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71194-M

Millipore

0.5 M THP Solution

Reducing agent compatible with His•Bind chromatography

Synonym(s):

Tris(3-hydroxypropyl)phosphine

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1 ML
$57.00
5 X 1 ML
$204.00

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1 ML
$57.00
5 X 1 ML
$204.00

About This Item

UNSPSC Code:
41106511
NACRES:
NA.77

$57.00

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Quality Level

400

form

solution

manufacturer/tradename

Novagen®

storage condition

OK to freeze

technique(s)

protein purification: suitable

application(s)

sample preparation

storage temp.

−20°C

General description

Reducing agent compatible with HIS-BIND® chromatography
THP [Tris(hydroxypropyl)phosphine; C9H21O3P] is a water-soluble, odorless, sulfhydryl reducing agent that is more stable and effective than dithiothreitol (DTT). Against oxidized glutathione, the reducing capacity of THP (0.232 meq/g) is greater than that of TCEP (0.182 meq/g). THP is also more resistant to air oxidation than DTT and is compatible with immobilized metal affinity chromatography with HIS-BIND® and Ni-NTA His•Bind resins at concentrations up to 1.0 mM. The common working concentration of THP for many applications is 15 mM. THP is provided as a ready-to-use 0.5 M solution in water.

Purity: >80% by NMR. CAS: 4706-17-6. MW: 208.2.

Features and Benefits

Compatability with common methods for purifying His-tagged proteins - immobilized metal affinity chromatography with HIS-BIND® and Ni-NTA His•Bind resins at concentrations up to 1.0 mM

Stability: More stable and effective than traditional reducing agents like dithiothreitol (DTT)

Reducing Capacity: greater reducing capacity than TCEP against oxidized glutathione

Ready-to-use: THP is provided as a ready-to-use 0.5 M solution in water, making it easy to use in many applications with flexible usage

Warning

Toxicity: Corrosive (L)

Other Notes

Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.

Legal Information

HIS-BIND is a registered trademark of Merck KGaA, Darmstadt, Germany
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

pictograms

Corrosion
GHS05

signalword

Danger

hcodes

H314

Hazard Classifications

Eye Dam. 1 - Skin Corr. 1B

Storage Class

8B - Non-combustible corrosive hazardous materials

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

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