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14-621M PTP-1B Protein, 250 µg

N-terminal GST-tagged, recombinant, human PTP-1B, amino acids 1-321, for use in Phosphatase Assays.

14-621M
250 µg  Also available in 10 μg size (cat#14-621) and in bulk (cat#14-621-K).
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      Overview

      Replacement Information
      Description
      Catalogue Number14-621M
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionPTP-1B Protein, 250 µg
      OverviewN-terminal GST-tagged, recombinant, human PTP-1B, amino acids 1-321
      References
      Product Information
      Quality LevelMQ300
      Applications
      ApplicationN-terminal GST-tagged, recombinant, human PTP-1B, amino acids 1-321, for use in Phosphatase Assays.
      Key Applications
      • Phosphatase Assay
      Biological Information
      Sourcehuman PTP-1B expressed in E. coli
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is the founding member of the protein tyrosine phosphatase (PTP) family, which was isolated and identified based on its enzymatic activity and amino acid sequence. PTPs catalyze the hydrolysis of the phosphate monoesters specifically on tyrosine residues. Members of the PTP family share a highly conserved catalytic motif, which is essential for the catalytic activity. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP has been shown to act as a negative regulator of insulin signaling by dephosphorylating the phosphotryosine residues of insulin receptor kinase. This PTP was also reported to dephosphorylate epidermal growth factor receptor kinase, as well as JAK2 and TYK2 kinases, which implicated the role of this PTP in cell growth control, and cell response to interferon stimulation.
      Gene Symbol
      • PTPN1
      • PTP1B
      • PTP-1B
      Protein TargetPTP-1B
      Purification MethodGlutathione-agarose
      Target Sub-FamilyTyr phosphatase
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P18031 # May play an important role in CKII- and p60c-src-induced signal transduction cascades (By similarity).
      SIZE: 435 amino acids; 49967 Da
      SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side.
      PTM: Oxidized on Cys-215; the Cys-SOH formed in response to redox signaling reacts with the alpha-amido of the following residue to form a 4-amino-3-isothiazolidinone serine cross-link, triggering a conformational change that inhibits substrate binding and activity. The active site can be restored by reduction.
      SIMILARITY: SwissProt: P18031 ## Belongs to the protein-tyrosine phosphatase family. Non-receptor class 1 subfamily. & Contains 1 tyrosine-protein phosphatase domain.
      Molecular Weight64.1kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by the dephosphorylation of 100μM DiFMUP
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions6 months at -70°C
      Packaging Information
      Material Size250 µg
      Material PackageAlso available in 10 μg size (cat#14-621) and in bulk (cat#14-621-K).
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      14-621M 04055977311259

      Documentation

      Related Products & Applications

      Alternative Packsize

      Catalog Number Description  
      14-621 PTP-1B Protein, 10 µg Show Pricing & Availability

      Product Families

      Kinase & Phosphatase Products

      Millipore offers a large portfolio of Kinases, Phophatases, Substrates, Inhibitors, Kits, reagents & tools for Signaling research. See below for a list of these products, in multiple pack sizes.
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      Categories

      Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Phosphatases
      Life Science Research > Proteins and Enzymes > Purified Phosphatases