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70663 Proteinase K, Lyophilized

Highly active serine protease that exhibits broad cleavage specificity on native and denatured proteins and is widely used in the purification of DNA and RNA.

Proteinase K, Lyophilized MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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70663
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Overview

Replacement Information

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70663-4
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      		 Plastic ampoule 100 mg
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      70663-5
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          		 Glass bottle 500 mg
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          Description
          OverviewProteinase K is a highly active 28,904-Da serine protease isolated from the fungus Tritirachium album. The enzyme exhibits broad cleavage specificity on native and denatured proteins and is widely used in the purification of DNA and RNA. Its activity is increased in the presence of denaturants such as SDS (1%) and elevated temperature (50-60°C). The recommended working concentration is 50-100 µg/ml for protein removal and enzyme inactivation, and up to 2 mg/ml for tissue treatment. The Proteinase K, Lyophilized powder can be prepared as a 20 mg/ml stock solution in water and stored in aliquots at -20°C. The enzyme is also available as a ready-to-use concentrated stock solution (600 mAU/ml) that is convenient for routine use in most applications. 1 mg of Proteinase K is the equivalent of 30 mAU (AU = Anson unit). The Novagen Proteinase K products are free of detectable DNase and RNase
          Catalogue Number70663
          Brand Family Novagen®
          References
          Product Information
          CAS number39450-01-6
          Unit of DefinitionOne AU (AU = Anson unit) is defined as the amount of enzyme that liberates 1.0 µmol (181 µg) of tyrosine from casein per minute at pH 7.5 at 37°C.
          Quality LevelMQ400
          Applications
          Biological Information
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          R PhraseR: 36/37/38-42

          Irritating to eyes, respiratory system and skin.
          May cause sensitization by inhalation.
          S PhraseS: 22-24-26-36/37

          Do not breathe dust.
          Avoid contact with skin.
          In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
          Wear suitable protective clothing and gloves.
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Shipped with Blue Ice or with Dry Ice
          Toxicity Harmful
          Storage +2°C to +8°C
          Do not freeze Ok to freeze
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications
          Global Trade Item Number
          Catalog Number GTIN
          70663-4 04055977257670
          70663-5 07790788059859

          Documentation

          Proteinase K, Lyophilized SDS

          Title

          Safety Data Sheet (SDS) 

          Proteinase K, Lyophilized Certificates of Analysis

          TitleLot Number
          70663

          Citations

          Title
        • Shannon M. Anderson, et al. (2007) New markers for murine memory B cells that define mutated and unmutated subsets. Journal of Experimental Medicine 204, 2103-2114.
        • Andrew A. Horwitz, et al. (2007) ATP-induced structural transitions in PAN, the proteasome-regulatory ATPase complex in Archaea. Journal of Biological Chemistry 282, 22921-22929.
          		•

          User Protocols

          Title
          TB271 Proteinase K

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          Categories

          Life Science Research > Proteins and Enzymes > Other Enzymes
          Life Science Research > Genomic Analysis > DNA Preparation & Cloning > DNA Purification > DNA/RNA Clean-Up