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antibody form
purified antibody
clone
E16
monoclonal
purified by
using protein G
species reactivity
virus, mouse
concentration
(Please refer to lot specific datasheet.)
technique(s)
immunocytochemistry: suitable
western blot: suitable
isotype
IgG2bκ
capacity
neutralization
UniProt accession no.
General description
West Nile Virus envelope protein.
Specificity
Clone E16 is a mouse monoclonal antibody that specifically detects envelope protein of West Nile Virus. It targets an epitope within the DIII lateral ridge region.
Application
Immunocytochemistry Analysis: A representative lot detected West Nile Virus in Immunocytochemistry applications (Thompson, B.S., et. al. (2009). PLoS Pathog. 5(5):e1000453).
Neutralizing Analysis: A representative lot completely inhibited West Nile virus infection at 10 ng/mL. (Oliphant, T., et. al. (2006). J Virol. 80(24):12149-59; Thompson, B.S., et. al. (2009). PLoS Pathog. 5(5):e1000453).
Neutralizing Analysis: A representative lot completely inhibited West Nile virus infection at 10 ng/mL. (Oliphant, T., et. al. (2006). J Virol. 80(24):12149-59; Thompson, B.S., et. al. (2009). PLoS Pathog. 5(5):e1000453).
Quality
Evaluated by Western Blotting with Full-length ectodomain of West Nile Virus envelope protein.
Western Blotting Analysis: 1 µg/mL of this antibody detected Full-length ectodomain of West Nile Virus envelope protein.
Western Blotting Analysis: 1 µg/mL of this antibody detected Full-length ectodomain of West Nile Virus envelope protein.
Physical properties
~50 kDa observed; 31.88 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physical form
Purified mouse monoclonal antibody IgG2b in PBS without azide.
Storage and Stability
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Other Notes
Virus.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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