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525320 PhosphoDetect™ Anti-Phosphotyrosine Mouse mAb (PY20) Peroxidase Conjugate

This PhosphoDetect™ Anti-Phosphotyrosine Mouse mAb (PY20) Peroxidase Conjugate is validated for use in ELISA, Immunoblotting, Immunohistochemistry for the detection of Phosphotyrosine.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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525320
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Overview

Replacement Information

Key Specifications Table

Species ReactivityHostAntibody Type
A Broad Range Of SpeciesMMonoclonal Antibody

Pricing & Availability

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525320-100UG
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      		 Plastic ampoule 100 μg
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      Description
      OverviewPeroxidase conjugate of Anti-Phosphotyrosine Mouse mAb (PY20), Cat. No. 525295. Recognizes a broad spectrum of tyrosine-phosphorylated proteins in their native and denatured states in crude extracts.
      Catalogue Number525320
      Brand Family Calbiochem®
      References
      ReferencesGlenney, J.R., et al. 1988. J. Immunol. Methods 109, 277.
      Product Information
      FormLiquid
      FormulationIn PBS, 50% glycerol, 1% BSA, pH 7.4.
      Preservative0.1% Proclin
      Quality LevelMQ100
      Applications
      Key Applications Enzyme-Linked Immunosorbent Assay
      Immunoblotting (Western Blotting)
      Immunohistochemistry
      Application NotesELISA (1:2000)
      Immunoblotting (1:500-1:2000)
      Immunohistochemistry (1:500)
      Application CommentsSpecific for tyrosine phosphorylated proteins in both the native and denatured states. Binding is inhibited by phosphotyrosine; does not react with threonine or serine phosphorylated residues on proteins. Do not use milk as a blocking agent with this antibody. Milk contains many phosphotyrosine containing proteins and will adsorb the antibody. Use a buffer with < 150 mM NaCl and divalent cations not much >1 mM because this antibody's binding is inhibited by high salt concentrations and divalent cations. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      ImmunogenKLH conjugated to phosphotyrosine, glycine, and alanine
      ClonePY20
      HostMouse
      IsotypeIgG2b
      Species Reactivity
      • A Broad Range Of Species
      Antibody TypeMonoclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Ok to freeze
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      525320-100UG 07790788051129

      Documentation

      PhosphoDetect™ Anti-Phosphotyrosine Mouse mAb (PY20) Peroxidase Conjugate SDS

      Title

      Safety Data Sheet (SDS) 

      PhosphoDetect™ Anti-Phosphotyrosine Mouse mAb (PY20) Peroxidase Conjugate Certificates of Analysis

      TitleLot Number
      525320

      References

      Reference overview
      Glenney, J.R., et al. 1988. J. Immunol. Methods 109, 277.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision03-December-2007 JSW
      ApplicationELISA (1:2000)
      Immunoblotting (1:500-1:2000)
      Immunohistochemistry (1:500)
      DescriptionPurified mouse monoclonal antibody conjugated to horseradish peroxidase. Recognizes a broad spectrum of tyrosine-phosphorylated proteins.
      BackgroundCellular activation, proliferation and differentiation are signaled by phosphorylation of tyrosine residues. Growth factor receptors and oncogene protein kinases are associated with tyrosine phosphorylation. Anti-phosphotyrosine antibodies are indispensable for the study of cellular tyrosine phosphorylation.
      HostMouse
      ImmunogenKLH conjugated to phosphotyrosine, glycine, and alanine
      ClonePY20
      IsotypeIgG2b
      Speciesa broad range of species
      FormLiquid
      FormulationIn PBS, 50% glycerol, 1% BSA, pH 7.4.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative0.1% Proclin
      CommentsSpecific for tyrosine phosphorylated proteins in both the native and denatured states. Binding is inhibited by phosphotyrosine; does not react with threonine or serine phosphorylated residues on proteins. Do not use milk as a blocking agent with this antibody. Milk contains many phosphotyrosine containing proteins and will adsorb the antibody. Use a buffer with < 150 mM NaCl and divalent cations not much >1 mM because this antibody's binding is inhibited by high salt concentrations and divalent cations. Variables associated with assay conditions will dictate the proper working dilution.
      Storage +2°C to +8°C
      Do Not Freeze Ok to freeze
      Toxicity Standard Handling
      ReferencesGlenney, J.R., et al. 1988. J. Immunol. Methods 109, 277.