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CBL171-I Anti-Actin, smooth muscle Antibody, clone ASM-1/1A4

Anti-Actin, smooth muscle Antibody, clone ASM-1/1A4 is an antibody against Actin for use in Western Blotting, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, ELISA.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Research Sub-Category: Cytoskeletal Signaling Gene Symbol: ACTA2, AAT6, ACTSA, ACTVS, GIG46, MYMY5 UniProt Number: P62736
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CBL171-I
100 μg  
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      Overview

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      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, R, Ch, BWB, IHC, ICC, IF, ELISAMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberCBL171-I
      DescriptionAnti-Actin, smooth muscle Antibody, clone ASM-1/1A4
      Alternate Names
      • Actin, aortic smooth muscle
      • Alpha-actin-2
      • Cell growth-inhibiting gene 46 protein
      Background InformationActin, aortic smooth muscle (UniProt P62736; also known as Alpha-actin-2, Cell growth-inhibiting gene 46 protein) is encoded by the ACTA2 (also known as AAT6, ACTSA, ACTVS, GIG46, MYMY5) gene (Gene ID 59) in human. Actins are globular multi-functional proteins that serve as the basic building blocks of cytoskeletal microfilaments and are among the most conserved eukaryotic proteins. Six actin types exist, skeletal muscle alpha-actin is encoded by the ACTA1 gene, smooth muscle alpha-actin by the ACTA2 gene, cytoplasmic beta-actin by the ACTB gene, cardiac muscle alpha-actin by the ACTC1 gene, cytoplasmic gamma-actin by the ACTG1 gene, and smooth muscle gamma-actin by the ACTG2 (a.k.a. ACTA3) gene. Although actins show >90% overall sequence homology, isoforms do show spatial, temporal, and tissue-specific expresson patterns and only 50-60% homology is found in their 18 N-terminal residues. Cytoplasmic β and γ-actins, are thought to be present in all cells, while the other four actin isoforms are typically found in specific adult muscle tissue types. Actins exist in a variety of structural states, depending on the specific ionic conditions or the interaction with ligand proteins. The oligomeric and polymeric forms that actin molecules assume are dependent on the distinct conformations they adopt.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Actin, smooth muscle Antibody, clone ASM-1/1A4 is an antibody against Actin for use in Western Blotting, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, ELISA.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      • Immunocytochemistry
      • Immunofluorescence
      • ELISA
      Application NotesWestern Blotting Analysis: A representative lot detected full-length and caspse-3-cleaved smooth muscle actin in uterus extracts from pregnant mice (Jeyasuria, P., et al. (2009). Biol. Reprod. 80(5):928-934).
      Western Blotting Analysis: A representative lot detected smooth muscle actin in PC-3M-LN4 and PC-3M-LN4-derived human prostate cancer cells (Zvieriev, V., et al. (2005). Biochem. Biophys. Commun. 337(2):498-504).
      Western Blotting Analysis: A representative lot detected developing stage-dependent vascular alpha-actin level in chicken heart of various somite stages (Ehler, E., et al. (2004). Dev. Dyn. 229(4):745-755).
      Western Blotting Analysis: A representative lot detected aortic actin, but not actin from fibroblasts (beta- and gamma-cytoplasmic), striated muscle (alpha-sarcomeric), or myocardium (alpha-myocardial) in various human, rat, bovine and rabbit tissue and cell lysates (Skalli, O., et al. (1986). J. Cell Biol. 103(6 Pt 2):2787-2796).
      Immunohistochemistry Analysis: A representative lot immunostained smooth muscle actin-positive myofibroblasts in paraformaldehyde-fix rat skin wounds sections (Mirastschijski, U., et al. (2010). Wound Repair Regen. 18(2):223-234).
      Immunohistochemistry Analysis: A representative lot immunostained activated myofibroblasts in formalin-fixed, paraffin-embedded mouse submandibular salivary gland (Hall, B.E., et al. (2010). Lab. Invest. 90(4):543-555).
      Immunohistochemistry Analysis: A representative lot immunostained α-SMA-positive cells in paraffin-embedded rat healing patellar tendon sections (Fu, S.C., et al. (2008). J. Orthop. Res. 26(3):374-383).
      Immunocytochemistry Analysis: Representative lots immunostained the mesoderm layer of embryoid bodies formed in vitro from northern white rhinoceros iPSCs, as well as murine iPSCs and mESCs (Ben-Nun, I.F., et al. (2011). Nat. Methods. 8(10):829-831; Gerlach, J.C., et al. (2010). Cells Tissues Organs. 192(1):39-49; Shao, L., et al. (2009). Cell Res. 19(3):296-306).
      Immunohistochemistry Analysis: A representative lot immunostained a subsets of cultured rat aortic medial smooth muscle cells (SMCs) and dermal fibroblasts (Skalli, O., et al. (1986). J. Cell Biol. 103(6 Pt 2):2787-2796).
      Immunohistochemistry Analysis: A representative lot immunostained cultured rat tendon cells (Fu, S.C., et al. (2008). J. Orthop. Res. 26(3):374-383).
      Immunofluorescence Analysis: A representative lot immunostained myofibrils using methanol-fixed, 9-somite stage chicken heart whole-mount preparations. The vascular alpha-actin staining started to disappear from a subset of the myofibrils when using 10-somite stage chicken heart preparations (Ehler, E., et al. (2004). Dev. Dyn. 229(4):745-755).
      Immunofluorescence Analysis: A representative lot immunostained stromal vessel SM cells in acetone-fixed benign (leiomyomas) and malignant (leiomyosarcomas & intravascular leiomyomatosis) smooth muscle (SM) neoplasms cryosections (Schürch, W., et al. (1987). Am. J. Pathol. 128(1):91-103).
      Immunofluorescence Analysis: A representative lot immunostained chicken gizzard and rat myocardium blood vessels, as well as smooth muscle cells (SMCs) in various acetone-fixed human and rat cryosections, whereas chicken gizzard parenchyrnal cells, rat cardiocytes, aorta endothelial cells and adventitial fibroblasts were negative (Skalli, O., et al. (1986). J. Cell Biol. 103(6 Pt 2):2787-2796).
      ELISA Analysis: A representative lot detected aortic actin, but not actin from fibroblasts (beta- and gamma-cytoplasmic), striated muscle (alpha-sarcomeric), or myocardium (alpha-myocardial) by ELISA (Skalli, O., et al. (1986). J. Cell Biol. 103(6 Pt 2):2787-2796).
      Biological Information
      ImmunogenBSA-conjugated linear peptide corresponding to the N-terminal sequence of human smooth muscle actin.
      EpitopeN-terminus.
      CloneASM-1 (1A4)
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone ASM-1, also known as anti-αsm-1 and clone 1A4, reacted with aortic actin, but not actin from fibroblasts (β- and γ-cytoplasmic), striated muscle (α-sarcomeric), or myocardium (α-myocardial) by ELISA and Western blotting (Skalli, O., et al. (1986). J. Cell Biol. 103(6 Pt 2):2787-2796).
      IsotypeIgG2aκ
      Species Reactivity
      • Human
      • Mouse
      • Rat
      • Chicken
      • Bovine
      Species Reactivity NoteHuman, Mouse, Rat, Chicken, Bovine. Predicted to react with Equine based on 100% sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • ACTA2
      • AAT6
      • ACTSA
      • ACTVS
      • GIG46
      • MYMY5
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~45 kDa observed.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HUVEC lysate.

      Western Blotting Analysis: 0.5 µg/mL of this antibody detected smooth muscle actin in 10 µg of HUVEC lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      CBL171-I 04055977330168

      Documentation

      Anti-Actin, smooth muscle Antibody, clone ASM-1/1A4 Certificates of Analysis

      TitleLot Number
      Anti-Actin, smooth muscle, 3073846
      Anti-Actin, smooth muscle, -Q2659765 Q2659765
      Anti-Actin, smooth muscle, clone ASM-1/1 Monoclonal Antibody 2879547
      Anti-Actin, smooth muscle, clone ASM-1/1A4 - 3308176 3308176
      Anti-Actin, smooth muscle, clone ASM-1/1A4 - 3393260 3393260
      Anti-Actin, smooth muscle, clone ASM-1/1A4 - 3700353 3700353
      Anti-Actin, smooth muscle, clone ASM-1/1A4 - 3779083 3779083
      Anti-Actin, smooth muscle, clone ASM-1/1A4 - 3847944 3847944
      Anti-Actin, smooth muscle, clone ASM-1/1A4 - 3977628 3977628
      Anti-Actin, smooth muscle, clone ASM-1/1A4 Monoclonal Antibody 2912582

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies