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71772 ProteoExtract® Transmembrane Protein Extraction Kit

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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71772
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Overview

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71772-3
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      		 20 rxn
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      Description
      OverviewThe ProteoExtract® Transmembrane Protein Extraction Kit (TM-PEK) provides a detergent-free novel chemistry to enable the mild and efficient extraction of transmembrane proteins, such as GPCRs, from mammalian cells and tissues. The fraction enriched in membrane proteins and large protein complexes is directly compatible with enzyme assays, non-denaturing and SDS-based gel electrophoresis, Western blotting and immunoprecipitation. The enriched fraction is appropriate for mass spec-based analysis after a tryptic in-gel digest. The kit contains two different extraction reagents for protein-dependent optimization, protease inhibitor cocktail, and appropriate buffers for processing twenty samples (2-5 x 106 cells or 25-50 mg tissue per sample).
      Catalogue Number71772
      Brand Family Novagen®
      References
      ReferencesNakamura, E., et al. 2008. Cancer Sci. 99, 1390.
      Product Information
      Applications
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      R PhraseR: 25-33

      Toxic if swallowed.
      Danger of cumulative effects.
      S PhraseS: 37-45

      Wear suitable gloves.
      In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible).
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Toxic
      Storage +2°C to +8°C
      Do not freeze Ok to freeze
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      71772-3 04055977250909

      Documentation

      ProteoExtract® Transmembrane Protein Extraction Kit Certificates of Analysis

      TitleLot Number
      71772

      References

      Reference overview
      Nakamura, E., et al. 2008. Cancer Sci. 99, 1390.

      Citations

      Title
    • Waetzig G.H., et al. 2010. The Journal of Biological Chemistry 285, 1781.
    • Ernst, C.M., et al. 2009. doi: 10.1371/journal.ppat.1000660
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      User Protocols

      Title
      TB477