix metalloproteinases (MMPs), and biomarkers of bone health, metabolism, and cardiovascular disease. Two Luminex® instruments, the xMAP® INTELLIFLEX and the FLEXMAP 3D®, can analyze all 115 bead regions with comparable sensitivity and sample correlation while the MAGPIX® and Luminex® 200™ instruments can detect specific subsets of 50 and 80 analytes, respectively. As a discovery biomarker tool this panel is provided in a matrix-free format to enable analyte detection in multiple sample types. After screening with PLEXpedition to identify markers of interest, customers can transition to our Qualified MILLIPLEX® panels, each of which has an optimized serum matrix and lot-to-lot verification for reproducible quantification of protein concentration.

MILLIPLEX® Screening Assays
• Contain all reagents necessary to conveniently run the assay including premixed beads and premixed detection antibodies
• Verify analyte specificity across the entire panel
• Are sample agnostic for greater flexibility
• Enable easy transition to Qualified Assays by utilizing same critical raw materials
Background InformationThe MILLIPLEX® PLEXpedition Magnetic Bead Screening Panel is to be used for the simultaneous quantification of up to 115 analytes in your selected sample type. (NOTE: Instrument requirements vary based on analyte selection.)

• MAGPIX®/Luminex® 200™/FLEXMAP 3D®/xMAP® INTELLIFLEX required for any or all of the following 50 analytes: EGF, FGF-2, Fractalkine, G-CSF, Ghrelin (Active), GIP, Glucagon, GM-CSF, Granzyme B, GROα, HGF, IFNα2, IFNγ, IL-10, IL-12 (p40), IL-12 (p70), IL-13, IL-15, IL-17A, IL-18, IL-1Ra, IL-1α, IL-1β, IL-2, IL-22, IL-23, IL-4, IL-5, IL-6, IL-7, IL-8, IP-10, Leptin, MCP-1, MIG, MIP-1α, MIP-1β, OPN, PDGF-AA, PDGF-BB, Perforin, RANTES, sCD40L, SCF, sFAS, sFASL, TGFα, TNFα, TRAIL, VEGF-A

• Luminex® 200™/FLEXMAP 3D®/xMAP® INTELLIFLEX required for any or all of the following 80 analytes: ACTH, BDNF, C-Peptide, EGF, Eotaxin, FABP4, FGF-2, FLT3 Ligand, Fractalkine, G-CSF, Ghrelin (Active), GIP, GLP-1 Total, Glucagon, GM-CSF, Granzyme A, Granzyme B, GROα, HGF, IFNα2, IFNγ, IL-10, IL-12 (p40), IL-12 (p70), IL-13, IL-15, IL-17A, IL-17F, IL-18, IL-1Ra, IL-1α, IL-1β, IL-2, IL-21, IL-22, IL-23, IL-3, IL-33, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, Insulin, IP-10, I-TAC, Leptin, MCP-1, MCP-3, M-CSF, MDC, MIG, MIP-1α, MIP-1β, MIP-3α, MMP-1, MMP-2, MMP-7, MMP-9, OPG, OPN, PDGF-AA, PDGF-BB, Perforin, PlGF, PP, PYY, RANTES, sCD40L, SCF, sFAS, sFASL, TARC, TGFα, TNFα, TNFβ, TRAIL, Troponin I, VEGF-A

• FLEXMAP 3D®/xMAP® INTELLIFLEX required for any or all of the following 115 analytes: ACTH, Amylin (Active), BCA-1, BDNF, BNP, CK-MB, C-Peptide, DKK1, EGF, ENA-78, Eotaxin, Eotaxin-2, Eotaxin-3, Erythropoeitin (EPO), FABP3, FABP4, FGF-2, FGF-21, FGF-23, FLT3 Ligand, Fractalkine, G-CSF, Ghrelin (Active), GIP, GLP-1 Total, Glucagon, GM-CSF, GPC-2, Granzyme A, Granzyme B, GROα, HB-EGF, HGF, I-309, IFNα2, IFNγ, IL-10, IL-11, IL-12 (p40), IL-12 (p70), IL-13, IL-15, IL-17A, IL-17F, IL-18, IL-1Ra, IL-1α, IL-1β, IL-2, IL-21, IL-22, IL-23, IL-3, IL-33, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, Insulin, IP-10, I-TAC, Leptin, LIF, MCP-1, MCP-2, MCP-3, MCP-4, M-CSF, MDC, MIG, MIP-1α, MIP-1β, MIP-1Ϭ, MIP-3α, MIP-3β, MMP-1, MMP-10, MMP-12, MMP-2, MMP-3, MMP-7, MMP-9, Myostatin , NGF, NTproBNP, OPG, OPN, Osteocalcin, Osteonectin, PDGF-AA, PDGF-BB, Perforin, PlGF, PP, PYY, RANTES, sCD40L, SCF, SDF-1, sFAS, sFASL, SOST, TARC, TGFα, TNFα, TNFβ, TPO, TRAIL, Troponin I, TSLP, VEGF-A, VEGF-C, VEGF-D
References
Product Information
Detection methodLuminex xMAP
ConfigurationDesign your multiplex kit by choosing available analytes within this panel.
Precision, %
  • Intra-assay: <15%
  • Inter-assay: <20%
  • (<25% for PDGF-AA)
Panel TypeMAGNETIC Immune Response
Quality LevelMQ100
Applications
Application•GIP and ENA-78 have low cross-reactivity with IL-23 (≤15%) and GRO (≤5%) recombinant proteins, respectively.
•IL-12 (p40) has moderate, but expected, cross-reactivity with the recombinant proteins for IL-12 (p70) and IL-23 which both contain the p40 subunit.
•MMP-3 may exhibit significant cross-reactivity with MMP-10 recombinant proteins due to high sequence homology, however, analysis demonstrated independent and differentiated measurement of MMP-3 and MMP-10 in biological samples.
Key Applications
  • Multiplexing
Application Notes• This is an overnight assay.
• Each assay requires 25 µL per well of neat or diluted sample.
• Each kit includes premixed beads and premixed detection antibodies.
Biological Information
Species Reactivity
  • Human
Analytes Available
  • ACTH
  • Amylin (Active)
  • BCA-1
  • BDNF
  • BNP
  • CK-MB
  • C-Peptide
  • DKK-1
  • EGF
  • ENA-78
  • Eotaxin
  • Eotaxin-2
  • Eotaxin-3
  • Erythropoietin (EPO)
  • FABP3
  • FABP4
  • FGF-2
  • FGF-21
  • FGF-23
  • Flt-3 ligand
  • Fractalkine
  • G-CSF
  • Ghrelin (Active)
  • GIP
  • GLP-1 (Total)
  • Glucagon
  • GM-CSF
  • Granzyme A
  • Granzyme B
  • GROα
  • HB-EGF
  • HGF
  • I-309
  • IFNα2
  • IFNγ
  • IL-10
  • IL-11
  • IL-12 (p40)
  • IL-13
  • IL-15
  • IL-17A
  • IL-17F
  • IL-18
  • IL-1Rα
  • IL-1α
  • IL-1β
  • IL-2
  • IL-21
  • IL-22
  • IL-23
  • IL-3
  • IL-33
  • IL-4
  • IL-5
  • IL-6
  • IL-7
  • IL-8
  • IL-9
  • Insulin
  • IP-10
  • I-TAC
  • Leptin
  • LIF
  • MCP-1
  • MCP-2
  • MCP-3
  • MCP-4
  • M-CSF
  • MDC (CCL22)
  • MIG
  • MIP-1α
  • MIP-1β
  • MIP-1d
  • MIP-3α
  • MIP-3β
  • MMP-1
  • MMP-10
  • MMP-12
  • MMP-2
  • MMP-3
  • MMP-7
  • MMP-9
  • Myostatin/GDF8
  • NGF
  • NT-proBNP
  • OPG
  • OPN - Osteopontin
  • Osteocalcin
  • Osteonectin (SPARC)
  • PDGF-AA
  • PDGF-BB
  • Perforin
  • PIGF-2
  • PP
  • PYY
  • RANTES
  • sCD40L
  • SCF
  • SDF-1
  • sFas
  • sFasL
  • SOST
  • TARC
  • TGFα
  • TNFα
  • TNFβ
  • TPO
  • TRAIL
  • Troponin I (TnI)
  • TSLP
  • VEGF-A
  • VEGF-C
  • VEGF-D
  • GCP2
  • IL-12 (p70)
Cross ReactivityThere was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes in this panel, with the following exceptions: (See Applications)
Physicochemical Information
Sensitivity
  • Refer to kit protocol for sensitivities for individual analytes.
Standard Curve Range
  • Refer to lot specific product inserts for analyte concentrations.
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage for kit components is 2 - 8°C.
Packaging Information
Material Size96-well plate
Material Package96 well plate
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
HPLX1-115SP 04065270783196

Documentation

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