Amicon® Pro Purification System - Antibody Labeling |
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Label Antibodies Without Losing a Single Drop—
in as Little as One Hour.
- Read our 2015 paper in Journal of Immunological Methods
- Download application note on Complete Antibody Labeling in 1 hour
In contrast, the Amicon® Pro method requires just one hour. Also, thanks to the all-in-one format of the Amicon® Pro device, it eliminates loss due to sample transfers and can significantly improve recovery.
Buy the Amicon® Pro Purification System
Generate FITC-labeled antibody in one hour.
Compare the Amicon® Pro Workflow with Traditional Methods
Ab Labeling* | |||
|---|---|---|---|
| Process time | 3 days | 5 hours | 1 hour |
| Steps required | 11 | 8 | 5 |
| Sample transfers | 3 | 0 | 0 |
| Amount Ab required | 1 mg | 20 μg | 20 μg |
The antibody labeling protocol consists of four basic steps: antibody cleanup, the labeling reaction, unbound dye removal/buffer exchange, and concentration. The duration of the labeling reaction can vary dependent on individual reagent parameters. The process time presented here is based on biotinylation using the Innolink™ Biotin 354S kit (Cat. No. 203119, MilliporeSigma)