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14-377 MKK4/SKK1 Protein, active, 10 µg

Active, N-terminal GST tagged, murine MKK4 residues 34-end, for use in Kinase Assays.

MKK4/SKK1 Protein, active, 10 µg MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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14-377
10 µg  Also available in 250 µg size --call for pricing and availability and reference catalog number 14-377M when ordering the 250 µg size.
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      Overview

      Replacement Information
      Description
      Catalogue Number14-377
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionMKK4/SKK1 Protein, active, 10 µg
      OverviewN-terminal GST tagged, murine MKK4 residues 34-end
      References
      Product Information
      Quality LevelMQ100
      Applications
      ApplicationActive, N-terminal GST tagged, murine MKK4 residues 34-end, for use in Kinase Assays.
      Key Applications
      • Kinase Assay
      Biological Information
      Sourceexpressed in E. coli
      Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a dual specificity protein kinase that belongs to the Ser/Thr protein kinase family. This kinase is a direct activator of MAP kinases in response to various environmental stresses or mitogenic stimuli. It has been shown to activate MAPK8/JNK1, MAPK9/JNK2, and MAPK14/p38, but not MAPK1/ERK2 or MAPK3/ERK3. This kinase is phosphorylated, and thus activated by MAP3K1/MEKK. The knockout studies in mice suggested the roles of this kinase in mediating survival signal in T cell development, as well as in the organogenesis of liver.
      Gene Symbol
      • MAP2K4
      • MKK4
      • JNKK
      • MEK4
      • MAPKK4
      • JNKK1
      • SEK1
      • SERK1
      • PRKMK4
      Protein TargetMKK4
      Purification MethodPurified using glutathione agarose. Activated by incubation with MEKK and then re-purified using Ni2+/NTA agarose
      Target Sub-FamilySTE
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P45985 # Dual specificity kinase that activates the JUN kinases MAPK8 (JNK1) and MAPK9 (JNK2) as well as MAPK14 (p38) but not MAPK1 (ERK2) or MAPK3 (ERK1).
      SIZE: 399 amino acids; 44288 Da
      SUBUNIT: Interacts with SPAG9 (By similarity).
      TISSUE SPECIFICITY: Abundant expression is seen in the skeletal muscle. It is also widely expressed in other tissues.
      PTM: Activated by phosphorylation on Ser/Thr by MAP kinase kinase kinases (By similarity).
      SIMILARITY: SwissProt: P45985 ## Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily. & Contains 1 protein kinase domain.
      Molecular Weight67.7kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by phosphorylation of ATF2 substrate
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions6 months at -70C
      Packaging Information
      Material Size10 µg
      Material PackageAlso available in 250 µg size --call for pricing and availability and reference catalog number 14-377M when ordering the 250 µg size.
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      14-377 04053252673337

      Documentation

      MKK4/SKK1 Protein, active, 10 µg SDS

      Title

      Safety Data Sheet (SDS) 

      MKK4/SKK1 Protein, active, 10 µg Certificates of Analysis

      TitleLot Number
      MKK4/SKK1, active - D8JN050U-M D8JN050U-M
      MKK4/SKK1, active - D8JN050U-N D8JN050U-N
      MKK4/SKK1, active - D8JN050U-O D8JN050U-O
      MKK4/SKK1, active - D8JN050U-P D8JN050U-P
      MKK4/SKK1, active - D8JN050U-Q D8JN050U-Q

      References

      Reference overviewApplicationPub Med ID
      Synergistic activation of stress-activated protein kinase 1/c-Jun N-terminal kinase (SAPK1/JNK) isoforms by mitogen-activated protein kinase kinase 4 (MKK4) and MKK7
      Fleming, Y., et al
      Biochem J, 352 Pt 1:145-54 (2000)  2000

      		Kinase Assay11062067 11062067

      Related Products & Applications

      Alternative Packsize

      Catalog Number Description  
      14-377M MKK4/SKK1 Protein, active, 250 µg Show Pricing & Availability

      Product Families

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      Categories

      Life Science Research > Proteins and Enzymes > Purified Kinases
      Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Kinases