HPLC Grade Acids |
Acids Specfically for use in Preparing HPLC Buffers.
MilliporeSigma HPLC Acids are suitable for use in conjunction with HPLC analysis. Each HPLC grade acid meets ACS specifications* and includes testing for UV Absorbance and various trace metals impurities. HPLC Acids have the comprehensive specifications which guarantee laboratory personnel the quality assurance they demand. See below for a full listing of specifications for each of our three HPLC grade acids.
HPLC Acids
| Cat. No. | Product |
|---|---|
| AX0074 | Acetic Acid, Glacial |
| PX0996 | o-Phosphoric Acid |
| TX1276 | Trifluoroacetic Acid |
Acetic Acid, Glacial ACS-HPLC Grade
| Test | Specification |
|---|---|
| Assay | 99.7% Minimum |
| Acetic anhydride | 0.01% max |
| Color (APHA) | 10 max |
| Chloride | 0.4 ppm max |
| Copper | 0.1 ppm max |
| Dilution Test | To pass test |
| Heavy Metals (as Pb) | 0.5 ppm max |
| Iron | 0.2 ppm max |
| Nickel | 0.1 ppm max |
| Residue after evaporation | 5 ppm max |
| Substances reducing dichromate | To pass test |
| Substances reducing permanganate | To pass test |
| Sulfate | 0.4 ppm max |
| Titratable base | 0.0004 meq/g max |
| UV Absorbance (1-cm cell vs. water) |
0.02 AU max at 350nm 0.05 AU max at 280nm 1.0 AU max at 255nm |
| Water | 0.1% max |
o-Phosphoric Acid ACS-HPLC Grade
| Test | Specification |
|---|---|
| Assay | 85.0% Minimum |
| Antimony | 0.002% max |
| Arsenic | 0.5 ppm max |
| Color (APHA) | 10 max |
| Chloride | 3 ppm max |
| Heavy Metals (as Pb) | 0.001% ppm max |
| Insoluble matter, calcium, magnesium and ammonium hydroxide precipitate | 0.005% max |
| Iron | 0.003% max |
| Manganese | 0.5 ppm max |
| Nitrate | 5 ppm max |
| Phosphorous & Hypophosphorous acid | To pass test |
| Potassium | 0.005% max |
| Sodium | 0.025% max |
| Reducing substances | To pass test |
| Sulfate | 0.001% max |
| UV Absorbance (1 M, 1 cm, water) |
0.02 AU max at 300nm 0.04 AU max at 254nm 0.05 AU max at 220nm |
| Volatile acids (as CH3COOH) | 0.001% max |
Trifluoroacetic Acid HPLC Grade*
| Test | Specification |
|---|---|
| Assay | 99.9% Minimum |
| Chloride | 0.001% max |
| Color (APHA) | 5 max |
| Fluoride (F) | 0.001% max |
| Iron (FE) | 0.0001% max |
| Residue after evaporation | 0.0002% |
| Sulfate (SO4) | 0.001% |
| Trifluoroacetic anhydride | None detected |
| UV Absorbance (0.1%, 1 cm, water) | 0.15 AU max at 230nm 0.01 AU max at 254nm |
| Water | 0.05% max |
*No ACS specification for Trifluoroacetic acid