Recommended Working Volumes

Single-well, 24-well and 96-well inserts are available to meet a range of application needs. The protocols printed in this section of the guide can be adjusted to accommodate a range of platforms. Please use the following table as a reference for scaling liquid additions in Millicell protocols.

	Well Diameter (mm)	Membrane Surface Area (cm2)	Apical Volume (ml)	Basolateral Volume (ml) (24- or 96-well Receiver Tray)	Basolateral Volume (ml) (single-well Receiver Tray)
6-well standing inserts	30	4.2	1.5	2.0	n/a
6-well hanging inserts	27	4.5	2.0	4.2*	n/a
12-well hanging inserts	15	1.1	.4	2.0*	n/a
24-well standing inserts	12	.6	.4	.6	n/a
24-well hanging inserts	9	.3	.2	1.3*	n/a
Millicell-24 Cell Culture Insert Plate	11	.7	.3	.6	28
Millicell-96 Cell Culture Insert Plate	5	.1	.075	.25	32
MultiScreen-MIC Filter Plate	7	.3	.075	.15	n/a

* The basolateral volumes are measured in standard plastic culture plates with 18 mm well heights and are at equal head height with the apical well.

Recommended Working Volumes

Well Diameter (mm)

Membrane Surface Area (cm2)

Apical Volume (ml)

Basolateral Volume (ml) (24- or 96-well Receiver Tray)

Basolateral Volume (ml) (single-well Receiver Tray)

6-well standing inserts

6-well hanging inserts

Millicell-96 Cell Culture Insert Plate

MultiScreen-MIC Filter Plate