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CC300 PureCol®-S, Collagen Standard Solution

Ready-to-use ultrapure collagen type I protein for cell culture applications (20 mL).

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CC300
20 mL  
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      Overview

      Replacement Information
      Description
      Catalogue NumberCC300
      DescriptionPureCol®-S, Collagen Standard Solution
      OverviewCollagen is the main structural protein of the extracellular matrix found within various connective tissues of the body. Type I collagen is a major structural component of skin, bone, tendon, and other fibrous connective tissues. In order to provide the most in vivo-like environment for cells, researchers have historically cultured cells inside collagen hydrogels or on top of collagen coated plasticware.
      PureCol® is an ultrapure bovine skin derived collagen that is approximately 97% Type I collagen with the remainder being comprised of Type III collagen. It contains a high monomer content as measured by gel permeation chromatography providing product consistency, reproducibility and is cited in over 2000 publications.
      PureCol®-S is a soluble atelocollagen in 0.01 N HCI with a pH of 1.9-2.3. PureCol®-S is sterile filtered and is supplied as a ready-to-use solution for cell culture. This product is supplied at 3 mg/mL and can be used as a standard in cell culture applications such as cell adhesion and expansion.

      Instructions For Use

      Coating Procedure

      Note: Use these recommendations as guidelines to determine the optimal coating conditions for your culture system.

      1. Remove required quantity of PureCol®-S from the bottle and dispense into a dilution vessel.
      2. Dilute PureCol®-S in water to ~50 to 100 µg/ml (~1:30). A 0.01 N HCl solution may also be used.
      3. Swirl contents gently until material is completely mixed.
      4. Add appropriate amount of diluted PureCol®-S material to the culture surface ensuring that the entire surface is coated.
      5. Incubate at room temperature, covered, for 1-2 hours. Aspirate any remaining material. Alternatively, incubate at room temperature until surface is dry.
      6. Rinse coated surfaces carefully with sterile medium or PBS, avoid scratching surfaces.
      7. Coated surfaces are ready for use. They may also be stored at 2-8°C damp or air dried if sterility is maintained.

      3D Gel Preparation Procedure

      1. Slowly add 1 part of chilled 10X PBS or 10X culture media to 8 parts of chilled collagen solution with gentle swirling.
      2. Adjust pH of mixture to 7.2–7.6 using sterile 0.1 M NaOH. Monitor pH adjustment carefully (pH meter, phenol red, or pH paper).
      3. Adjust final volume to a total of 10 parts with sterile water.
      4. To prevent gelation, maintain temperature of mixture at 2–10°C.
      5. To form gel, warm to 37°C. Allow approximately 90 to 120 minutes for gel formation.

      PureCol® is a registered trademark of Advanced BioMatrix, Inc.
      Alternate Names
      • Collagen Type I
      References
      Product Information
      Applications
      ApplicationReady-to-use ultrapure collagen type I protein for cell culture applications (20 mL).
      Key Applications
      • Cell Culture
      • Cell Attachment
      Biological Information
      Concentration2.9-3.2 mg/mL
      HostBovine
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceCollagen Concentration: 2.9-3.2 mg/mL
      Collagen Concentration - AAA FIO: 2.9-3.2 mg/mL
      Sterility (USP modified): No Growth
      Endotoxin LAL: ≤ 1.0 EU/mL
      Gel Formation Tube Test: ≤ 40 minutes
      Kinetic Gel Test: ≤ 40 minutes
      Purity – SDS Page Silver Stain: ≥ 99.9%
      pH: 1.9-2.3
      Osmolality: ≤ 35 mOsm/Kg H20
      Fibrillogenesis: ≥ 0.5 Absorbance Units
      Electrophoretic Pattern: Characteristic
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore the PureCol®-S, Collagen Standard Solution at 2-8°C. Aliquot into smaller working aliquots before use to ensure product integrity and performance.
      Packaging Information
      Material Size20 mL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      CC300 04061841868675