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Competent Cells

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For successful isolation and propagation of engineered DNA vectors, transformation into competent bacterial cells is a key step. The right strain of competent cells can make the difference between cloning frustration and progressing to the next step. MilliporeSigma offers a range of strains and formats to suit every cloning project. Since reliability is critical, we verify the phenotype and purity of each strain and guarantee its transformation efficiency. With more than two decades of experience producing competent cells for protein and molecular biology research, we offer robust performance you can count on.

NovaBlue™ Competent Cells

Transformation Efficiency*
Reaction Size
Application
GigaSingles™ > 1.0 x 109 cfu/µg 50 µL High-efficiency cloning
Singles™ > 1.5 x 108 cfu/µg 50 µL Routine cloning
Veggie™ Singles > 1.5 x 108 cfu/µg 50 µL

Applications requiring nonanimal-derived materials
Routine cloning
HT96™ > 1.0 x 108 cfu/µg 96 x 20 µL High-throughput cloning
Standard > 1.5 x 108 cfu/µg 20 µL Routine cloning

Veggie NovaBlue™ Singles Competent Cells

For cloning applications in which absence of animal-derived components is essential, use Veggie NovaBlue™ Singles Competent Cells. Like all NovaBlue™ competent cells, Veggie NovaBlue™ is a K-12 strain featuring high transformation efficiency, blue/white screening capability (with appropriate plasmids), and mutations in recA and endA, which result in excellent yields of high-quality plasmid DNA. Veggie NovaBlue™ Singles Competent Cells are supplied with an animal-free prepared SOC medium.

ColiRollers™ Plating Beads

Merck:/Freestyle/BI-Bioscience/Genomic-Analysis/colirollers.jpgColiRollers™ Plating Beads are specially treated glass beads that offer a better way to perform routine bacteria plating. The use of ColiRollers beads eliminates the inconsistencies of conventional "hockey stick" methods, and provides even distribution of bacterial colonies every time.