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70666 Ni-NTA His•Bind® Resin

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70666
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70666-3
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      Plastic ampoule 10 ml
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      70666-4
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          Glass bottle 25 ml
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          70666-5
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              Glass bottle 100 ml
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              70666-6
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                  Glass bottle 500 ml
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                  Description
                  OverviewNi-NTA His•Bind® Resin is a high-performance Ni2+-charged agarose used for rapid one-step purification of proteins containing a His•Tag® sequence by metal chelation chromatography. NTA chemistry minimizes metal leaching during purification and is compatible with up to 10 mM β-mercaptoethanol or 1 mM Tris(hydroxypropyl)phosphine (THP) for reduction of disulfide bonds. Ni-NTA His•Bind® Resin has a binding capacity of 5–10 mg His•Tag® fusion protein per ml resin.

                  Supplied as a 50% slurry; the quantity/pack is based on the amount of the settled resin.
                  Catalogue Number70666
                  Brand Family Novagen®
                  References
                  Product Information
                  Quality LevelMQ400
                  Applications
                  Biological Information
                  Physicochemical Information
                  Dimensions
                  Materials Information
                  Toxicological Information
                  Safety Information according to GHS
                  Safety Information
                  R PhraseR: 10-22-42/43

                  Flammable.
                  Harmful if swallowed.
                  May cause sensitization by inhalation and skin contact.
                  S PhraseS: 26-36-7

                  In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
                  Wear suitable protective clothing.
                  Keep container tightly closed.
                  Product Usage Statements
                  Storage and Shipping Information
                  Ship Code Shipped at Ambient Temperature or with Blue Ice
                  Toxicity Flammable
                  Hazardous Materials Attention: Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
                  Storage +2°C to +8°C
                  Do not freeze Yes
                  Packaging Information
                  Transport Information
                  Supplemental Information
                  Specifications
                  Global Trade Item Number
                  Catalog Number GTIN
                  70666-3 07790788054946
                  70666-4 07790788052768
                  70666-5 04055977334074
                  70666-6 04055977256826

                  Documentation

                  Ni-NTA His•Bind® Resin SDS

                  Title

                  Safety Data Sheet (SDS) 

                  Ni-NTA His•Bind® Resin Certificates of Analysis

                  TitleLot Number
                  70666

                  Technical Info

                  Title
                  Ni-NTA HisユBind® Resins

                  Citations

                  Title
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                • Ganna Panasyuk, et al. (2006) Nuclear export of S6K1 II is regulated by protein kinase CK2 phosphorylation at ser 17. Journal of Biological Chemistry 281, 31188-31201.
                • Lauren E. Ball, Mary N. Berkaw and Maria G. Buse. (2005) Identification of the major site of O-linked ß-N-acetylglucosamine modification in the C-terminus of insulin receptor substrate-1. Molecular and Cellular Proteomics 5, 313-323.
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                • Maria V. Lara, et al. (2005) NADP-malic enzyme and Hsp70: co-purification of both proteins and modification of NADP-malic enzyme properties by association with Hsp70. Plant and Cell Physiology 46, 997-1006.
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                • Aileen Aherne, et al. (2004) On the molecular patholo
                • User Protocols

                  Title
                  TB273 Ni-NTA His•Bind® Resin

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