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SCR005 MilliporeAccutase cell detachment solution

A cell detachment solution of proteolytic & collagenolytic enzymes. The reagent is useful for creating single cell suspensions from clumped cell cultures for accurate cell counting, detachment of cells from primary tissue.

Accutase cell detachment solution MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

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SCR005

100 mL

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Overview

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Description
Catalogue Number	SCR005
Brand Family	Chemicon®
Trade Name	Chemicon
Description	Accutase cell detachment solution
Overview	A cell detachment solution of proteolytic and collagenolytic enzymes. Useful for the routine detachment of cells from standard tissue culture plasticware and adhesion coated plasticware, including SmartPlastic™. ACCUTASE™ does not contain mammalian or bacterial derived products. Because the reagent does not contain universally proteolytic enzymes it may not release all cell/tissue types uniformly. Some optimization of cell type and passage number, as well as, incubation time and reagent strength typically needs to be performed to optimize the reagents effectiveness. ACCUTASE™ has been shown to be effective on a wide variety of cell types including: fibroblasts, keratinocytes, vascular endothelial cells, hepatocytes, vascular smooth muscle cells, hepatocyte progenitors, primary chick embryo neuronal cells, bone marrow stem cells, adherent CHO and BHK cells, macrophages, 293 cells, L929 cells, immortalized mouse testicular germ cells, 3T3, Vero, COS, HeLa, NT2, MG63, M24 and A375 metastatic melanoma, gliomas U251 and D54, HT1080 fibrosarcoma cells, and Sf9 insect cells. Each lot of ACCUTASE™ is tested for Sterility (by USP membrane filtration method), enzymatic activity (tested with synthetic chromagenic tetrapeptides) and cell detachment from tissue culture plastic.

References

Product Information
HS Code	3507 90 49
Presentation	100 ml, ready to use, frozen sterile filtered (0.2 micron) liquid. 1X ACCUTASE enzymes in Dulbecco's PBS (0.2 g/L KCl, 0.2 g/L KH2PO4, 8 g/L NaCl, and 1.15 g/L Na2HPO4) containing 0.5 mM EDTA·4Na and 3 mg/L Phenol Red.
Quality Level	MQ300

Applications
Application	A cell detachment solution of proteolytic & collagenolytic enzymes. The reagent is useful for creating single cell suspensions from clumped cell cultures for accurate cell counting, detachment of cells from primary tissue.
Application Notes	Cell Detachment 1. Thaw ACCUTASE™ at room temperature. 2. Wash plate, flask or beads with sterile PBS. 3. Add ACCUTASE™ to culture dish or flask using aseptic procedures at 10 ml per 75-cm2 surface area. 4. Return culture to 37°C incubator and allow cells to detach (5-10 minutes). 5. Count cells and passage as usual. No additional washes or enzyme inhibitors are required Optimal incubation times and reagent strength needs to be determined for each cell/tissue type tested by the enduser.

Applications

Application

A cell detachment solution of proteolytic & collagenolytic enzymes. The reagent is useful for creating single cell suspensions from clumped cell cultures for accurate cell counting, detachment of cells from primary tissue.

Application Notes

Cell Detachment

1. Thaw ACCUTASE™ at room temperature.

2. Wash plate, flask or beads with sterile PBS.

3. Add ACCUTASE™ to culture dish or flask using aseptic procedures at 10 ml per 75-cm2 surface area.

4. Return culture to 37°C incubator and allow cells to detach (5-10 minutes).

5. Count cells and passage as usual. No additional washes or enzyme inhibitors are required

Optimal incubation times and reagent strength needs to be determined for each cell/tissue type tested by the enduser.

Biological Information
Media Form	Liquid
Stem Cell Type	Mouse Embryonic Stem Cells Human Embryonic Stem Cells Mesenchymal Stem Cells Neural Stem Cells Hematopoietic Stem Cells Epithelial Cells Pancreatic Stem Cells Cardiac Stem Cells Induced Pluripotent Stem Cells

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable when stored at -20°C. Refer to lot expiration date. Recommended storage upon receipt is -20°C. After thawing, ACCUTASE may be stored for up to 2 months at 4°C. DO NOT STORE AT ROOM TEMP

Packaging Information
Material Size	100 mL

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
Catalog Number	GTIN
SCR005	04053252267819

Documentation

Accutase cell detachment solution SDS

Title
Safety Data Sheet (SDS)

Accutase cell detachment solution Certificates of Analysis

Title	Lot Number
ACCUTASE SOLUTION - 1970316	1970316
ACCUTASE SOLUTION - 2000796	2000796
ACCUTASE SOLUTION - 2022641	2022641
ACCUTASE SOLUTION - 2058324	2058324
ACCUTASE SOLUTION - 2092639	2092639
ACCUTASE SOLUTION - 2138544	2138544
ACCUTASE SOLUTION - 2197143	2197143
ACCUTASE SOLUTION	2943256
ACCUTASE SOLUTION	3062023
ACCUTASE SOLUTION	3007963

References

Reference overview	Pub Med ID
HDAC1 regulates pluripotency and lineage specific transcriptional networks in embryonic and trophoblast stem cells. Kidder B. L. & Palmer S. Nucleic Acids Res. 1-15 2011 Show Abstract Epigenetic regulation of gene expression is important in maintaining self-renewal of embryonic stem (ES) and trophoblast stem (TS) cells. Histone deacetylases (HDACs) negatively control histone acetylation by removing covalent acetylation marks from histone tails. Because histone acetylation is a known mark for active transcription, HDACs presumably associate with inactive genes. Here, we used genome-wide chromatin immunoprecipitation to investigate targets of HDAC1 in ES and TS cells. Through evaluation of genes associated with acetylated histone H3 marks, and global expression analysis of Hdac1 knockout ES and trichostatin A-treated ES and TS cells, we found that HDAC1 occupies mainly active genes, including important regulators of ES and TS cells self-renewal. We also observed occupancy of methyl-CpG binding domain protein 3 (MBD3), a subunit of the nucleosome remodeling and histone deacetylation (NuRD) complex, at a subset of HDAC1-occupied sequences in ES cells, including the pluripotency regulators Oct4, Nanog and Kfl4. By mapping HDAC1 targets on a global scale, our results describe further insight into epigenetic mechanisms of ES and TS cells self-renewal.	22156375

Reference overview

Pub Med ID

Brochure

Title
Murine Embryonic Stem Cell Culture

Data Sheet

Title
ACCUTASE™ SOLUTION

FAQ

Question	Answer
What is the difference between Accuma and Accutase?	Accumax (Catalog #SCR006) will dissociate clumped cells in minutes resulting in a single cell suspension. It will yield accurate reproducible cell counts, is cost effective and is ready to use. The Accutase (Catalog #SCR005) contains phenol red. It will detect adherent cells within minutes and will dissociate tissues for primary cell culture use. It also will yield accurate reproducible cell counts, is cost effective and is ready to use.

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SCR005 MilliporeAccutase cell detachment solution

A cell detachment solution of proteolytic & collagenolytic enzymes. The reagent is useful for creating single cell suspensions from clumped cell cultures for accurate cell counting, detachment of cells from primary tissue.

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Documentation

Accutase cell detachment solution SDS

Accutase cell detachment solution Certificates of Analysis

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A cell detachment solution of proteolytic & collagenolytic enzymes. The reagent is useful for creating single cell suspensions from clumped cell cultures for accurate cell counting, detachment of cells from primary tissue.

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Accutase cell detachment solution SDS

Accutase cell detachment solution Certificates of Analysis

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