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ABC1411-25UL Anti-AML1-ETO

Anti-AML1-ETO, Cat. No. ABC1411, is a rabbit polyclonal antibody that detects AML1-ETO and is tested for use in ChIP-seq, Immunoprecipitation, and Western Blotting.

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ABC1411-25UL
25 µL  
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      Overview

      Replacement Information
      Description
      Catalogue NumberABC1411-25UL
      DescriptionAnti-AML1-ETO
      Alternate Names
      • Protein CBFA2T1
      • Cyclin-D-related protein
      • Eight twenty one protein
      • Protein ETO
      • Protein MTG8
      • Zinc finger MYND domain-containing protein 2
      Background InformationProtein CBFA2T1 (UniProt: Q06455; also known as Cyclin-D-related protein, Eight twenty one protein, Protein ETO, Protein MTG8, Zinc finger MYND domain-containing protein 2) is encoded by the RUNX1T1 (also known as AML1T1, CBFA2T1, CDR, ETO, MTG8, ZMYND2) gene (Gene ID: 862) in human. In acute myeloid leukemias (AMLs) a recurring chromosomal translocation termed as t(8;21) is reported to generates the AML1 (8:21) ETO leukemia fusion protein that contains the DNA-binding domain of 1 transcription factor RUNX1 and almost all of ETO. It is shown that RUNX1 and the AML1 ETO fusion protein are co-expressed in t(8;21) AML cells and antagonize each other s gene-regulatory functions by co-occupying the binding sites of AML1 ETO activated genes. Both AML1-ETO and the AML1 protein are reported to recognize the same consensus DNA-binding motif (TGT/CGGT). AML1 ETO can either activate or repress transcription of different target genes. It can activate transcription of genes via recruitment of p300 and can repress transcription by competitively displacing RUNX1 and recruiting corepressors such as histone deacetylase 3 (HDAC3). Myeloid leukemia cells with the t(8;21) translocation, such as Kasumi-1 cells, display elevated levels of Bcl-2 that prolongs their life span and contribute to the development of t(8;21) leukemia. (Ref.: Guo, C., et al. (2020). J. Biol. Chem. 295(13) 4212 4223; Klampfer, L., et al. (1996). Proc Natl Acad Sci USA. 93(24); 14059-14064).
      References
      Product Information
      FormatSerum
      PresentationRabbit polyclonal antibody in serum with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-AML1-ETO, Cat. No. ABC1411, is a rabbit polyclonal antibody that detects AML1-ETO and is tested for use in ChIP-seq, Immunoprecipitation, and Western Blotting.
      Key Applications
      • Western Blotting
      • Chromatin Immunoprecipitation (ChIP)
      • ChIP-seq
      Application NotesTested Applications

      Immunoprecipitation Analysis: A representative lot immunoprecipitated AML1-ETO in Kasumi-1; t(8;21) cells expressing AML1-ETO (Courtesy of Dr Jinsong Zhang @ Saint Louis University).

      Chromatin immunoprecipitation (ChIP): A representative lot detected AML1-ETO in Kasumi-1; t(8;21) cells expressing AML1-ETO (Courtesy of Dr Jinsong Zhang @ Saint Louis University).

      ChIP-seq: A representative lot detected AML1-ETO in ChIP-seq applications (Guo, Chun., et. al. (2020). J Biol Chem. 295(13); 4212-4223.

      Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected AML1-ETO in Chromatin Immunoprecipitation applications (Guo, Chun., et. al. (2020). J Biol Chem. 295(13):4212-4223; Steinauer, Nickolas., et. al. (2019). Blood Adv. 3(9): 1379-1393).


      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to 17 amino acids from the C-terminal region of human ETO protein fused to AML1.
      EpitopeC-terminal
      ConcentrationPlease refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody detects ETO protein and AML-1-ETO fusion protein. It targets an epitope within 17 amino acids from the C-terminal region.
      IsotypeIgG
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Predicted to react with Mouse, Rat based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • RUNX1T1
      Purification MethodUnpurified
      UniProt Number
      Molecular Weight~62 and 100 kDa observed; 67.57 and 48.74 kDa for ETO and AML-1 protein, respectively calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in Kasumi-1; t(8;21) cells expressing AML1-ETO.

      Western Blotting Analysis: A 1:1,000 dilution of this antibody detected AML1-ETO in Kasumi-1 ; t(8;21) cells expressing AML1-ETO.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size25 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      ABC1411-25UL 04065266060133