Our broad portfolio consists of multiplex panels that allow you to choose, within the panel, analytes that best meet your needs. On a separate tab you can choose the premixed cytokine format or a single plex kit.
Cell Signaling Kits & MAPmates™
Choose fixed kits that allow you to explore entire pathways or processes. Or design your own kits by choosing single plex MAPmates™, following the provided guidelines.
The following MAPmates™ should not be plexed together:
-MAPmates™ that require a different assay buffer
-Phospho-specific and total MAPmate™ pairs, e.g. total GSK3β and GSK3β (Ser 9)
-PanTyr and site-specific MAPmates™, e.g. Phospho-EGF Receptor and phospho-STAT1 (Tyr701)
-More than 1 phospho-MAPmate™ for a single target (Akt, STAT3)
-GAPDH and β-Tubulin cannot be plexed with kits or MAPmates™ containing panTyr
.
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Select A Species, Panel Type, Kit or Sample Type
To begin designing your MILLIPLEX® MAP kit select a species, a panel type or kit of interest.
Custom Premix Selecting "Custom Premix" option means that all of the beads you have chosen will be premixed in manufacturing before the kit is sent to you.
If you have chosen panel analytes and then choose a premix or single plex kit, you will lose that customization.
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96-Well Plate
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Add Additional Reagents (Buffer and Detection Kit is required for use with MAPmates)
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48-602MAG
Buffer Detection Kit for Magnetic Beads
1 Kit
Space Saver Option Customers purchasing multiple kits may choose to save storage space by eliminating the kit packaging and receiving their multiplex assay components in plastic bags for more compact storage.
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Membrane-spanning 4-domains subfamily A member 1 B-lymphocyte surface antigen B1 Leukocyte surface antigen Leu-16 Bp35 CD_antigen=CD20
Background Information
CD20 is a transmembrane, non-glycosylated protein expressed on B-cell precursors and mature B cells, but is lost following differentiation into plasma cells (Tedder, 1994). In resting B cells, CD20 appears in a 33 kDa non-phosphorylated form. After mitogen stimulation, CD20 becomes heavily phosphorylated (35-37 kDa isoforms), and it is a dominant phosphoprotein in activated B cells and B-cell lines (Zhou, 1995). The long N- and C-terminal ends of the protein are located on the cytoplasmic side of the membrane and only a minor portion of the protein is exposed on the cell surface (Tedder, 1994). Antibodies reacting with CD20 cytoplasmic epitopes are designated CD20cy (Zhou, 1995). It is suggested that CD20 plays a direct role in regulating the transmembrane conductive Ca 2+ flux of B cells which indicates a possible function for CD20 as a regulator of proliferation and differentiation (Tedder, 1994).
References
Product Information
Format
FITC
Control
Human Lymphocytes
Presentation
Purified Mouse monoclonal IgG1k conjugated to PE in 0.05M Tris-HCl buffer with 1% BSA, Sodium Azide at 0.097% at pH 7.2
Evaluated by flow cytometry using human lymphocytes.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Maintain refrigerated at 2-8 °C protected from light in undiluted aliquots for up to 6 months from date of receipt.
MilliporeSigma's Milli-Mark and FlowCellect brands include fluorescent conjugated antibodies and multiparameter kits for cellular analysis. Designed, optimized, and validated for flow. Learn More >>
