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MABE60 Anti-BRG1 Antibody, clone 3G4

Anti-BRG1 Antibody, clone 3G4 is a rat monoclonal Antibody for detection of BRG1, also known as BRG1-associated factor 190A, SNF2-like 4, and SNF2-BETA, and has been validated in Immunocytochemistry and Western Blotting applications.

Anti-BRG1 Antibody, clone 3G4 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Chromatin Biology, Transcription Factors Gene Symbol: SMARCA4, BAF190A, BRG1, MRD16, RTPS2, SNF2B, SNF2L4 UniProt Number: P51532
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MABE60
100 µg  
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      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MICC, WBRPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABE60
      DescriptionAnti-BRG1 Antibody, clone 3G4
      Alternate Names
      • Transcription activator BRG1
      • ATP-dependent helicase SMARCA4
      • BAF190A
      • BRG1-associated factor 190A
      • hSNF2b
      • Mitotic growth and transcription activator
      • Protein brahma homolog 1
      • Protein BRG-1
      • SNF2-beta
      • SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 4
      Background InformationTranscription activator BRG1 (UniProt P51532; also known as ATP-dependent helicase SMARCA4, BAF190A, BRG1-associated factor 190A, hSNF2b, Mitotic growth and transcription activator, Protein brahma homolog 1, Protein BRG-1, SNF2-beta, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 4) is encoded by the SMARCA4 (also known as BAF190A, BRG1, MRD16, RTPS2, SNF2B, SNF2L4) gene (Gene ID 6597) in human. The SWI/SNF-like BRG1/BRM-associated factor (BAF) chromatin-remodeling complexes drive conformational changes of nucleosomes in an ATP-dependent manner and play important roles in cell-cycle progression, DNA repair, and development. The SWI/SNF complexes contain 10-12 interchangeable subunits, with either BRG1/SMARCA4 or BRM/SMARCA2 as the ATPase subunit. Brg1 is also shown to maintain Polycomb-mediated repression of non-mesodermal developmental regulators. Brg1-knockout leads to disruption of murine ESC cardiomyocyte differentiation and dysregulation of lineage-specific gene expression during mesoderm induction.
      References
      Product Information
      FormatPurified
      Control
      • HeLa cell lysate
      PresentationPurified rat monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-BRG1 Antibody, clone 3G4 is a rat monoclonal Antibody for detection of BRG1, also known as BRG1-associated factor 190A, SNF2-like 4, and SNF2-BETA, and has been validated in Immunocytochemistry and Western Blotting applications.
      Key Applications
      • Immunocytochemistry
      • Western Blotting
      Application NotesWestern Blotting Analysis: A representative lot detected BRG1 (SMARCA4) in THP-1 human leukemia monocytic cell lysates (Cruickshank, V.A., et al. (2015). PLoS One. 10(11):e0142806).

      Immunocytochemistry Analysis: A representative lot detected nuclear euchromatin BRG1 immunoreactivity in C2C12 murine myoblasts (Ohkawa, Y., et al. (2009). Hybridoma (Larchmt). 28(6):463-466).
      Biological Information
      ImmunogenGST-tagged recombinant protein corresponding to human BRG1.
      Clone3G4
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRat
      IsotypeIgG1κ
      Species Reactivity
      • Human
      • Mouse
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • SMARCA4
      • BAF190A
      • BRG1
      • MRD16
      • RTPS2
      • SNF2B
      • SNF2L4
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~185 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in HeLa cell lysate.

      Western Blot Analysis: 0.25 µg/mL of this antibody detected BRG1 in 10 µg of HeLa cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      MABE60 04053252747021

      Documentation

      Anti-BRG1 Antibody, clone 3G4 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-BRG1 Antibody, clone 3G4 Certificates of Analysis

      TitleLot Number
      Anti-BRG1, clone 3G4 - 2207807 2207807
      Anti-BRG1, clone 3G4 - 2291727 2291727
      Anti-BRG1, clone 3G4 - 3925336 3925336
      Anti-BRG1, clone 3G4 - 4052786 4052786
      Anti-BRG1, clone 3G4 - 4094577 4094577
      Anti-BRG1, clone 3G4 - NRG1782322 NRG1782322
      Anti-BRG1, clone 3G4 -2688653 2688653
      Anti-BRG1, clone 3G4 -2724251 2724251
      Anti-BRG1, clone 3G4 Monoclonal Antibody 3098928
      Anti-BRG1, clone 3G4 Monoclonal Antibody 3098927
      Show more

      References

      Reference overviewApplicationPub Med ID
      Production of a rat monoclonal antibody against Brg1.
      Ohkawa, Yasuyuki, et al.
      Hybridoma (Larchmt), 28: 463-6 (2009)  2009

      Show Abstract       		Immunocytochemistry20025509 20025509

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies