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565764 γ-Secretase Substrate, Fluorogenic - Calbiochem

An internally quenched fluorogenic peptide substrate containing the C-terminal β-APP (amyloid β-peptide precursor protein) amino acid sequence that is cleaved by γ-secretase.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: NMA-GGVVIATVK(DNP)-DRDRDR-NH₂

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565764
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565764-1MG
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      Description
      OverviewAn internally quenched fluorogenic peptide substrate containing the C-terminal β-APP (amyloid β-peptide precursor protein) amino acid sequence that is cleaved by γ-secretase. Shown to be sensitive and useful for assaying γ-secretase activity. The proteolysis at the Aβ40-, Aβ42-, and Aβ43-generating cleavage sites results in enhanced fluorescence.
      Catalogue Number565764
      Brand Family Calbiochem®
      SynonymsNMA-GGVVIATVK(DNP)-DRDRDR-NH₂
      References
      ReferencesFarmery, M.R., et al. 2003. J. Biol. Chem. 278, 24277.
      Product Information
      ATP CompetitiveN
      FormYellow Lyophilized
      FormulationSupplied as a trifluoroacetate salt.
      Hill FormulaC₇₀H₁₁₆N₂₆O₁₈
      Chemical formulaC₇₀H₁₁₆N₂₆O₁₈
      Hygroscopic Hygroscopic
      ReversibleN
      Quality LevelMQ100
      Applications
      Biological Information
      Primary Targetγ-secretase
      Purity≥95% by HPLC
      Physicochemical Information
      Cell permeableN
      Peptide SequenceNMA-Gly-Gly-Val-Val-Ile-Ala-Thr-Val-Lys(DNP)-D-Arg-D-Arg-D-Arg-NH₂
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage -20°C
      Protect from Light Protect from light
      Hygroscopic Hygroscopic
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot, flush with a stream of inert gas, and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
      Packaging Information
      Packaged under inert gas Packaged under inert gas
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      565764-1MG 04055977267730

      Documentation

      γ-Secretase Substrate, Fluorogenic - Calbiochem SDS

      Title

      Safety Data Sheet (SDS) 

      γ-Secretase Substrate, Fluorogenic - Calbiochem Certificates of Analysis

      TitleLot Number
      565764

      References

      Reference overview
      Farmery, M.R., et al. 2003. J. Biol. Chem. 278, 24277.

      Citations

      Title
    • Vorobyeva, A, et al. (2014) Cyclopamine Modulates Gamma-Secretase Mediated Cleavage of Amyloid Precursor Protein by Altering its Subcellular Trafficking and Lysosomal Degradation. J. Biol. Chemistry 289, 33258.
      		•
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision10-September-2008 RFH
      SynonymsNMA-GGVVIATVK(DNP)-DRDRDR-NH₂
      DescriptionAn internally quenched fluorogenic peptide substrate containing the C-terminal β-APP (amyloid β-peptide precursor protein) amino acid sequence that is cleaved by γ-secretase. Shown to be sensitive and useful for assaying γ-secretase activity. The proteolysis at the Aβ40-, Aβ42-, and Aβ43-generating cleavage sites results in enhanced fluorescence. Excitation max.: ~355 nm; emission max.: ~440 nm.
      FormYellow Lyophilized
      FormulationSupplied as a trifluoroacetate salt.
      Intert gas (Yes/No) Packaged under inert gas
      Recommended reaction conditions50 mM Tris-HCl, pH 6.8, 2 mM EDTA, 0.25% CHAPSO (w/v), 8 µM substrate, 37°C.
      Chemical formulaC₇₀H₁₁₆N₂₆O₁₈
      Peptide SequenceNMA-Gly-Gly-Val-Val-Ile-Ala-Thr-Val-Lys(DNP)-D-Arg-D-Arg-D-Arg-NH₂
      Purity≥95% by HPLC
      SolubilityDMSO (5 mg/ml) or 5% Acetic acid
      Storage Protect from light
      -20°C
      Hygroscopic
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot, flush with a stream of inert gas, and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
      Toxicity Standard Handling
      ReferencesFarmery, M.R., et al. 2003. J. Biol. Chem. 278, 24277.
      Citation
    • Vorobyeva, A, et al. (2014) Cyclopamine Modulates Gamma-Secretase Mediated Cleavage of Amyloid Precursor Protein by Altering its Subcellular Trafficking and Lysosomal Degradation. J. Biol. Chemistry 289, 33258.
      		•