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676604
Sigma-AldrichPhosphoDetect™ Anti-VASP (pSer¹⁵⁷) Mouse mAb (5C6)
This PhosphoDetect™ Anti-VASP (pSer¹⁵⁷) Mouse mAb (5C6) is validated for use in ELISA, Immunoblotting, ICC, IP for the detection of VASP (pSer¹⁵⁷).
More>>This PhosphoDetect™ Anti-VASP (pSer¹⁵⁷) Mouse mAb (5C6) is validated for use in ELISA, Immunoblotting, ICC, IP for the detection of VASP (pSer¹⁵⁷). Less<<
MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Detection of human VASP, phosphorylated on Ser157, by immunoblotting. Samples: Whole cell extracts from human platelets cells left untreated (lane 1) or SNP treated cells (lane 2). Primary antibody: PhosphoDetect™ Anti-VASP (pSer157) Mouse mAb (5C6) (Cat. No. 676604) (0.5 µg/ml). Detection: chemiluminescence.
References
References
Bear, J.E., et al. 2001. Curr. Opin. Cell. Biol.13, 158. Lawrence, D.W., and Pryzwansky, K.B. 2001. J. Immunol.166, 5550. Burkhardt, M., et al. 2000. J. Biol. Chem.275, 33536. Harbeck, B., et al. 2000. J. Biol. Chem.275, 40. Smolenski, A., et al. 2000. J. Biol. Chem.275, 25723.
Does not cross-react with non-phosphorylated VASP or with unrelated phosphorylation sites. For immunocytochemistry, fixation protocol may include up to 0.5% formaldehyde. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
Immunogen
a synthetic phosphopeptide corresponding to amino acids surrounding the Ser¹⁵⁷ phosphorylation site of human VASP
Immunogen
Human
Clone
5C6
Host
Mouse
Isotype
IgG₁
Species Reactivity
Human
Mouse
Antibody Type
Monoclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code
Ambient Temperature Only
Toxicity
Standard Handling
Storage
-20°C
Do not freeze
Ok to freeze
Special Instructions
Reconstitute in 1 ml sterile, dH₂O, 15 min, room temperature. Following reconstitution, refrigerate (4°C) for short-term storage or aliquot, flash-freeze in liquid nitrogen, and freeze (-70°C) for long-term storage. Stock solutions are stable for up to 3 months at 4°C or for up to 1 year at -70°C. Thaw aliquots at 37°C. Avoid freeze/thaw cycles of solutions.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number
GTIN
676604-100UG
04055977260564
Documentation
PhosphoDetect™ Anti-VASP (pSer¹⁵⁷) Mouse mAb (5C6) SDS
PhosphoDetect™ Anti-VASP (pSer¹⁵⁷) Mouse mAb (5C6) Certificates of Analysis
Title
Lot Number
676604
References
Reference overview
Bear, J.E., et al. 2001. Curr. Opin. Cell. Biol.13, 158. Lawrence, D.W., and Pryzwansky, K.B. 2001. J. Immunol.166, 5550. Burkhardt, M., et al. 2000. J. Biol. Chem.275, 33536. Harbeck, B., et al. 2000. J. Biol. Chem.275, 40. Smolenski, A., et al. 2000. J. Biol. Chem.275, 25723.
Data Sheet
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Detection of human VASP, phosphorylated on Ser157, by immunoblotting. Samples: Whole cell extracts from human platelets cells left untreated (lane 1) or SNP treated cells (lane 2). Primary antibody: PhosphoDetect™ Anti-VASP (pSer157) Mouse mAb (5C6) (Cat. No. 676604) (0.5 µg/ml). Detection: chemiluminescence.
Description
Mouse monoclonal antibody purified from serum-free cell culture supernatant by thiophilic adsorption and size exclusion chromatography. Recognizes the the ~50 kDa VASP protein phosphorylated at Ser157.
Background
VASP (vasodilator-stimulated phosphoprotein), an established substrate of cAMP- and cGMP-dependent protein kinases in vitro and in living cells, is associated with focal adhesions, microfilaments, and membrane regions of high dynamic activity. VASP binds in vitro to profilin, zyxin, vinculin, and the Listeria sp. surface protein ActA. VASP also promotes actin nucleation and binds to actin filaments in vitro and associates with stress fibers in cells. Studies imply that VASP phosphorylation may act as an immediate negative regulator of actin dynamics. Phosphorylation of Ser157 in VASP can be used as a measure of PKA activity.
Host
Mouse
Immunogen species
Human
Immunogen
a synthetic phosphopeptide corresponding to amino acids surrounding the Ser¹⁵⁷ phosphorylation site of human VASP
Clone
5C6
Isotype
IgG₁
Species
human, mouse
Positive control
Vanadate-treated A431 cells
Form
Lyophilized
Formulation
Lyophilized from PBS, PEG, sucrose.
Preservative
≤0.1% sodium azide
Comments
Does not cross-react with non-phosphorylated VASP or with unrelated phosphorylation sites. For immunocytochemistry, fixation protocol may include up to 0.5% formaldehyde. Variables associated with assay conditions will dictate the proper working dilution.
Storage
-20°C
Do Not Freeze
Ok to freeze
Special Instructions
Reconstitute in 1 ml sterile, dH₂O, 15 min, room temperature. Following reconstitution, refrigerate (4°C) for short-term storage or aliquot, flash-freeze in liquid nitrogen, and freeze (-70°C) for long-term storage. Stock solutions are stable for up to 3 months at 4°C or for up to 1 year at -70°C. Thaw aliquots at 37°C. Avoid freeze/thaw cycles of solutions.
Toxicity
Standard Handling
References
Bear, J.E., et al. 2001. Curr. Opin. Cell. Biol.13, 158. Lawrence, D.W., and Pryzwansky, K.B. 2001. J. Immunol.166, 5550. Burkhardt, M., et al. 2000. J. Biol. Chem.275, 33536. Harbeck, B., et al. 2000. J. Biol. Chem.275, 40. Smolenski, A., et al. 2000. J. Biol. Chem.275, 25723.
