Cell quantitation and analysis techniques such as hemocytometry that are based on image analysis have limited sensitivity and objectivity, and sample sizes may be too small for statistical analysis. Muse® assays use relative fluorescence and flow cytometry to assess thousands of cells per sample, rendering data that is more accurate, more precise, and more reliable than traditional methods.
Enhanced Sensitivity + More Data Per Sample + Stable Assays = Superior Reliability
More Accurate Cell Analysis
The Muse® Cell Analyzer counts cells more accurately than manual hemocytometry or image-based automated analysis. Multiple adherent and suspension cell types were harvested, diluted and counted. Cell counts from all three methods were averaged to obtain "theoretical cell concentration". Each point represents the average cell concentration of 3 replicates, and each data series was fit with linear regression. Muse® cell analysis data were correlated with the theoretical concentration with slope closest to 1, indicating superior accuracy.
More Precise Cell Analysis
The Muse® Cell Analyzer counts cells and measures viability more precisely with smaller coefficients of variation (%CV) than manual hemocytometry or image-based automated analysis. When compared with image-based automated counting methods and manual hemocytometers, the Muse® Cell Analyzer exhibited a narrower range of %CVs and consistently provided variation of less than 10% over the entire range of samples tested. Higher %CVs were observed for the Trypan blue-based methods, particularly at lower cell concentrations.
| Analysis | Cell Concentration | Viability |
|---|
| Average % CV | % CV Range | Average % CV | % CV Range |
|---|
| Image-based Automated Counter |
9.2% |
1.2-23.3% |
3.7% |
0.8-12.1% |
| Manual Hemocytometer |
6.3% |
0.5-15.3% |
4.5% |
0.5-9.2% |
| Muse® |
4.0% |
0.3-8.8% |
2.2% |
0.4-5.6% |
The Muse® Cell Analyzer provides superior precision for cell concentration and viability measurements, compared to Trypan blue-based analyses. Data are based on triplicate measurements of 30 cellular samples from suspension and adherent cell lines at multiple concentrations and viabilities.
More Reliable Cell Analysis
The Muse® Cell Analyzer performs with exceptional fidelity across multiple cell lines and a wide sample concentration range. The concentration measurements for serial dilutions of five representative cell lines are shown, and include both adherent and suspension cells. Each point represents the average of three replicates. Expected cell concentrations were calculated by measuring the stock sample concentration with the Muse® Cell Analyzer, then dividing by the dilution factor to obtain theoretical concentrations of diluted samples. Data show the comparison of observed vs. expected cell concentrations.
