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14-1058 PvuRts1I (5-hmC Restriction Endonuclease)

PvuRts1I is an enzyme that can specifically digest DNA containing 5-hydroxymethylcytosine (5-hmC). This restriction endonucleaseis used to analyze 5-hmC patterns genomic DNA by cleaving both glucosylated & non-glucosylated 5-hmC DNA sequences.

PvuRts1I (5-hmC Restriction Endonuclease) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Enzyme Assays
14-1058
200 Units  
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      Overview

      Replacement Information

      Key Specifications Table

      Key Applications
      Enzyme Assays
      Description
      Catalogue Number14-1058
      DescriptionPvuRts1I (5-hmC Restriction Endonuclease)
      OverviewSpecifications
      • Enzme Recognition Site: hmCN11-12/N910G
      • Unit Definition: 1 Unit will digest 1µg of hmC-containing DNA to completion in 20 minutes at room temperature in 1X reaction buffer.
      • Purity >95% purity by SDS PAGE
      • Source: Proteus vulgaris
      • Host: E. coli
      Alternate Names
      • PvuRts
      • Pvu
      Background InformationPvuRts1I is an enzyme isolated from the gram negative bacterium Proteus vulgaris that can specifically digest DNA containing 5-hmC (Szwagierczak et al., Nucleic Acids Research, 2011) while leaving cytosine and 5-mC intact. This endonuclease can be used to analyze 5-hmC patterns in mammalian genomes by cleave cutting both glucosylated and non-glucosylated 5-hmC containing DNA sequences.

      Research studies of DNA methylation and gene expression support the presence of 5-hydroxymethylcytosine (5-hmC) as a sixth base in mammalian genomes. 5-hmC is generated by Ten Eleven Translocation (TET) family of enzymes through oxidation of 5-methylcytosine (5-mC). Chemically, 5-hmC differs from 5-mC by the presence of a hydroxyl on the methyl group at position 5 of cytosine.
      References
      Product Information
      PresentationMaterials Provided:
      PvuRts1I 5-hmC Endonuclease: 100U (1U/µL) in a buffer containing 20mM Tris pH 8.0, 150mM NaCl, 1mM DTT and 50% Glycerol.

      PvuRts1I 5-hmC 10X Reaction Buffer: 500µL of buffer containing 200mM Tris pH 8.0, 1.5M NaCl, 50mM MgCl2 and 10mM DTT.
      Quality LevelMQ100
      Applications
      ApplicationPvuRts1I is an enzyme that can specifically digest DNA containing 5-hydroxymethylcytosine (5-hmC). This restriction endonucleaseis used to analyze 5-hmC patterns genomic DNA by cleaving both glucosylated & non-glucosylated 5-hmC DNA sequences.
      Key Applications
      • Enzyme Assays
      Biological Information
      HostE. coli
      PurityGreater than 95% purity by SDS PAGE
      Molecular Weight35 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable at -20ºC for 1 year from date of receipt.
      Packaging Information
      Material Size200 Units
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      14-1058 04053252941047

      Documentation

      PvuRts1I (5-hmC Restriction Endonuclease) Certificates of Analysis

      TitleLot Number
      PvuRts1I - QVP1310031 QVP1310031
      PvuRts1I - VP2001100 VP2001100
      PvuRts1I - VP2210040 VP2210040
      PvuRts1I - VP2410070 VP2410070
      PvuRts1I -VP1506072 VP1506072
      PvuRts1I 5-hmC Endonuclease VP1701072
      PvuRts1I 5-hmC Endonuclease - VP2203092 VP2203092

      References

      Reference overviewPub Med ID
      Characterization of PvuRts1I endonuclease as a tool to investigate genomic 5-hydroxymethylcytosine.
      Szwagierczak, Aleksandra, et al.
      Nucleic Acids Res., 39: 5149-56 (2011)  2011

      Show Abstract       		21378122 21378122

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      Categories

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