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MAB131884-25UL Anti-BASE-tag Antibody, clone 7C7

Anti-BASE-tag, clone 7C7, Cat. No. MAB131884, is a mouse monoclonal antibody that recognizes the epitope QPAKKRLNFGQTGD and has been tested for use in Western Blotting, Immunoprecipitation, and Immunofluorescence.

Anti-BASE-tag Antibody, clone 7C7 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Inflammation & Immunology Gene Symbol: Cap UniProt Number: Q6JC40
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MAB131884-25UL
25 µL  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      VirusIF, IP, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMAB131884-25UL
      DescriptionAnti-BASE-tag Antibody, clone 7C7
      Alternate Names
      • Capsid protein VP1
      • Capsid protein AVV9
      Background InformationCapsid protein VP1 (UniProt: Q6JC40) is encoded by the Cap gene in Adeno-associated virus 9 (AAV9). Adeno-associated viruses (AAV) are small non-enveloped DNA viruses that can serve as promising viral vectors for gene delivery, but can also elicits cellular and humoral immune responses. AAVs are reported to be nonpathogenic in humans and animals and display low immunogenicity. Following their attachment to cell surface receptor they are internalized via endocytosis and their intracellular trafficking is a rate-limiting, slow and inefficient process. AAVs contain an icosahedral capsid, which is 22 to 26 nm in diameter. They are shown to have a single-stranded DNA genome of approximately 4.7 kilobases that is flanked by two T-shaped inverted terminal repeats (ITRs). The 7C7 an epitope-tag antibody recognizes a basic ten amino acid epitope (QPAKKRLNFGQTGD) with high specificity. The basic nature of the BASE-tag epitope sequence distinguishes it from other commonly used epitope tags, such as Myc, EQKLISEEDL, FLAG, DYKDDDDK, HA, YPYDVPDYA, V5 etc. Clone 7C7 recognizes capsid proteins VP1 and VP2, but not VP3. Based on sequence identity of the epitope sequence, this antibody is expected to recognize VP1 and VP2 of AAV serotypes 1,2,3,6,7,8, and 9. (Ref.: Nonnenmacher, M., and Weber, T (2012). Gene Ther. 19(6); 649-658).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-BASE-tag, clone 7C7, Cat. No. MAB131884, is a mouse monoclonal antibody that recognizes the epitope QPAKKRLNFGQTGD and has been tested for use in Western Blotting, Immunoprecipitation, and Immunofluorescence.
      Key Applications
      • Immunofluorescence
      • Immunoprecipitation
      • Western Blotting
      Application NotesImmunoprecipitation: A representative lot immunoprecipitated EGFP-BASE-tag-Actin in HEK293T cells transfected with EGFP-BASE-tag-Actin (Courtesy: Dr. Thomas Weber, Icahn School of Medicine at Mount Sinai, New York).
      Immunofluorescence: A representative lot detected EGFP-BASE-tag-Actin in HeLa cells transfected with EGFP-BASE-tag-Actin (Courtesy: Dr. Thomas Weber, Icahn School of Medicine at Mount Sinai, New York).
      Biological Information
      ImmunogenEmpty Adeno-associated virus 9 (AAV9) Capsids
      Clone7C7
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 7C7 is a mouse monoclonal antibody that detects various serotypes of Adeno-associated virus (AVV). It targets an epitope with in 14 amino acids from the N-terminal region.
      IsotypeIgG2bκ
      Species Reactivity
      • Virus
      Species Reactivity NoteVirus (Adenovirus)
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • Cap
      Purification MethodProtein G purified
      UniProt Number
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HEK293 cells transfected with GFP-7C7-Actin.

      Western Blotting Analysis: A 1:500 dilution of this antibody detected BASE-tag in HEK293 cells transfected with GFP-7C7-Actin.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size25 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      MAB131884-25UL 04054839563386