688000 Sigma-AldrichY-27632 - CAS 331752-47-7 - Calbiochem
Y-27632, CAS 146986-50-7, is a cell-permeable, reversible, inhibitor of Rho kinases (Ki = 140 nM for p160ROCK). Enhances survival & cloning efficiency of ESC without affecting their pluripotency.
More>> Y-27632, CAS 146986-50-7, is a cell-permeable, reversible, inhibitor of Rho kinases (Ki = 140 nM for p160ROCK). Enhances survival & cloning efficiency of ESC without affecting their pluripotency. Less<<Y-27632 - CAS 331752-47-7 - Calbiochem MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Synonyms: (R)-(+)-trans-N-(4-Pyridyl)-4-(1-aminoethyl)-cyclohexanecarboxamide, 2HCl, ROCK Inhibitor, Rho Kinase Inhibitor VI
Recommended Products
Overview
| Replacement Information |
|---|
Key Specifications Table
| CAS # | Empirical Formula |
|---|---|
| 331752-47-7 | C₁₄H₂₁N₃O · 2HCl · H₂O |
Pricing & Availability
| Catalog Number | Availability | Packaging | Qty/Pack | Price | Quantity | |
|---|---|---|---|---|---|---|
| 688000-100MG |
|
Plastic ampoule | 100 mg |
|
— | |
| 688000-10MG |
|
Glass bottle | 10 mg |
|
— | |
| 688000-1MG |
|
Plastic ampoule | 1 mg |
|
— | |
| 688000-5MG |
|
Glass bottle | 5 mg |
|
— |
| Description | |
|---|---|
| Overview | A highly potent, cell-permeable, reversible, and selective inhibitor of Rho-associated protein kinases (Ki = 140 nM for p160ROCK). Also inhibits ROCK-II with equal potency. The inhibition is competitive with respect to ATP. Exhibits 10- to 50-fold lower affinity for PKCε than p160ROCK. Does not affect the activity of p21-activated protein kinase (PAK) even at higher concentrations (~100 µM). Also acts as a potent inhibitor of agonist-induced Ca2+ sensitization of myosin phosphorylation and smooth muscle contraction. Prevents apoptosis and enhances the survival and cloning efficiency of dissociated hES cells without affecting their pluripotency. Pretreatment with Y-27632 reduces the risk of lung injury in rat models of sepsis. A 5 mM (500 µg/296 µl) solution of Y-27632 (Cat. No. 688001) in H2O and a 10 mM solution in DMSO (Cat. No. 688002) are also available. |
| Catalogue Number | 688000 |
| Brand Family | Calbiochem® |
| Synonyms | (R)-(+)-trans-N-(4-Pyridyl)-4-(1-aminoethyl)-cyclohexanecarboxamide, 2HCl, ROCK Inhibitor, Rho Kinase Inhibitor VI |
| Product Information | |
|---|---|
| CAS number | 331752-47-7 |
| ATP Competitive | Y |
| Declaration | Sold under license of PCT Application W098/06,433A1 and under license from Mitsubishi Pharma Tanabe Corporation. |
| Form | White solid |
| Hill Formula | C₁₄H₂₁N₃O · 2HCl · H₂O |
| Chemical formula | C₁₄H₂₁N₃O · 2HCl · H₂O |
| Hygroscopic | Hygroscopic |
| Reversible | Y |
| Structure formula Image | |
| Quality Level | MQ100 |
| Biological Information | |
|---|---|
| Primary Target | p160 Rho-associated protein kinases (ROCK) |
| Primary Target K<sub>i</sub> | 140 nM for p160 Rho-associated protein kinases (ROCK) |
| Purity | ≥95% by HPLC |
| Physicochemical Information | |
|---|---|
| Cell permeable | Y |
| Dimensions |
|---|
| Materials Information |
|---|
| Toxicological Information |
|---|
| Safety Information according to GHS |
|---|
| Safety Information | |
|---|---|
| R Phrase | R: 20/21/22 Harmful by inhalation, in contact with skin and if swallowed. |
| S Phrase | S: 36 Wear suitable protective clothing. |
| Product Usage Statements |
|---|
| Packaging Information | |
|---|---|
| Packaged under inert gas | Packaged under inert gas |
| Transport Information |
|---|
| Supplemental Information |
|---|
| Specifications |
|---|
| Global Trade Item Number | |
|---|---|
| Catalog Number | GTIN |
| 688000-100MG | 04055977225402 |
| 688000-10MG | 04055977225396 |
| 688000-1MG | 07790788052362 |
| 688000-5MG | 07790788052379 |
Documentation
Y-27632 - CAS 331752-47-7 - Calbiochem SDS
| Title |
|---|
Y-27632 - CAS 331752-47-7 - Calbiochem Certificates of Analysis
| Title | Lot Number |
|---|---|
| 688000 |
References
| Reference overview |
|---|
| Cinel, I., et al. 2012. J. Thorac . Dis. 4, 30. Watanabe, K., et al. 2007. Nature Biotech. 25, 681. Chitaley, K., et al. 2001. Nat. Med. 7, 119. Davies, S.P., et al. 2000. Biochem. J. 351, 95. Narumiya, S., et al. 2000. Methods Enzymol. 325, 273. Hirose, M., et al. 1998. J. Cell Biol. 141, 1625. Maekawa, M., et al. 1999. Science 285, 895. Uehata, M., et al. 1997. Nature 389, 990. |
Citations
| Title | |
|---|---|
|
|
| Data Sheet | ||||||||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
|
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
|