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MAB5374

Sigma-Aldrich

Anti-Huntingtin Disease (HD/HTT) Antibody

CHEMICON®, mouse monoclonal, mEM48

Synonym(s):

Huntingtin

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100 μL
$635.00

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100 μL
$635.00

About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

$635.00

Check Cart for Availability
A recombinant, preservative-free antibody is available for your target. Try ZRB1102
Request a Bulk Order

Product Name

Anti-Huntingtin Protein Antibody, clone mEM48, culture supernatant, clone mEM48, Chemicon®

biological source

mouse

Quality Level

100

antibody form

culture supernatant

antibody product type

primary antibodies

clone

mEM48, monoclonal

species reactivity

human

species reactivity (predicted by homology)

mouse, rat

manufacturer/tradename

Chemicon®

technique(s)

immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable

isotype

IgG

NCBI accession no.

NM_002111.6

UniProt accession no.

P42858

target post-translational modification

unmodified

Gene Information

human ... HTT(3064), SLC6A4(6532)

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P1874H7540MAB2170
Anti-Huntingtin Protein Antibody, clone mEM48 culture supernatant, clone mEM48, Chemicon®

MAB5374

Anti-Huntingtin Protein Antibody, clone mEM48

Anti-Polyglutamines antibody, Mouse monoclonal ~2 mg/mL, clone 3B5H10, purified from hybridoma cell culture

P1874

Anti-Polyglutamines antibody, Mouse monoclonal

Anti-Huntingtin (N-terminal) antibody produced in rabbit affinity isolated antibody, buffered aqueous solution

H7540

Anti-Huntingtin (N-terminal) antibody produced in rabbit

Anti-Huntingtin Protein Antibody, a.a. 1247-1646, clone HU-4E6 ascites fluid, clone HU-4E6, Chemicon®

MAB2170

Anti-Huntingtin Protein Antibody, a.a. 1247-1646, clone HU-4E6

species reactivity

human

species reactivity

human

species reactivity

mouse, human, rat

species reactivity

mouse, human

biological source

mouse

biological source

mouse

biological source

rabbit

biological source

mouse

Gene Information

human ... HTT(3064), SLC6A4(6532)

Gene Information

human ... HTT(3064)

Gene Information

human ... HTT(3064)
mouse ... Htt(15194)
rat ... Htt(29424)

Gene Information

human ... HTT(3064), SLC6A4(6532)

clone

mEM48, monoclonal

clone

3B5H10, monoclonal

clone

polyclonal

clone

HU-4E6, monoclonal

antibody form

culture supernatant

antibody form

purified from hybridoma cell culture

antibody form

affinity isolated antibody

antibody form

ascites fluid

technique(s)

immunocytochemistry: suitable, western blot: suitable, immunohistochemistry: suitable

technique(s)

immunocytochemistry: suitable, immunoprecipitation (IP): suitable, western blot: 1-2 μg/mL using extract of HEK-293T cells transfected with an N-terminal 171 amino acid fragment of human Huntingtin with a 68 glutamine stretch

technique(s)

western blot: 0.5-1.0 μg/mL using a HEK-293T cell lysate expressing a N-terminal fragment of human huntingtin

technique(s)

ELISA: suitable, immunocytochemistry: suitable, immunohistochemistry: suitable (paraffin), immunoprecipitation (IP): suitable, western blot: suitable

General description

Huntington disease (HD) is a hereditary, progressive, neurodegenerative ailment characterized by personality changes, motor impairment and subcortical dementia. The molecular basis of the disease involves the expansion of the trinucleotide CAG, coding for polyglutamine in the first exon of a chromosome four gene (4p16.3), which normally produces a widely expressed 3136 a.a. (~350 kDa) protein huntingtin with unclear function. The protein is found in the perinuclear region along with microtubules, and in the centrosomal region along with gamma-tubulin. Huntingtin is necessary for neuronal survival and is involved in synaptic vesicle trafficking, microtubule binding and may also have a role in apoptosis. In the HD condition, neuronal cells with the mutant form of huntingtin possess intranuclear aggregations of the N-terminal fragment, causing damaging inclusions in perinuclear locations and striatal neuron cell death. Wild-type huntington and anti-huntingtin reduce aggregation and cellular toxicity of the mutant huntingtin form in mammalian cell models of HD. Huntingtin is known to interact with GAPDH, HAP-1, SP1 and TAFII130.

Specificity

Reacts with human huntingtin protein (both native and recombinant protein). MAB5374 reacts with mutant huntingtin in patients and in transgenic animals that express different numbers of repeats (from 82 to 150 glutamines). Thus, it should recognize different forms of mutant huntingtin.

Immunogen

GST fusion protein from the first 256 amino acids from human huntingtin with the deletion of the polyglutamine tract.

Application

Detect Huntingtin Protein using this Anti-Huntingtin Protein Antibody, clone mEM48 validated for use in IC, IH & WB.
Immunohistochemistry:
1:50-1:100 of a previous lot using ABC on 4% paraformaldehyde fixed tissue. Suggested dilution buffer is PBS containing 3% BSA. The antibody works on paraffin embedded tissue sections.
Suggested dilution buffer is PBS containing 3% BSA. The antibody works on paraffin embedded tissue sections. Yu, Z et al (2002) Hum. Mole. Genetics 11(8):905-914. (http://hmg.oxfordjournals.org/cgi/content/full/11/8/905) for good IHC methods and photos of mEM48 on rodent tissues with human transgenic material.

Immunocytochemistry:
light 4% PFA fixation followed by 0.1% triton X-100 incubation prior to blocking is suggested.
A previous lot of this antibody was used in IC.

Western blot:
1:50-1:500 using ECL depending on the level of mutant protein. Suggested dilution buffer is PBS containing 3% BSA or PBS containing 5% non-fat milk.
Nuclear fraction preparations enhance signals; monomeric protein ~80kDa; aggregates are common which can be >200kDa in size.

Optimal working dilutions must be determined by the end user.
Research Category
Neuroscience
Research Sub Category
Neurodegenerative Diseases

Quality

Routinely evaluated by Western Blot on HEK293 lysates.

Western Blot Analysis:
1:1000 dilution of this lot detected Huntingtin Protein on 10 μg of HEK293 lysates.

Target description

over 200 kDa

Physical form

Culture Supernatant mouse monoclonal IgG containing no preservative.
Unpurified

Storage and Stability

Stable for 6 months at -20ºC in undiluted aliquots from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analysis Note

Control
Normal human cerebral cortex lysate, mouse brain cortex samples from HD or wild type mice

HEK293 lysates.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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