Analysis of a Sulfonamide Mixture on a Classical TLC Silica Gel 60 F254 Plate

Plate TLC silica gel 60 F254 (1.05715.0001) Merck:/Freestyle/LE-Lab-Essentials/Chromatography/Diagrams/LE-TLC and HPTLC-Chromatogram TLC Silica gel-480x710-03122014.jpg
Sample volume 0.75 μl
Mobile phase Ethyl acetate/methanol/ammonia solution 25%
(60/20/2) (v/v/v)
Detection UV 254 nm (TLC/HPTLC Scanner 2 CAMAG)
Compounds 1. Sulfadiazine
2. Sulfamerazine
3. Sulfisoxalozole
4. Sulfapyridine
5. Sulfanilamide (all 0.1%)