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482702 Nitric Oxide Synthase Assay Kit, Colorimetric

482702
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Overview

Key Specifications Table

Detection Methods
Colorimetric

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482702-1KIT
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      Description
      OverviewAssay kit useful for the rapid and accurate measurement of nitric oxide synthase (NOS) activity. This colorimetric assay uses lactate dehydrogenase (LDH) to destroy excess NADPH (cofactor for NOS) which interferes with the chemistry of Griess Reagent, commonly used for nitrite/nitrate detection. Do not use with nitrate- or nitrite-containing tissue culture media such as RPMI.
      Catalogue Number482702
      Brand Family Calbiochem®
      SynonymsNOS Assay Kit, Colorimetric
      Materials Required but Not Delivered A plate reader with 540 nm filter
      An adjustable pipettor
      Glass distilled water or HPLC-grade water
      References
      ReferencesNims, R.W., et al. 1995. Methods Enzymol. 7, 48.
      Moncada, S. 1992. Acta Physiol. Scand. 145, 201.
      Nathan, C. 1992. FASEB J. 6, 3051.
      Green, L.C., et al. 1982. Anal. Biochem. 126, 131.
      Product Information
      Detection methodColorimetric
      Form96 Tests
      Format96-well plate
      Kit containsAssay Buffer, Nitrate Reductase, Cofactors, Nitrate Standard, Lactate Dehydrogenase, Griess Reagents (R1 and R2), Microtiter Plate, Plate Cover, and a user protocol.
      Quality LevelMQ100
      Biological Information
      Assay time2 h
      Sample TypePlasma, serum, urine, tissue culture medium, and tissue homogenates
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Storage ConditionsUpon arrival store the Microplate at room temperature and all other components of the kit at -20°C.
      Do not freeze Ok to freeze
      Supplemental Information
      Kit containsAssay Buffer, Nitrate Reductase, Cofactors, Nitrate Standard, Lactate Dehydrogenase, Griess Reagents (R1 and R2), Microtiter Plate, Plate Cover, and a user protocol.
      Global Trade Item Number
      Catalog Number GTIN
      482702-1KIT 04055977200614