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GF240 Sema3A Recombinant Protein

Sema3A Recombinant Protein MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
UniProt Number: Q14563
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GF240
10ug  
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      Overview

      Replacement Information
      Description
      Catalogue NumberGF240
      DescriptionSema3A Recombinant Protein
      Background InformationSemaphorins are a large family of well conserved proteins that participate in a variety of cell signaling functions. However, they are best known for their role in development and regeneration within the nervous system. These affects have been shown to be primarily mediated by the class 3 semaporins (Sema3). In particular, the Sema3A family member acts as both a chemorepellant for axonal growth and a chemoattractant for dendritic processes1,2.

      Here we provide recombinant Sema3A protein to facilitate researchers studying neurite outgrowth. The protein produced is an Fc-chimera containing the highly conserved semphorin domain, the Ig-like domain, and the highly positively charged C-terminal region (without the 20 amino acids at the C-terminus)3.
      References
      Product Information
      PresentationProtein A affinity purified recombinant Sema3A protein in PBS buffer.
      Quality LevelMQ100
      Applications
      Biological Information
      Concentration0.25 mg/mL
      PurityThe purity of the Sema3A protein was also determined by SDS-PAGE. Purified protein (2µg) was run using non-denaturing conditions and then coomassie blue stained. The protein detected was approximately 125kD in size.
      Species Reactivity
      • Chicken
      • Human
      • Mouse
      • Rat
      Gene Symbol
      • Hsema-I
      • Hsema-III
      • SEMA1
      • SEMAD
      • SEMAIII
      • SEMAL
      • SemD
      • coll-1
      • semaphorin-like
      Purification Method40µl of 0.25mg/ml recombinant protein.
      UniProt Number
      UniProt SummaryFUNCTION: Induces the collapse and paralysis of neuronal growth cones. Could serve as a ligand that guides specific growth cones by a motility-inhibiting mechanism. Binds to the complex neuropilin-1/plexin-1 By similarity.

      SUBCELLULAR LOCATION: SecretedBy similarity.

      DOMAIN: Strong binding to neuropilin is mediated by the carboxy third of the protein.

      SEQUENCE SIMILARITIES: Belongs to the semaphorin family.

      Contains 1 Ig-like C2-type (immunoglobulin-like) domain.

      Contains 1 PSI domain.

      Contains 1 Sema domain.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceGrowth cone collapse testing was carried out to verify recombinant Sema3A protein induces collapse. Briefly, dorsal root ganglion cells were plated and allowed to grow overnight in differentiation media. The next day the media was replaced with differentiation media or differentiation media with 1µg/ml of Sema3A protein. The cells were then incubated for 30 minutes, fixed, and stained with FITC conjugated antibodies specific for phalloidin (Part No. 90228). Representative images of each assay condition are shown below:
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore at -20°C or at -80°C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
      Packaging Information
      Material Size10ug
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      GF240 04053252474934

      Documentation

      Sema3A Recombinant Protein SDS

      Title

      Safety Data Sheet (SDS) 

      Sema3A Recombinant Protein Certificates of Analysis

      TitleLot Number
      Sema3A Recombinant Protein 2929764
      Sema3A Recombinant Protein 2444303
      Sema3A Recombinant Protein 2772940
      Sema3A Recombinant Protein - 2168274 2168274
      Sema3A Recombinant Protein - 3387289 3387289
      Sema3A Recombinant Protein - 3704570 3704570
      Sema3A Recombinant Protein - 3865148 3865148
      Sema3A Recombinant Protein - 4187774 4187774
      Sema3A Recombinant Protein -2550611 2550611